Differential gene expression, induced by salicylic acid and Fusarium oxysporum f. sp. lycopersici infection, in tomato

• Autor(a) principal: Amaral, Daniel Oliveira Jordão do
• Data de Publicação: 2008
• Outros Autores: Lima, Marleide Magalhães de Andrade, Resende, Luciane Vilela, Silva, Márcia Vanusa da
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Pesquisa Agropecuária Brasileira (Online)
• Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/565
• topic: Lycopersicon esculentum; cDNA libraries; complementary DNA; gene expression; salicylic acids; suppression subtractive hybridization; Lycopersicon esculentum; bibliotecas de cDNA; DNA complementar; expressão gênica; ácido salicílico; hibridização subtrativa por supressão

The objective of this work was to determine the transcript profi le of tomato plants (Lycopersicon esculentum Mill.), during Fusarium oxysporum f. sp. lycopersici infection and after foliar application of salicylic acid. The suppression subtractive hybridization (SSH) technique was used to generate a cDNA library enriched for transcripts differentially expressed. A total of 307 clones was identifi ed in two subtractive libraries, which allowed the isolation of several defense-related genes that play roles in different mechanisms of plant resistanceto phytopathogens. Genes with unknown roles were also isolated from the two libraries, which indicates the possibility of identifying new genes not yet reported in studies of stress/defense response. The SSH technique is effective for identifi cation of resistance genes activated by salicylic acid and F. oxysporum f. sp. lycopersici infection. Not only the application of this technique enables a cost effective isolation of differentially expressed sequences, but also it allows the identifi cation of novel sequences in tomato from a relative small number ofsequences.