Mycorrhizal inoculation and induction of chitinases and β-1,3-glucanases and fusarium resistance in grapevine rootstock
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/7786 |
Resumo: | The objective of this work was to evaluate the expression levels of β-1,3-glucanases and chitinases in SO4 and 110 grapevine rootstocks, respectively susceptible and resistant to Fusarium oxysporum f. sp. herbemontis, as well as to evaluate the effect of the arbuscular mycorrhizal fungus Glomus intraradices on plant growth, on enzyme expression and on pathogen suppression in the susceptible rootstock. The enzyme activities of β-1,3-glucanases and chitinases in the rootstocks roots were evaluated. Plant growth, enzyme activity, and disease symptoms were evaluated in SO4 plantlets inoculated with G. intraradices and F. oxysporum. Enzyme activities in the plant roots increased in the resistant rootstock, between 0 and 5 days after pathogen inoculation. In the susceptible rootstock, chitinase activity enhanced after the mycorrhizal fungus and the pathogen inoculations. β-1,3-glucanase activity increased only in the presence of both the mycorrhizal and the pathogenic fungi. G. intraradices-inoculated SO4 grapevine plantlets showed decreased symptoms of Fusarium infection, which indicates that the mycorrhizal fungus promotes chitinase and β-1,3-glucanase activities leading to pathogen suppression or inhibition. |
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Mycorrhizal inoculation and induction of chitinases and β-1,3-glucanases and fusarium resistance in grapevine rootstockMicorrização e indução de quitinases e β-1,3-glucanases e resistência à fusariose em porta-enxerto de videiraFusarium oxysporum; Glomus; VitisFusarium oxysporum; Glomus; VitisThe objective of this work was to evaluate the expression levels of β-1,3-glucanases and chitinases in SO4 and 110 grapevine rootstocks, respectively susceptible and resistant to Fusarium oxysporum f. sp. herbemontis, as well as to evaluate the effect of the arbuscular mycorrhizal fungus Glomus intraradices on plant growth, on enzyme expression and on pathogen suppression in the susceptible rootstock. The enzyme activities of β-1,3-glucanases and chitinases in the rootstocks roots were evaluated. Plant growth, enzyme activity, and disease symptoms were evaluated in SO4 plantlets inoculated with G. intraradices and F. oxysporum. Enzyme activities in the plant roots increased in the resistant rootstock, between 0 and 5 days after pathogen inoculation. In the susceptible rootstock, chitinase activity enhanced after the mycorrhizal fungus and the pathogen inoculations. β-1,3-glucanase activity increased only in the presence of both the mycorrhizal and the pathogenic fungi. G. intraradices-inoculated SO4 grapevine plantlets showed decreased symptoms of Fusarium infection, which indicates that the mycorrhizal fungus promotes chitinase and β-1,3-glucanase activities leading to pathogen suppression or inhibition.O objetivo deste trabalho foi avaliar os níveis de expressão de β-1,3-glucanases e quitinases nos porta-enxertos de videira SO4 e R110, respectivamente suscetível e resistente a Fusarium oxysporum f. sp. herbemontis, bem como avaliar o efeito do fungo micorrízico arbuscular Glomus intraradices no crescimento, na expressão dessas enzimas e na supressão do patógeno no porta-enxerto suscetível. Foram quantificadas as atividades enzimáticas de β-1,3-glucanases e quitinases nas raízes dos porta-enxertos. Mudas do porta-enxerto SO4 receberam inóculos de G. intraradices e F. oxysporum, e foram avaliadas quanto ao crescimento, atividade das duas enzimas e sintomas de doença. As atividades das enzimas nas raízes do porta-enxerto resistente aumentaram entre 0 e 5 dias após a inoculação do patógeno. A atividade de quitinases nas raízes do porta-enxerto suscetível aumentou com a inoculação do fungo micorrízico e do patógeno. A atividade de β-1,3-glucanases foi maior somente com a presença do fungo micorrízico e do patógeno. Videiras com inoculação de G. intraradices apresentaram diminuição nos sintomas de infecção por Fusarium spp., o que indica que o fungo micorrízico promove a indução de quitinases e β-1,3-glucanases especificamente na supressão ou inibição do patógeno.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCAPESCosta, Murilo DallaLovato, Paulo EmílioSete, Paula Beatriz2011-01-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/7786Pesquisa Agropecuaria Brasileira; v.45, n.4, abr. 2010; 376-383Pesquisa Agropecuária Brasileira; v.45, n.4, abr. 2010; 376-3831678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/7786/5976https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/7786/3377info:eu-repo/semantics/openAccess2014-10-17T17:13:20Zoai:ojs.seer.sct.embrapa.br:article/7786Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-10-17T17:13:20Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Mycorrhizal inoculation and induction of chitinases and β-1,3-glucanases and fusarium resistance in grapevine rootstock Micorrização e indução de quitinases e β-1,3-glucanases e resistência à fusariose em porta-enxerto de videira |
title |
Mycorrhizal inoculation and induction of chitinases and β-1,3-glucanases and fusarium resistance in grapevine rootstock |
spellingShingle |
Mycorrhizal inoculation and induction of chitinases and β-1,3-glucanases and fusarium resistance in grapevine rootstock Costa, Murilo Dalla Fusarium oxysporum; Glomus; Vitis Fusarium oxysporum; Glomus; Vitis |
title_short |
Mycorrhizal inoculation and induction of chitinases and β-1,3-glucanases and fusarium resistance in grapevine rootstock |
title_full |
Mycorrhizal inoculation and induction of chitinases and β-1,3-glucanases and fusarium resistance in grapevine rootstock |
title_fullStr |
Mycorrhizal inoculation and induction of chitinases and β-1,3-glucanases and fusarium resistance in grapevine rootstock |
title_full_unstemmed |
Mycorrhizal inoculation and induction of chitinases and β-1,3-glucanases and fusarium resistance in grapevine rootstock |
title_sort |
Mycorrhizal inoculation and induction of chitinases and β-1,3-glucanases and fusarium resistance in grapevine rootstock |
author |
Costa, Murilo Dalla |
author_facet |
Costa, Murilo Dalla Lovato, Paulo Emílio Sete, Paula Beatriz |
author_role |
author |
author2 |
Lovato, Paulo Emílio Sete, Paula Beatriz |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
CAPES |
dc.contributor.author.fl_str_mv |
Costa, Murilo Dalla Lovato, Paulo Emílio Sete, Paula Beatriz |
dc.subject.por.fl_str_mv |
Fusarium oxysporum; Glomus; Vitis Fusarium oxysporum; Glomus; Vitis |
topic |
Fusarium oxysporum; Glomus; Vitis Fusarium oxysporum; Glomus; Vitis |
description |
The objective of this work was to evaluate the expression levels of β-1,3-glucanases and chitinases in SO4 and 110 grapevine rootstocks, respectively susceptible and resistant to Fusarium oxysporum f. sp. herbemontis, as well as to evaluate the effect of the arbuscular mycorrhizal fungus Glomus intraradices on plant growth, on enzyme expression and on pathogen suppression in the susceptible rootstock. The enzyme activities of β-1,3-glucanases and chitinases in the rootstocks roots were evaluated. Plant growth, enzyme activity, and disease symptoms were evaluated in SO4 plantlets inoculated with G. intraradices and F. oxysporum. Enzyme activities in the plant roots increased in the resistant rootstock, between 0 and 5 days after pathogen inoculation. In the susceptible rootstock, chitinase activity enhanced after the mycorrhizal fungus and the pathogen inoculations. β-1,3-glucanase activity increased only in the presence of both the mycorrhizal and the pathogenic fungi. G. intraradices-inoculated SO4 grapevine plantlets showed decreased symptoms of Fusarium infection, which indicates that the mycorrhizal fungus promotes chitinase and β-1,3-glucanase activities leading to pathogen suppression or inhibition. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-18 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/7786 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/7786 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/7786/5976 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/7786/3377 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.45, n.4, abr. 2010; 376-383 Pesquisa Agropecuária Brasileira; v.45, n.4, abr. 2010; 376-383 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416681636233216 |