New genes conferring resistance to Asian soybean rust: allelic testing for the Rpp2 and Rpp4 loci

Detalhes bibliográficos
Autor(a) principal: Laperuta, Larissa Di Cássia
Data de Publicação: 2009
Outros Autores: Arias, Carlos Alberto Arrabal, Ribeiro, Aliny Simony, Rachid, Breno Francovig, Pierozzi, Pedro Henrique Braga, Toledo, José Francisco Ferraz de, Pípolo, Antonio Eduardo, Carneiro, Geraldo Estevam de Souza
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/1129
Resumo: The objective of this study was to conduct allelic tests including crosses between a group of rust resistant genotypes from Embrapa's soybean germplasm collection and PI 230970 and PI 459025, which carry the Rpp2 and Rpp4 genes, respectively. Asian Soybean rust (ASR) caused by the fungus Phakopsora pachyrhizi has resulted in significant yield losses and concern among Brazilian farmers. Until recently, there were four resistance genes (Rpp1 through Rpp4) described in the literature, but only Rpp2 and Rpp4 are still effective in Brazil. Twenty-six sources of resistance to P. pachyrhizi were crossed with PI 230970 and PI 459025 (Rpp2 and Rpp4 gene sources, respectively) and plants of their F2 generations were infected with a suspension containing 2.5x104 spores per milliliter and assessed in a greenhouse after 20 days, for the presence of resistant (RB) or susceptible (TAN) lesions. Chi-square tests were applied to investigate the hypotheses of independent or allelic resistance gene segregations. ASR resistant genes derived from PI 197182, PI 230971 and PI 417125 did not segregate in crosses with PI 230970, which indicates that these genotypes have a single resistance gene in the Rpp2 locus. Crosses with the other 23 genotypes resulted in segregating populations, suggesting that their resistance genes do not belong to Rpp2 or Rpp4 loci.
id EMBRAPA-4_9c3dc30ec88ac34d2a151254a3259b59
oai_identifier_str oai:ojs.seer.sct.embrapa.br:article/1129
network_acronym_str EMBRAPA-4
network_name_str Pesquisa Agropecuária Brasileira (Online)
repository_id_str
spelling New genes conferring resistance to Asian soybean rust: allelic testing for the Rpp2 and Rpp4 lociNovos genes de resistência à ferrugem-asiática-da-soja: teste de alelismo para os locos Rpp2 e Rpp4Glycine max; Phakopsora pachyrhizi; inheritance; vertical resistanceGlycine max; Phakopsora pachyrhizi; herança; resistência verticalThe objective of this study was to conduct allelic tests including crosses between a group of rust resistant genotypes from Embrapa's soybean germplasm collection and PI 230970 and PI 459025, which carry the Rpp2 and Rpp4 genes, respectively. Asian Soybean rust (ASR) caused by the fungus Phakopsora pachyrhizi has resulted in significant yield losses and concern among Brazilian farmers. Until recently, there were four resistance genes (Rpp1 through Rpp4) described in the literature, but only Rpp2 and Rpp4 are still effective in Brazil. Twenty-six sources of resistance to P. pachyrhizi were crossed with PI 230970 and PI 459025 (Rpp2 and Rpp4 gene sources, respectively) and plants of their F2 generations were infected with a suspension containing 2.5x104 spores per milliliter and assessed in a greenhouse after 20 days, for the presence of resistant (RB) or susceptible (TAN) lesions. Chi-square tests were applied to investigate the hypotheses of independent or allelic resistance gene segregations. ASR resistant genes derived from PI 197182, PI 230971 and PI 417125 did not segregate in crosses with PI 230970, which indicates that these genotypes have a single resistance gene in the Rpp2 locus. Crosses with the other 23 genotypes resulted in segregating populations, suggesting that their resistance genes do not belong to Rpp2 or Rpp4 loci.O objetivo deste estudo foi desenvolver testes de alelismo, inclusive cruzamentos entre um grupo de genótipos resistentes à ferrugem, do banco de germoplasma de soja da Embrapa, e as duas fontes de resistência com os genes Rpp2 e Rpp4. A ferrugem-asiática-da-soja, causada pelo fungo Phakopsora pachyrhizi, tem gerado perdas significativas na produtividade da cultura e preocupado os agricultores brasileiros. Até recentemente, existiam quatro genes de resistência descritos na literatura, mas somente Rpp2 e Rpp4 continuam efetivos no Brasil. Vinte e seis fontes com resistência a P. pachyrhizi foram cruzadas com PI 230970 e PI 459025 (fontes dos genes Rpp2 e Rpp4, respectivamente), e as plantas da geração F2 foram infectadas com uma suspensão com 2,5x104 esporos por mililitro e analisadas em casa de vegetação após 20 dias, para verificação da presença de lesões de resistência (RB) ou de suscetibilidade (TAN). Foram aplicados testes de qui-quadrado para investigar as hipóteses de segregação independente ou de alelos de resistência nos locos Rpp2 e Rpp4. Genes de resistência provenientes de PI 197182, PI 230971 e PI 417125 não segregaram em cruzamentos com a PI 230970, o que indica que esses genótipos apresentam um único gene de resistência presente no loco Rpp2. Cruzamentos com os outros 23 genótipos resultaram em populações segregantes, o que indica que estas possuem genes que não pertencem aos locos Rpp2 e Rpp4.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraEmbrapa Soja e CNPqLaperuta, Larissa Di CássiaArias, Carlos Alberto ArrabalRibeiro, Aliny SimonyRachid, Breno FrancovigPierozzi, Pedro Henrique BragaToledo, José Francisco Ferraz dePípolo, Antonio EduardoCarneiro, Geraldo Estevam de Souza2009-01-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/1129Pesquisa Agropecuaria Brasileira; v.43, n.12, dez. 2008; 1741-1747Pesquisa Agropecuária Brasileira; v.43, n.12, dez. 2008; 1741-17471678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/1129/5586https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/1129/850info:eu-repo/semantics/openAccess2012-06-17T11:45:37Zoai:ojs.seer.sct.embrapa.br:article/1129Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2012-06-17T11:45:37Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv New genes conferring resistance to Asian soybean rust: allelic testing for the Rpp2 and Rpp4 loci
Novos genes de resistência à ferrugem-asiática-da-soja: teste de alelismo para os locos Rpp2 e Rpp4
title New genes conferring resistance to Asian soybean rust: allelic testing for the Rpp2 and Rpp4 loci
spellingShingle New genes conferring resistance to Asian soybean rust: allelic testing for the Rpp2 and Rpp4 loci
Laperuta, Larissa Di Cássia
Glycine max; Phakopsora pachyrhizi; inheritance; vertical resistance
Glycine max; Phakopsora pachyrhizi; herança; resistência vertical
title_short New genes conferring resistance to Asian soybean rust: allelic testing for the Rpp2 and Rpp4 loci
title_full New genes conferring resistance to Asian soybean rust: allelic testing for the Rpp2 and Rpp4 loci
title_fullStr New genes conferring resistance to Asian soybean rust: allelic testing for the Rpp2 and Rpp4 loci
title_full_unstemmed New genes conferring resistance to Asian soybean rust: allelic testing for the Rpp2 and Rpp4 loci
title_sort New genes conferring resistance to Asian soybean rust: allelic testing for the Rpp2 and Rpp4 loci
author Laperuta, Larissa Di Cássia
author_facet Laperuta, Larissa Di Cássia
Arias, Carlos Alberto Arrabal
Ribeiro, Aliny Simony
Rachid, Breno Francovig
Pierozzi, Pedro Henrique Braga
Toledo, José Francisco Ferraz de
Pípolo, Antonio Eduardo
Carneiro, Geraldo Estevam de Souza
author_role author
author2 Arias, Carlos Alberto Arrabal
Ribeiro, Aliny Simony
Rachid, Breno Francovig
Pierozzi, Pedro Henrique Braga
Toledo, José Francisco Ferraz de
Pípolo, Antonio Eduardo
Carneiro, Geraldo Estevam de Souza
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv
Embrapa Soja e CNPq
dc.contributor.author.fl_str_mv Laperuta, Larissa Di Cássia
Arias, Carlos Alberto Arrabal
Ribeiro, Aliny Simony
Rachid, Breno Francovig
Pierozzi, Pedro Henrique Braga
Toledo, José Francisco Ferraz de
Pípolo, Antonio Eduardo
Carneiro, Geraldo Estevam de Souza
dc.subject.por.fl_str_mv Glycine max; Phakopsora pachyrhizi; inheritance; vertical resistance
Glycine max; Phakopsora pachyrhizi; herança; resistência vertical
topic Glycine max; Phakopsora pachyrhizi; inheritance; vertical resistance
Glycine max; Phakopsora pachyrhizi; herança; resistência vertical
description The objective of this study was to conduct allelic tests including crosses between a group of rust resistant genotypes from Embrapa's soybean germplasm collection and PI 230970 and PI 459025, which carry the Rpp2 and Rpp4 genes, respectively. Asian Soybean rust (ASR) caused by the fungus Phakopsora pachyrhizi has resulted in significant yield losses and concern among Brazilian farmers. Until recently, there were four resistance genes (Rpp1 through Rpp4) described in the literature, but only Rpp2 and Rpp4 are still effective in Brazil. Twenty-six sources of resistance to P. pachyrhizi were crossed with PI 230970 and PI 459025 (Rpp2 and Rpp4 gene sources, respectively) and plants of their F2 generations were infected with a suspension containing 2.5x104 spores per milliliter and assessed in a greenhouse after 20 days, for the presence of resistant (RB) or susceptible (TAN) lesions. Chi-square tests were applied to investigate the hypotheses of independent or allelic resistance gene segregations. ASR resistant genes derived from PI 197182, PI 230971 and PI 417125 did not segregate in crosses with PI 230970, which indicates that these genotypes have a single resistance gene in the Rpp2 locus. Crosses with the other 23 genotypes resulted in segregating populations, suggesting that their resistance genes do not belong to Rpp2 or Rpp4 loci.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-06
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/1129
url https://seer.sct.embrapa.br/index.php/pab/article/view/1129
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/1129/5586
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/1129/850
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.43, n.12, dez. 2008; 1741-1747
Pesquisa Agropecuária Brasileira; v.43, n.12, dez. 2008; 1741-1747
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
_version_ 1793416693863677952

Registros relacionados