Proline production by transgenic plants of Swingle citrumelo and susceptibility to glufosinate ammonium

Bibliographic Details
Main Author: Carneiro, Cristine Elizabeth Alvarenga
Publication Date: 2006
Other Authors: Molinari, Hugo Bruno Correa, Andrade, Giselly Aparecida, Pereira, Luiz Filipe Protasio, Vieira, Luiz Gonzaga Esteves
Format: Article
Language: por
Source: Pesquisa Agropecuária Brasileira (Online)
Download full: https://seer.sct.embrapa.br/index.php/pab/article/view/7202
Summary: The objective of this work was to evaluate the susceptibility to glufosinate ammonium of transgenic plants of Swingle citrumelo with high proline production. The mutant gene of the enzyme D1-pyrroline-5-carboxylate synthetase (P5CS), the rate-limiting enzyme in proline biosynthesis, was inserted into Swingle citrumelo plants. The expression of the gene p5cs caused up to 5-fold increase on the proline content in leaf tissues of transgenic plants treated with 200 mM glufosinate ammonium, when compared with control plants. Leaves of transgenic plants accumulated higher amounts of NH4+ than the nontransgenic control. The herbicide toxicity was evaluated using leaf disks cultivated in MS medium, containing different concentrations of glufosinate ammonium. The severity of the chlorosis, observed in leaf disks of transgenic plants, confirmed the higher susceptibility of Swingle citrumelo plants, with high proline production, to this herbicide.
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spelling Proline production by transgenic plants of Swingle citrumelo and susceptibility to glufosinate ammoniumProdução de prolina e suscetibilidade ao glufosinato de amônio em plantas transgênicas de citrumelo SwingleCitrus paradisi; Poncirus trifoliata; phosphinothricin; P5CSCitrus paradisi; Poncirus trifoliata; fosfinotricina; P5CSThe objective of this work was to evaluate the susceptibility to glufosinate ammonium of transgenic plants of Swingle citrumelo with high proline production. The mutant gene of the enzyme D1-pyrroline-5-carboxylate synthetase (P5CS), the rate-limiting enzyme in proline biosynthesis, was inserted into Swingle citrumelo plants. The expression of the gene p5cs caused up to 5-fold increase on the proline content in leaf tissues of transgenic plants treated with 200 mM glufosinate ammonium, when compared with control plants. Leaves of transgenic plants accumulated higher amounts of NH4+ than the nontransgenic control. The herbicide toxicity was evaluated using leaf disks cultivated in MS medium, containing different concentrations of glufosinate ammonium. The severity of the chlorosis, observed in leaf disks of transgenic plants, confirmed the higher susceptibility of Swingle citrumelo plants, with high proline production, to this herbicide.O objetivo deste trabalho foi avaliar a sensibilidade de plantas transgênicas de citrumelo Swingle com elevada produção de prolina, ao herbicida glufosinato de amônio. As plantas utilizadas apresentavam a inserção do gene mutante da enzima D1-pirrolina-5-carboxilato sintetase (P5CS), responsável pela biossíntese de prolina. A expressão do gene p5cs em plantas transgênicas causou aumento nas quantidades de prolina em tecidos foliares, em até cinco vezes, quando comparadas às plantas-controle tratadas com 200 mM de glufosinato de amônio. As plantas transgênicas acumularam maior quantidade de NH4+ nas folhas, em relação às plantas não-transgênicas. Os danos causados pelo herbicida foram avaliados in vitro, utilizando-se discos foliares cultivados em meio MS com diferentes concentrações de glufosinato de amônio. Observou-se maior clorose em discos foliares das plantas transgênicas, o que comprova a maior suscetibilidade de plantas de citrumelo Swingle com alta produção de prolina ao herbicida.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCarneiro, Cristine Elizabeth AlvarengaMolinari, Hugo Bruno CorreaAndrade, Giselly AparecidaPereira, Luiz Filipe ProtasioVieira, Luiz Gonzaga Esteves2006-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/7202Pesquisa Agropecuaria Brasileira; v.41, n.5, maio 2006; 747-753Pesquisa Agropecuária Brasileira; v.41, n.5, maio 2006; 747-7531678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/7202/4247info:eu-repo/semantics/openAccess2014-09-29T18:21:07Zoai:ojs.seer.sct.embrapa.br:article/7202Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-09-29T18:21:07Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Proline production by transgenic plants of Swingle citrumelo and susceptibility to glufosinate ammonium
Produção de prolina e suscetibilidade ao glufosinato de amônio em plantas transgênicas de citrumelo Swingle
title Proline production by transgenic plants of Swingle citrumelo and susceptibility to glufosinate ammonium
spellingShingle Proline production by transgenic plants of Swingle citrumelo and susceptibility to glufosinate ammonium
Carneiro, Cristine Elizabeth Alvarenga
Citrus paradisi; Poncirus trifoliata; phosphinothricin; P5CS
Citrus paradisi; Poncirus trifoliata; fosfinotricina; P5CS
title_short Proline production by transgenic plants of Swingle citrumelo and susceptibility to glufosinate ammonium
title_full Proline production by transgenic plants of Swingle citrumelo and susceptibility to glufosinate ammonium
title_fullStr Proline production by transgenic plants of Swingle citrumelo and susceptibility to glufosinate ammonium
title_full_unstemmed Proline production by transgenic plants of Swingle citrumelo and susceptibility to glufosinate ammonium
title_sort Proline production by transgenic plants of Swingle citrumelo and susceptibility to glufosinate ammonium
author Carneiro, Cristine Elizabeth Alvarenga
author_facet Carneiro, Cristine Elizabeth Alvarenga
Molinari, Hugo Bruno Correa
Andrade, Giselly Aparecida
Pereira, Luiz Filipe Protasio
Vieira, Luiz Gonzaga Esteves
author_role author
author2 Molinari, Hugo Bruno Correa
Andrade, Giselly Aparecida
Pereira, Luiz Filipe Protasio
Vieira, Luiz Gonzaga Esteves
author2_role author
author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Carneiro, Cristine Elizabeth Alvarenga
Molinari, Hugo Bruno Correa
Andrade, Giselly Aparecida
Pereira, Luiz Filipe Protasio
Vieira, Luiz Gonzaga Esteves
dc.subject.por.fl_str_mv Citrus paradisi; Poncirus trifoliata; phosphinothricin; P5CS
Citrus paradisi; Poncirus trifoliata; fosfinotricina; P5CS
topic Citrus paradisi; Poncirus trifoliata; phosphinothricin; P5CS
Citrus paradisi; Poncirus trifoliata; fosfinotricina; P5CS
description The objective of this work was to evaluate the susceptibility to glufosinate ammonium of transgenic plants of Swingle citrumelo with high proline production. The mutant gene of the enzyme D1-pyrroline-5-carboxylate synthetase (P5CS), the rate-limiting enzyme in proline biosynthesis, was inserted into Swingle citrumelo plants. The expression of the gene p5cs caused up to 5-fold increase on the proline content in leaf tissues of transgenic plants treated with 200 mM glufosinate ammonium, when compared with control plants. Leaves of transgenic plants accumulated higher amounts of NH4+ than the nontransgenic control. The herbicide toxicity was evaluated using leaf disks cultivated in MS medium, containing different concentrations of glufosinate ammonium. The severity of the chlorosis, observed in leaf disks of transgenic plants, confirmed the higher susceptibility of Swingle citrumelo plants, with high proline production, to this herbicide.
publishDate 2006
dc.date.none.fl_str_mv 2006-05-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7202
url https://seer.sct.embrapa.br/index.php/pab/article/view/7202
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7202/4247
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.41, n.5, maio 2006; 747-753
Pesquisa Agropecuária Brasileira; v.41, n.5, maio 2006; 747-753
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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