Identification and purification of a granulosis virus found in fall armyworms

Detalhes bibliográficos
Autor(a) principal: Valicente, Fernando H.
Data de Publicação: 2014
Outros Autores: Peixoto, Maria José V. de V. Diniz, Paiva, Edilson, Kitajima, Elliot W.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/13842
Resumo: A virus was found infecting larvae of fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) (Smith, 1797), in Sete Lagoas, MG, Brazil. Virus extracts from infected larvae were able to cause up to 100% mortality in three-day old larvae feeding in artificial diet. The identification of the virus, made by electron microscopy, showed that it was a granulosis virus, belonging to the genus Baculovirus and is composed of enveloped nucleocapsids which are occluded in proteinaceous inclusion bodies. Purifications by differential and sucrose gradient centrifugations yielded 22 mg of inclusion bodies per infected larva. Due to its pathogenicity, large number of inclusion bodies per infected larvae and simplicity of purification procedures, the identified virus shows a great potential to be used as a bioinsecticide in the biological control of the fall armyworm. 
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spelling Identification and purification of a granulosis virus found in fall armywormsIdentificação e purificação de um vírus-de-granulose em lagartas-do-cartucho-do-milhoZea mays; insect pathology; corn; Baculovirus; inclusion bodiesZea mays; patologia de insetos; milho; Baculovirus; corpos de inclusãoA virus was found infecting larvae of fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) (Smith, 1797), in Sete Lagoas, MG, Brazil. Virus extracts from infected larvae were able to cause up to 100% mortality in three-day old larvae feeding in artificial diet. The identification of the virus, made by electron microscopy, showed that it was a granulosis virus, belonging to the genus Baculovirus and is composed of enveloped nucleocapsids which are occluded in proteinaceous inclusion bodies. Purifications by differential and sucrose gradient centrifugations yielded 22 mg of inclusion bodies per infected larva. Due to its pathogenicity, large number of inclusion bodies per infected larvae and simplicity of purification procedures, the identified virus shows a great potential to be used as a bioinsecticide in the biological control of the fall armyworm. Foi constatada a presença de um vírus-de-granulose (VG) infectando lagartas-do-cartucho-do-milho, Spodoptera frugiperda (Lepidoptera: Noctuidae) (Smith, 1797), VGSf, na região de Sete Lagoas, MG. Extratos de lagartas infectadas com vírus mostraram ser patogênicos, chegando a causar até 100% de mortalidade em lagartas de três dias de idade, criadas artificialmente em laboratório. A identificação do vírus foi feita através de microscopia eletrônica, e os resultados mostraram tratar-se de um vírus-de-granulose, o qual pertence ao género Baculovirus e caracteriza-se por apresentar suas partículas oclusas individualmente em uma cápsula de proteína, formando estruturas características chamadas "corpos de inclusão" (CIs). A purificação do vírus, feita através de centrifugações diferenciais e em gradientes de sacarose, mostrou ser possível obter cerca de 22 mg de CIs do vírus por lagarta infectada. Dada a sua patogenicidade, grande quantidade de CIs por lagarta infectada e facilidade de purificação, o vírus em estudo apresenta um grande potencial para ser utilizado como bioinseticida no controle da lagarta-do-cartucho. Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraValicente, Fernando H.Peixoto, Maria José V. de V. DinizPaiva, EdilsonKitajima, Elliot W.2014-04-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/13842Pesquisa Agropecuaria Brasileira; v.23, n.3, mar. 1988; 291-296Pesquisa Agropecuária Brasileira; v.23, n.3, mar. 1988; 291-2961678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/13842/7852info:eu-repo/semantics/openAccess2014-09-08T18:04:56Zoai:ojs.seer.sct.embrapa.br:article/13842Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-09-08T18:04:56Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Identification and purification of a granulosis virus found in fall armyworms
Identificação e purificação de um vírus-de-granulose em lagartas-do-cartucho-do-milho
title Identification and purification of a granulosis virus found in fall armyworms
spellingShingle Identification and purification of a granulosis virus found in fall armyworms
Valicente, Fernando H.
Zea mays; insect pathology; corn; Baculovirus; inclusion bodies
Zea mays; patologia de insetos; milho; Baculovirus; corpos de inclusão
title_short Identification and purification of a granulosis virus found in fall armyworms
title_full Identification and purification of a granulosis virus found in fall armyworms
title_fullStr Identification and purification of a granulosis virus found in fall armyworms
title_full_unstemmed Identification and purification of a granulosis virus found in fall armyworms
title_sort Identification and purification of a granulosis virus found in fall armyworms
author Valicente, Fernando H.
author_facet Valicente, Fernando H.
Peixoto, Maria José V. de V. Diniz
Paiva, Edilson
Kitajima, Elliot W.
author_role author
author2 Peixoto, Maria José V. de V. Diniz
Paiva, Edilson
Kitajima, Elliot W.
author2_role author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Valicente, Fernando H.
Peixoto, Maria José V. de V. Diniz
Paiva, Edilson
Kitajima, Elliot W.
dc.subject.por.fl_str_mv Zea mays; insect pathology; corn; Baculovirus; inclusion bodies
Zea mays; patologia de insetos; milho; Baculovirus; corpos de inclusão
topic Zea mays; insect pathology; corn; Baculovirus; inclusion bodies
Zea mays; patologia de insetos; milho; Baculovirus; corpos de inclusão
description A virus was found infecting larvae of fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) (Smith, 1797), in Sete Lagoas, MG, Brazil. Virus extracts from infected larvae were able to cause up to 100% mortality in three-day old larvae feeding in artificial diet. The identification of the virus, made by electron microscopy, showed that it was a granulosis virus, belonging to the genus Baculovirus and is composed of enveloped nucleocapsids which are occluded in proteinaceous inclusion bodies. Purifications by differential and sucrose gradient centrifugations yielded 22 mg of inclusion bodies per infected larva. Due to its pathogenicity, large number of inclusion bodies per infected larvae and simplicity of purification procedures, the identified virus shows a great potential to be used as a bioinsecticide in the biological control of the fall armyworm. 
publishDate 2014
dc.date.none.fl_str_mv 2014-04-17
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/13842
url https://seer.sct.embrapa.br/index.php/pab/article/view/13842
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/13842/7852
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.23, n.3, mar. 1988; 291-296
Pesquisa Agropecuária Brasileira; v.23, n.3, mar. 1988; 291-296
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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