PCR detection of Clostridium chauvoei in tissues of Cavia porcellus

Detalhes bibliográficos
Autor(a) principal: Assis, Ronnie Antunes de
Data de Publicação: 2005
Outros Autores: Lobato, Francisco Carlos Faria, Miyakawa, Mariano Fernandez, Uzal, Francisco Alejandro
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/7092
Resumo: The objective of this work was the standardization of a polymerase chain reaction (PCR) for detection of Clostridium chauvoei in formalin-fixed, paraffin-embedded tissues of guinea-pigs (Cavia porcellus) infected experimentally with this microorganism. The animals were sacrificed, and samples of muscle from inoculation area (MIA), liver, myocardium and spleen were available for PCR technique. Clostridium chauvoei was detected in all sections of the MIA, liver and myocardium, and no product was observed in sections of the spleen. Cross-reactions were not observed in sections of MIA of the animals inoculated with other clostridia, as well as no amplification was observed in sections of MIA of control animals. These results show that the PCR technique developed in this study may be useful for detection of C. chauvoei in formalin-fixed, paraffin-embedded tissues.
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spelling PCR detection of Clostridium chauvoei in tissues of Cavia porcellusReação em cadeia da polimerase para detecção de Clostridium chauvoei em tecidos de Cavia porcellusblackleg; malignant edemacarbúnculo sintomático; edema malignoThe objective of this work was the standardization of a polymerase chain reaction (PCR) for detection of Clostridium chauvoei in formalin-fixed, paraffin-embedded tissues of guinea-pigs (Cavia porcellus) infected experimentally with this microorganism. The animals were sacrificed, and samples of muscle from inoculation area (MIA), liver, myocardium and spleen were available for PCR technique. Clostridium chauvoei was detected in all sections of the MIA, liver and myocardium, and no product was observed in sections of the spleen. Cross-reactions were not observed in sections of MIA of the animals inoculated with other clostridia, as well as no amplification was observed in sections of MIA of control animals. These results show that the PCR technique developed in this study may be useful for detection of C. chauvoei in formalin-fixed, paraffin-embedded tissues.O objetivo deste trabalho foi padronizar uma técnica de reação em cadeia da polimerase (PCR), para detecção de Clostridium chauvoei, em tecidos fixados em formol e incluídos em parafina de cobaias (Cavia porcellus) experimentalmente infectadas com esse microrganismo. Os animais foram sacrificados, e amostras do músculo da área de inoculação (MAI), fígado, miocárdio e baço foram disponibilizadas para a técnica de PCR. O clostrídio foi detectado em todas as secções do MAI, fígado e miocárdio, mas não foi observado em secções do baço. Reações cruzadas não foram observadas a partir de secções do MAI dos animais com inoculação de outras espécies de clostrídios, bem como nenhuma amplificação foi observada a partir de secções do MAI dos animais controle. Esses resultados mostram que a técnica de PCR desenvolvida neste estudo, pode ser usada para detecção de Clostridium chauvoei em tecidos fixados em formol e incluídos em parafina.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraAssis, Ronnie Antunes deLobato, Francisco Carlos FariaMiyakawa, Mariano FernandezUzal, Francisco Alejandro2005-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/7092Pesquisa Agropecuaria Brasileira; v.40, n.12, dez. 2005; 1247-1250Pesquisa Agropecuária Brasileira; v.40, n.12, dez. 2005; 1247-12501678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/7092/4140info:eu-repo/semantics/openAccess2010-10-07T14:54:29Zoai:ojs.seer.sct.embrapa.br:article/7092Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2010-10-07T14:54:29Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv PCR detection of Clostridium chauvoei in tissues of Cavia porcellus
Reação em cadeia da polimerase para detecção de Clostridium chauvoei em tecidos de Cavia porcellus
title PCR detection of Clostridium chauvoei in tissues of Cavia porcellus
spellingShingle PCR detection of Clostridium chauvoei in tissues of Cavia porcellus
Assis, Ronnie Antunes de
blackleg; malignant edema
carbúnculo sintomático; edema maligno
title_short PCR detection of Clostridium chauvoei in tissues of Cavia porcellus
title_full PCR detection of Clostridium chauvoei in tissues of Cavia porcellus
title_fullStr PCR detection of Clostridium chauvoei in tissues of Cavia porcellus
title_full_unstemmed PCR detection of Clostridium chauvoei in tissues of Cavia porcellus
title_sort PCR detection of Clostridium chauvoei in tissues of Cavia porcellus
author Assis, Ronnie Antunes de
author_facet Assis, Ronnie Antunes de
Lobato, Francisco Carlos Faria
Miyakawa, Mariano Fernandez
Uzal, Francisco Alejandro
author_role author
author2 Lobato, Francisco Carlos Faria
Miyakawa, Mariano Fernandez
Uzal, Francisco Alejandro
author2_role author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Assis, Ronnie Antunes de
Lobato, Francisco Carlos Faria
Miyakawa, Mariano Fernandez
Uzal, Francisco Alejandro
dc.subject.por.fl_str_mv blackleg; malignant edema
carbúnculo sintomático; edema maligno
topic blackleg; malignant edema
carbúnculo sintomático; edema maligno
description The objective of this work was the standardization of a polymerase chain reaction (PCR) for detection of Clostridium chauvoei in formalin-fixed, paraffin-embedded tissues of guinea-pigs (Cavia porcellus) infected experimentally with this microorganism. The animals were sacrificed, and samples of muscle from inoculation area (MIA), liver, myocardium and spleen were available for PCR technique. Clostridium chauvoei was detected in all sections of the MIA, liver and myocardium, and no product was observed in sections of the spleen. Cross-reactions were not observed in sections of MIA of the animals inoculated with other clostridia, as well as no amplification was observed in sections of MIA of control animals. These results show that the PCR technique developed in this study may be useful for detection of C. chauvoei in formalin-fixed, paraffin-embedded tissues.
publishDate 2005
dc.date.none.fl_str_mv 2005-12-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7092
url https://seer.sct.embrapa.br/index.php/pab/article/view/7092
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7092/4140
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.40, n.12, dez. 2005; 1247-1250
Pesquisa Agropecuária Brasileira; v.40, n.12, dez. 2005; 1247-1250
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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