Viability of dourado embryos cooled in different cryoprotectant solutions

• Autor(a) principal: Fornari, Darci Carlos
• Data de Publicação: 2013
• Outros Autores: Streit Junior, Danilo Pedro, Galo, Juliana Minardi, Guerreiro, Luís Jayme, Povh, Jayme Aparecido, Barrero, Nelson Mauricio Lopera, Oliveira, Diego de, Ribeiro, Ricardo Pereira
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Pesquisa Agropecuária Brasileira (Online)
• Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/11186
• topic: Salminus brasiliensis; cryopreservation; fish embryo; embryo preservation; larvae eclosion; Salminus brasiliensis; criopreservação; embrião de peixe; preservação de embriões; eclosão de larvas

The objective of this work was to evaluate the effect of different cryoprotectants on the viability of dourado (Salminus brasiliensis) embryos. Ten cryoprotectant solutions were tested. For each solution, 300 embryos were selected at the closing of the blastopore stage, and 300 more embryos were used as a negative control. After cooling (‑8°C for 6 hours), the embryos were rehydrated directly in the incubator until hatching. The best result is obtained with the cryoprotectant solution containing 9% methanol associated with 17% sucrose, resulting in a larvae hatching rate of 67.06%.