Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance

Detalhes bibliográficos
Autor(a) principal: Pinto, Fernanda de Oliveira
Data de Publicação: 2007
Outros Autores: Maluf, Mirian Perez, Filho, Oliveiro Guerreiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/7578
Resumo: The objective of this work was to identify expressed simple sequence repeats (SSR) markers associated to leaf miner resistance in coffee progenies. Identification of SSR markers was accomplished by directed searches on the Brazilian Coffee Expressed Sequence Tags (EST) database. Sequence analysis of 32 selected SSR loci showed that 65% repeats are of tetra-, 21% of tri- and 14% of dinucleotides. Also, expressed SSR are localized frequently in the 5'–UTR of gene transcript. Moreover, most of the genes containing SSR are associated with defense mechanisms. Polymorphisms were analyzed in progenies segregating for resistance to the leaf miner and corresponding to advanced generations of a Coffea arabica x Coffea racemosa hybrid. Frequency of SSR alleles was 2.1 per locus. However, no polymorphism associated with leaf miner resistance was identified. These results suggest that marker-assisted selection in coffee breeding should be performed on the initial cross, in which genetic variability is still significant.
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spelling Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistanceEstudo de marcadores microssatélites em seqüências expressas de café associadas à resistência ao bicho-mineiroLeucoptera coffeella; microsatellites; molecular markersLeucoptera coffeella; microssatélites; marcadores molecularesThe objective of this work was to identify expressed simple sequence repeats (SSR) markers associated to leaf miner resistance in coffee progenies. Identification of SSR markers was accomplished by directed searches on the Brazilian Coffee Expressed Sequence Tags (EST) database. Sequence analysis of 32 selected SSR loci showed that 65% repeats are of tetra-, 21% of tri- and 14% of dinucleotides. Also, expressed SSR are localized frequently in the 5'–UTR of gene transcript. Moreover, most of the genes containing SSR are associated with defense mechanisms. Polymorphisms were analyzed in progenies segregating for resistance to the leaf miner and corresponding to advanced generations of a Coffea arabica x Coffea racemosa hybrid. Frequency of SSR alleles was 2.1 per locus. However, no polymorphism associated with leaf miner resistance was identified. These results suggest that marker-assisted selection in coffee breeding should be performed on the initial cross, in which genetic variability is still significant.O objetivo deste trabalho foi identificar marcadores do tipo microssatélite (SSR) associados à resistência ao bicho-mineiro. A identificação dos marcadores foi feita por meio de buscas dirigidas no banco de seqüências expressas (EST) de café. Foram selecionadas 32 sequências microssatélites expressas em folhas e relacionadas a mecanismos de defesa. Os locos SSR avaliados apresentaram, em sua maioria, repetições mais complexas, com: 65% das repetições de tetranucleotídeos, 21% de trinucleotídeos e 14% de dinucleotídeos. Os locos avaliados estão freqüentemente localizados na região 5'-UTR do gene e envolvidos com mecanismos de defesa. Polimorfismos foram avaliados em progênies avançadas de cruzamentos entre Coffea racemosa x Coffea arabica, segregantes para resistência ao bicho-mineiro. Destes 32 oligonucleotídeos, 29 amplificaram os locos SSR esperados, e o número médio de alelos por loco foi de 2,1. Não foram identificados alelos polimórficos associados à resistência ao bicho-mineiro. Esses resultados sugerem que a seleção assistida por marcadores, em café, deve ser realizada em cruzamentos iniciais, nos quais a variabilidade genética é maior.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraPinto, Fernanda de OliveiraMaluf, Mirian PerezFilho, Oliveiro Guerreiro2007-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/7578Pesquisa Agropecuaria Brasileira; v.42, n.3, mar. 2007; 377-384Pesquisa Agropecuária Brasileira; v.42, n.3, mar. 2007; 377-3841678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/7578/4497info:eu-repo/semantics/openAccess2014-05-28T18:49:31Zoai:ojs.seer.sct.embrapa.br:article/7578Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-05-28T18:49:31Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance
Estudo de marcadores microssatélites em seqüências expressas de café associadas à resistência ao bicho-mineiro
title Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance
spellingShingle Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance
Pinto, Fernanda de Oliveira
Leucoptera coffeella; microsatellites; molecular markers
Leucoptera coffeella; microssatélites; marcadores moleculares
title_short Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance
title_full Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance
title_fullStr Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance
title_full_unstemmed Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance
title_sort Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance
author Pinto, Fernanda de Oliveira
author_facet Pinto, Fernanda de Oliveira
Maluf, Mirian Perez
Filho, Oliveiro Guerreiro
author_role author
author2 Maluf, Mirian Perez
Filho, Oliveiro Guerreiro
author2_role author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Pinto, Fernanda de Oliveira
Maluf, Mirian Perez
Filho, Oliveiro Guerreiro
dc.subject.por.fl_str_mv Leucoptera coffeella; microsatellites; molecular markers
Leucoptera coffeella; microssatélites; marcadores moleculares
topic Leucoptera coffeella; microsatellites; molecular markers
Leucoptera coffeella; microssatélites; marcadores moleculares
description The objective of this work was to identify expressed simple sequence repeats (SSR) markers associated to leaf miner resistance in coffee progenies. Identification of SSR markers was accomplished by directed searches on the Brazilian Coffee Expressed Sequence Tags (EST) database. Sequence analysis of 32 selected SSR loci showed that 65% repeats are of tetra-, 21% of tri- and 14% of dinucleotides. Also, expressed SSR are localized frequently in the 5'–UTR of gene transcript. Moreover, most of the genes containing SSR are associated with defense mechanisms. Polymorphisms were analyzed in progenies segregating for resistance to the leaf miner and corresponding to advanced generations of a Coffea arabica x Coffea racemosa hybrid. Frequency of SSR alleles was 2.1 per locus. However, no polymorphism associated with leaf miner resistance was identified. These results suggest that marker-assisted selection in coffee breeding should be performed on the initial cross, in which genetic variability is still significant.
publishDate 2007
dc.date.none.fl_str_mv 2007-03-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7578
url https://seer.sct.embrapa.br/index.php/pab/article/view/7578
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7578/4497
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.42, n.3, mar. 2007; 377-384
Pesquisa Agropecuária Brasileira; v.42, n.3, mar. 2007; 377-384
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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