Rescue of a non-viable accession and RAPD analysis of recovered plants of Arachis retusa

Detalhes bibliográficos
Autor(a) principal: Gagliardi, Rachel Fatima
Data de Publicação: 2004
Outros Autores: Pacheco, Georgia Pereira, Oliveira, Carlos Alberto, Caneiro, Leonardo Alves, Valls, José Francisco Montenegro, Vieira, Maria Lucia Caneiro, Mansur, Elisabeth
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/6757
Resumo: In vitro regeneration of Arachis retusa was examined for the purpose of germplasm renewal and conservation. Random amplified polymorphic DNA (RAPD) fingerprinting was used to evaluate the genetic stability of plants derived from embryo axes and apical segments. Ten arbitrary decamer primers were screened and five of them were selected. Ninety genomic regions were evaluated, with an average of 18 loci per clone. All amplified segments were monomorphic. The results indicate that recovered plants are genetically stable at the assessed genomic regions and that both regeneration processes are suitable for in vitro germplasm preservation of Arachis species.
id EMBRAPA-4_96df5f578396aed2b3cd4fa8f31ffdc6
oai_identifier_str oai:ojs.seer.sct.embrapa.br:article/6757
network_acronym_str EMBRAPA-4
network_name_str Pesquisa Agropecuária Brasileira (Online)
repository_id_str
spelling Rescue of a non-viable accession and RAPD analysis of recovered plants of Arachis retusaResgate de um acesso inviável de Arachis retusa e análise das plantas recuperadas por RAPDgermplasm conservation; micropropagation; molecular characterization; genetic stabilityconservação de germoplasma; micropropagação; caracterização molecular; estabilidade genéticaIn vitro regeneration of Arachis retusa was examined for the purpose of germplasm renewal and conservation. Random amplified polymorphic DNA (RAPD) fingerprinting was used to evaluate the genetic stability of plants derived from embryo axes and apical segments. Ten arbitrary decamer primers were screened and five of them were selected. Ninety genomic regions were evaluated, with an average of 18 loci per clone. All amplified segments were monomorphic. The results indicate that recovered plants are genetically stable at the assessed genomic regions and that both regeneration processes are suitable for in vitro germplasm preservation of Arachis species.A regeneração in vitro de Arachis retusa foi avaliada visando à renovação e conservação de germoplasma. A estabilidade genética de plantas derivadas de eixos embrionários e segmentos apicais foi avaliada por RAPD. Foram analisados dez oligonucleotídeos decâmeros arbitrários, dos quais cinco foram selecionados. Noventa regiões genômicas foram avaliadas, com uma média de 18 loci por clone. Todos os segmentos amplificados foram monomórficos. Estes resultados indicam que as plantas são geneticamente estáveis nas regiões genômicas examinadas e que ambos os processos são adequados para a conservação in vitro do germoplasma de Arachis.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraGagliardi, Rachel FatimaPacheco, Georgia PereiraOliveira, Carlos AlbertoCaneiro, Leonardo AlvesValls, José Francisco MontenegroVieira, Maria Lucia CaneiroMansur, Elisabeth2004-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/6757Pesquisa Agropecuaria Brasileira; v.39, n.2, fev. 2004; 197-199Pesquisa Agropecuária Brasileira; v.39, n.2, fev. 2004; 197-1991678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/6757/3813info:eu-repo/semantics/openAccess2014-07-10T17:52:53Zoai:ojs.seer.sct.embrapa.br:article/6757Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-07-10T17:52:53Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Rescue of a non-viable accession and RAPD analysis of recovered plants of Arachis retusa
Resgate de um acesso inviável de Arachis retusa e análise das plantas recuperadas por RAPD
title Rescue of a non-viable accession and RAPD analysis of recovered plants of Arachis retusa
spellingShingle Rescue of a non-viable accession and RAPD analysis of recovered plants of Arachis retusa
Gagliardi, Rachel Fatima
germplasm conservation; micropropagation; molecular characterization; genetic stability
conservação de germoplasma; micropropagação; caracterização molecular; estabilidade genética
title_short Rescue of a non-viable accession and RAPD analysis of recovered plants of Arachis retusa
title_full Rescue of a non-viable accession and RAPD analysis of recovered plants of Arachis retusa
title_fullStr Rescue of a non-viable accession and RAPD analysis of recovered plants of Arachis retusa
title_full_unstemmed Rescue of a non-viable accession and RAPD analysis of recovered plants of Arachis retusa
title_sort Rescue of a non-viable accession and RAPD analysis of recovered plants of Arachis retusa
author Gagliardi, Rachel Fatima
author_facet Gagliardi, Rachel Fatima
Pacheco, Georgia Pereira
Oliveira, Carlos Alberto
Caneiro, Leonardo Alves
Valls, José Francisco Montenegro
Vieira, Maria Lucia Caneiro
Mansur, Elisabeth
author_role author
author2 Pacheco, Georgia Pereira
Oliveira, Carlos Alberto
Caneiro, Leonardo Alves
Valls, José Francisco Montenegro
Vieira, Maria Lucia Caneiro
Mansur, Elisabeth
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Gagliardi, Rachel Fatima
Pacheco, Georgia Pereira
Oliveira, Carlos Alberto
Caneiro, Leonardo Alves
Valls, José Francisco Montenegro
Vieira, Maria Lucia Caneiro
Mansur, Elisabeth
dc.subject.por.fl_str_mv germplasm conservation; micropropagation; molecular characterization; genetic stability
conservação de germoplasma; micropropagação; caracterização molecular; estabilidade genética
topic germplasm conservation; micropropagation; molecular characterization; genetic stability
conservação de germoplasma; micropropagação; caracterização molecular; estabilidade genética
description In vitro regeneration of Arachis retusa was examined for the purpose of germplasm renewal and conservation. Random amplified polymorphic DNA (RAPD) fingerprinting was used to evaluate the genetic stability of plants derived from embryo axes and apical segments. Ten arbitrary decamer primers were screened and five of them were selected. Ninety genomic regions were evaluated, with an average of 18 loci per clone. All amplified segments were monomorphic. The results indicate that recovered plants are genetically stable at the assessed genomic regions and that both regeneration processes are suitable for in vitro germplasm preservation of Arachis species.
publishDate 2004
dc.date.none.fl_str_mv 2004-02-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6757
url https://seer.sct.embrapa.br/index.php/pab/article/view/6757
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6757/3813
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.39, n.2, fev. 2004; 197-199
Pesquisa Agropecuária Brasileira; v.39, n.2, fev. 2004; 197-199
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
_version_ 1793416682329341952

Registros relacionados