Identificação de acessos de mamoeiro resistentes ao Papaya lethal yellowing virus e capacidade de Tetranychus urticae em transmitir o vírus

Detalhes bibliográficos
Autor(a) principal: Basso, Marcos Fernando
Data de Publicação: 2015
Outros Autores: Pereira, Álvaro Júlio, Pereira, Hermano Monteiro de Barros, Ramos, Humberto Josué de Oliveira, Dantas, Jorge Luiz Loyola, Fontes, Elizabeth Pacheco Batista, Andrade, Eduardo Chumbinho de, Zerbini, Francisco Murilo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/19830
Resumo: The objective of this work was to produce a polyclonal antiserum against the coat protein (CP) of Papaya lethal yellowing virus (PLYV) and to determine its specificity and sensibility in the diagnosis of the virus, as well as to evaluate the genetic resistance to PLYV in papaya (Carica papaya) accessions and to investigate the capacity of the two‑spotted spider mite Tetranychus urticae to acquire and transmit PLYV to the plants. Sixty‑five papaya accessions were evaluated. For each accession, ten plants were mechanically inoculated using PLYV‑infected plant extracts, and three plants were mock inoculated with phosphate buffer alone and used as negative controls. Ninety days after inoculation, newly‑emerging systemic leaves were collected from the inoculated plants, and viral infection was diagnosed by indirect Elisa, using polyclonal antiserum sensible to the in vitro‑expressed PLYV CP. Viral transmission by T. urticae was evaluated in greenhouse. The experiments were repeated twice. Polyclonal antiserum recognized the recombinant PLYV CP specifically and discriminated PLYV infection from infections caused by other plant viruses. Out of the 65 papaya accessions evaluated, 15 were considered resistant, 18 moderately resistant, and 32 susceptible. The two‑spotted spider mite T. urticae was capable of acquiring PLYV, but not of transmitting it to papaya.
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spelling Identificação de acessos de mamoeiro resistentes ao Papaya lethal yellowing virus e capacidade de Tetranychus urticae em transmitir o vírusScreening of papaya accessions resistant to Papaya lethal yellowing virus and capacity of Tetranychus urticae to transmit the virusCarica papaya; Elisa; genetic resistance; plant breeding; PLYV; two-spotted spider miteCarica papaya; Elisa; resistência genética; melhoramento de plantas; PLYV; ácaro rajadoThe objective of this work was to produce a polyclonal antiserum against the coat protein (CP) of Papaya lethal yellowing virus (PLYV) and to determine its specificity and sensibility in the diagnosis of the virus, as well as to evaluate the genetic resistance to PLYV in papaya (Carica papaya) accessions and to investigate the capacity of the two‑spotted spider mite Tetranychus urticae to acquire and transmit PLYV to the plants. Sixty‑five papaya accessions were evaluated. For each accession, ten plants were mechanically inoculated using PLYV‑infected plant extracts, and three plants were mock inoculated with phosphate buffer alone and used as negative controls. Ninety days after inoculation, newly‑emerging systemic leaves were collected from the inoculated plants, and viral infection was diagnosed by indirect Elisa, using polyclonal antiserum sensible to the in vitro‑expressed PLYV CP. Viral transmission by T. urticae was evaluated in greenhouse. The experiments were repeated twice. Polyclonal antiserum recognized the recombinant PLYV CP specifically and discriminated PLYV infection from infections caused by other plant viruses. Out of the 65 papaya accessions evaluated, 15 were considered resistant, 18 moderately resistant, and 32 susceptible. The two‑spotted spider mite T. urticae was capable of acquiring PLYV, but not of transmitting it to papaya.O objetivo deste trabalho foi produzir um antissoro policlonal contra a proteína capsidial (PC) do Papaya lethal yellowing virus (PLYV) e determinar sua especificidade e sensibilidade na diagnose do vírus, bem como avaliar a resistência genética de acessos de mamoeiro (Carica papaya) ao PLYV e investigar a capacidade do ácaro rajado Tetranychus urticae em adquirir e transmitir o vírus às plantas. Foram avaliados 65 acessos de mamoeiro. Para cada acesso, dez plantas foram submetidas à inoculação mecânica com extratos de plantas infectadas com PLYV, e três plantas receberam inoculação apenas com tampão de fosfato e foram usadas como controle negativo. Noventa dias após a inoculação, novas folhas sistêmicas emergentes foram coletadas das plantas inoculadas, e a infecção viral foi diagnosticada por Elisa indireto, com uso de antissoro policlonal sensível à PC do PLYV expressa in vitro. A transmissão viral por T. urticae foi avaliada em casa de vegetação. Os experimentos foram repetidos duas vezes. O antissoro policlonal reconheceu a PC do PLYV especificamente e discriminou a infecção pelo PLYV de infecções causadas por outros vírus. Dos 65 acessos de mamoeiros avaliados, 15 foram considerados resistentes, 18 moderadamente resistentes e 32 suscetíveis. O ácaro rajado T. urticae foi capaz de adquirir o PLYV, mas não de transmiti‑lo para o mamoeiro.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraFAPEMIG, CAPES, CNPqBasso, Marcos FernandoPereira, Álvaro JúlioPereira, Hermano Monteiro de BarrosRamos, Humberto Josué de OliveiraDantas, Jorge Luiz LoyolaFontes, Elizabeth Pacheco BatistaAndrade, Eduardo Chumbinho deZerbini, Francisco Murilo2015-02-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/19830Pesquisa Agropecuaria Brasileira; v.50, n.2, fev. 2015; 97-105Pesquisa Agropecuária Brasileira; v.50, n.2, fev. 2015; 97-1051678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/19830/12862https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/19830/12047https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/19830/12048https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/19830/12049https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/19830/12050https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/19830/12056info:eu-repo/semantics/openAccess2015-02-27T12:14:49Zoai:ojs.seer.sct.embrapa.br:article/19830Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2015-02-27T12:14:49Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Identificação de acessos de mamoeiro resistentes ao Papaya lethal yellowing virus e capacidade de Tetranychus urticae em transmitir o vírus
Screening of papaya accessions resistant to Papaya lethal yellowing virus and capacity of Tetranychus urticae to transmit the virus
title Identificação de acessos de mamoeiro resistentes ao Papaya lethal yellowing virus e capacidade de Tetranychus urticae em transmitir o vírus
spellingShingle Identificação de acessos de mamoeiro resistentes ao Papaya lethal yellowing virus e capacidade de Tetranychus urticae em transmitir o vírus
Basso, Marcos Fernando
Carica papaya; Elisa; genetic resistance; plant breeding; PLYV; two-spotted spider mite
Carica papaya; Elisa; resistência genética; melhoramento de plantas; PLYV; ácaro rajado
title_short Identificação de acessos de mamoeiro resistentes ao Papaya lethal yellowing virus e capacidade de Tetranychus urticae em transmitir o vírus
title_full Identificação de acessos de mamoeiro resistentes ao Papaya lethal yellowing virus e capacidade de Tetranychus urticae em transmitir o vírus
title_fullStr Identificação de acessos de mamoeiro resistentes ao Papaya lethal yellowing virus e capacidade de Tetranychus urticae em transmitir o vírus
title_full_unstemmed Identificação de acessos de mamoeiro resistentes ao Papaya lethal yellowing virus e capacidade de Tetranychus urticae em transmitir o vírus
title_sort Identificação de acessos de mamoeiro resistentes ao Papaya lethal yellowing virus e capacidade de Tetranychus urticae em transmitir o vírus
author Basso, Marcos Fernando
author_facet Basso, Marcos Fernando
Pereira, Álvaro Júlio
Pereira, Hermano Monteiro de Barros
Ramos, Humberto Josué de Oliveira
Dantas, Jorge Luiz Loyola
Fontes, Elizabeth Pacheco Batista
Andrade, Eduardo Chumbinho de
Zerbini, Francisco Murilo
author_role author
author2 Pereira, Álvaro Júlio
Pereira, Hermano Monteiro de Barros
Ramos, Humberto Josué de Oliveira
Dantas, Jorge Luiz Loyola
Fontes, Elizabeth Pacheco Batista
Andrade, Eduardo Chumbinho de
Zerbini, Francisco Murilo
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv FAPEMIG, CAPES, CNPq

dc.contributor.author.fl_str_mv Basso, Marcos Fernando
Pereira, Álvaro Júlio
Pereira, Hermano Monteiro de Barros
Ramos, Humberto Josué de Oliveira
Dantas, Jorge Luiz Loyola
Fontes, Elizabeth Pacheco Batista
Andrade, Eduardo Chumbinho de
Zerbini, Francisco Murilo
dc.subject.por.fl_str_mv Carica papaya; Elisa; genetic resistance; plant breeding; PLYV; two-spotted spider mite
Carica papaya; Elisa; resistência genética; melhoramento de plantas; PLYV; ácaro rajado
topic Carica papaya; Elisa; genetic resistance; plant breeding; PLYV; two-spotted spider mite
Carica papaya; Elisa; resistência genética; melhoramento de plantas; PLYV; ácaro rajado
description The objective of this work was to produce a polyclonal antiserum against the coat protein (CP) of Papaya lethal yellowing virus (PLYV) and to determine its specificity and sensibility in the diagnosis of the virus, as well as to evaluate the genetic resistance to PLYV in papaya (Carica papaya) accessions and to investigate the capacity of the two‑spotted spider mite Tetranychus urticae to acquire and transmit PLYV to the plants. Sixty‑five papaya accessions were evaluated. For each accession, ten plants were mechanically inoculated using PLYV‑infected plant extracts, and three plants were mock inoculated with phosphate buffer alone and used as negative controls. Ninety days after inoculation, newly‑emerging systemic leaves were collected from the inoculated plants, and viral infection was diagnosed by indirect Elisa, using polyclonal antiserum sensible to the in vitro‑expressed PLYV CP. Viral transmission by T. urticae was evaluated in greenhouse. The experiments were repeated twice. Polyclonal antiserum recognized the recombinant PLYV CP specifically and discriminated PLYV infection from infections caused by other plant viruses. Out of the 65 papaya accessions evaluated, 15 were considered resistant, 18 moderately resistant, and 32 susceptible. The two‑spotted spider mite T. urticae was capable of acquiring PLYV, but not of transmitting it to papaya.
publishDate 2015
dc.date.none.fl_str_mv 2015-02-24
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/19830
url https://seer.sct.embrapa.br/index.php/pab/article/view/19830
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/19830/12862
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/19830/12047
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/19830/12048
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/19830/12049
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/19830/12050
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/19830/12056
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.50, n.2, fev. 2015; 97-105
Pesquisa Agropecuária Brasileira; v.50, n.2, fev. 2015; 97-105
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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