Genetic variability of Phakopsora pachyrhizi accessed by microsatellite markers

Detalhes bibliográficos
Autor(a) principal: Tschurtschenthaler, Nely Norder
Data de Publicação: 2012
Outros Autores: Vieira, Elisa Serra Negra, Dalla Nora, Tatiane, Schuster, Ivan
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/10933
Resumo: The objective of this work was to evaluate the genetic variability of Asian soybean rust in Brazil using microsatellite markers. Samples of Phakopsora pachyrhizi spores collected in the Southern, Southeastern, and Central West regions of Brazil were subjected to analysis of genetic variability, accessed with microsatellite markers specific for the fungus. Spores of the fungus were also collected in several soybean varieties in a same locality, including samples with reddish‑brown (RB) lesions. Among these samples, there was no variability. Tissues with RB lesions did not contain spores of the fungus and did not amplify with the specific markers for P. pachyrhizi. Genetic variability among samples collected in the three regions ranged from 0 to 0.36. A tendency to cluster samples of the Southern and Central West regions of Brazil in different groups was observed. The existence of genetic variability in P. pachyrhizi spore populations indicates that vertical genetic resistance, provided by single genes, is a risky strategy for soybean breeding programs that aim resistance to Asian soybean rust.
id EMBRAPA-4_912e43f5c58dadb039748a35d4664b7a
oai_identifier_str oai:ojs.seer.sct.embrapa.br:article/10933
network_acronym_str EMBRAPA-4
network_name_str Pesquisa Agropecuária Brasileira (Online)
repository_id_str
spelling Genetic variability of Phakopsora pachyrhizi accessed by microsatellite markersVariabilidade genética de Phakopsora pachyrhizi avaliada por meio de marcadores microssatélitesGlycine max; genetic diversity; Asian soybean rust; genetic resistance; vertical resistance; SSRGlycine max; diversidade genética; ferrugem‑asiática‑da‑soja; resistência genética; resistência vertical; SSRThe objective of this work was to evaluate the genetic variability of Asian soybean rust in Brazil using microsatellite markers. Samples of Phakopsora pachyrhizi spores collected in the Southern, Southeastern, and Central West regions of Brazil were subjected to analysis of genetic variability, accessed with microsatellite markers specific for the fungus. Spores of the fungus were also collected in several soybean varieties in a same locality, including samples with reddish‑brown (RB) lesions. Among these samples, there was no variability. Tissues with RB lesions did not contain spores of the fungus and did not amplify with the specific markers for P. pachyrhizi. Genetic variability among samples collected in the three regions ranged from 0 to 0.36. A tendency to cluster samples of the Southern and Central West regions of Brazil in different groups was observed. The existence of genetic variability in P. pachyrhizi spore populations indicates that vertical genetic resistance, provided by single genes, is a risky strategy for soybean breeding programs that aim resistance to Asian soybean rust.O objetivo deste trabalho foi avaliar a variabilidade genética da ferrugem‑asiática‑da‑soja no Brasil, com uso de marcadores microssatélites. Populações de esporos de Phakopsora pachyrhizi coletadas nas regiões Sul, Sudeste e Centro Oeste do país foram submetidas à análise de variabilidade genética, avaliada por meio de marcadores microssatélites específicos para o fungo. Foram coletadas, também, populações de fungo em diversas variedades de soja em uma mesma localidade, incluindo populações com lesão “reddish‑brown” (RB). Entre essas populações, não houve variabilidade. Tecidos com lesões RB não apresentaram esporos do fungo e não amplificaram com os marcadores específicos para P. pachyrhizi. A variabilidade genética entre as populações coletadas nas três regiões variou de 0 a 0,36. Observou-se tendência de agrupamento das populações da região Sul e Centro Oeste do Brasil em grupos diferentes. A existência de variabilidade genética em populações de P. pachyrhizi é um indicativo de que a resistência genética vertical, conferida por genes únicos, é uma estratégia de risco para os programas de melhoramento genético que visam a resistência à ferrugem‑asiática‑da‑soja no Brasil. Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraTschurtschenthaler, Nely NorderVieira, Elisa Serra NegraDalla Nora, TatianeSchuster, Ivan2012-04-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/10933Pesquisa Agropecuaria Brasileira; v.47, n.2, fev. 2012; 181-186Pesquisa Agropecuária Brasileira; v.47, n.2, fev. 2012; 181-1861678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/10933/6780https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/10933/6349info:eu-repo/semantics/openAccess2012-06-17T12:19:21Zoai:ojs.seer.sct.embrapa.br:article/10933Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2012-06-17T12:19:21Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Genetic variability of Phakopsora pachyrhizi accessed by microsatellite markers
Variabilidade genética de Phakopsora pachyrhizi avaliada por meio de marcadores microssatélites
title Genetic variability of Phakopsora pachyrhizi accessed by microsatellite markers
spellingShingle Genetic variability of Phakopsora pachyrhizi accessed by microsatellite markers
Tschurtschenthaler, Nely Norder
Glycine max; genetic diversity; Asian soybean rust; genetic resistance; vertical resistance; SSR
Glycine max; diversidade genética; ferrugem‑asiática‑da‑soja; resistência genética; resistência vertical; SSR
title_short Genetic variability of Phakopsora pachyrhizi accessed by microsatellite markers
title_full Genetic variability of Phakopsora pachyrhizi accessed by microsatellite markers
title_fullStr Genetic variability of Phakopsora pachyrhizi accessed by microsatellite markers
title_full_unstemmed Genetic variability of Phakopsora pachyrhizi accessed by microsatellite markers
title_sort Genetic variability of Phakopsora pachyrhizi accessed by microsatellite markers
author Tschurtschenthaler, Nely Norder
author_facet Tschurtschenthaler, Nely Norder
Vieira, Elisa Serra Negra
Dalla Nora, Tatiane
Schuster, Ivan
author_role author
author2 Vieira, Elisa Serra Negra
Dalla Nora, Tatiane
Schuster, Ivan
author2_role author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Tschurtschenthaler, Nely Norder
Vieira, Elisa Serra Negra
Dalla Nora, Tatiane
Schuster, Ivan
dc.subject.por.fl_str_mv Glycine max; genetic diversity; Asian soybean rust; genetic resistance; vertical resistance; SSR
Glycine max; diversidade genética; ferrugem‑asiática‑da‑soja; resistência genética; resistência vertical; SSR
topic Glycine max; genetic diversity; Asian soybean rust; genetic resistance; vertical resistance; SSR
Glycine max; diversidade genética; ferrugem‑asiática‑da‑soja; resistência genética; resistência vertical; SSR
description The objective of this work was to evaluate the genetic variability of Asian soybean rust in Brazil using microsatellite markers. Samples of Phakopsora pachyrhizi spores collected in the Southern, Southeastern, and Central West regions of Brazil were subjected to analysis of genetic variability, accessed with microsatellite markers specific for the fungus. Spores of the fungus were also collected in several soybean varieties in a same locality, including samples with reddish‑brown (RB) lesions. Among these samples, there was no variability. Tissues with RB lesions did not contain spores of the fungus and did not amplify with the specific markers for P. pachyrhizi. Genetic variability among samples collected in the three regions ranged from 0 to 0.36. A tendency to cluster samples of the Southern and Central West regions of Brazil in different groups was observed. The existence of genetic variability in P. pachyrhizi spore populations indicates that vertical genetic resistance, provided by single genes, is a risky strategy for soybean breeding programs that aim resistance to Asian soybean rust.
publishDate 2012
dc.date.none.fl_str_mv 2012-04-04
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/10933
url https://seer.sct.embrapa.br/index.php/pab/article/view/10933
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/10933/6780
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/10933/6349
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.47, n.2, fev. 2012; 181-186
Pesquisa Agropecuária Brasileira; v.47, n.2, fev. 2012; 181-186
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
_version_ 1793416681646718976

Registros relacionados