Genetic variation of an infraspecific chayote complex evaluated by isoenzimatic systems
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | spa por |
| Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
| Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/11666 |
Resumo: | The objective of this work was to estimate the degree of genetic variation in an infraspecific complex of Sechium using isoenzymatic systems. For this purpose, 23 loci were codified by 12 isoenzimatic systems and analyzed in starch gels, in 10 individuals from each of the 30 accesions of Sechium (27 cultivated and three wild relatives). The estimation of genetic variation was based on the average number of alleles per locus (NPAL), polymorphism percentage (PP), observed and expected heterozygosity (Ho and He), relative heterozygosity index (RHI) and Shannon´s index (IS). For NPAL and PP, the average for the 30 accesions was 2.03 and 59.8%, respectively. The analysis of Ho and He showed genetic variation in the infraspecific complex of Sechium, with 0.05 and 0.26 average values, respectively. The RHI showed a deficiency of heterozygote individuals (average of ‑0.75). The IS showed a great diversity in the 30 accesions (0.41). The populations with the greatest genetic variations were Negrito, Verde liso, Negro xalapa, Verde espinoso and Negro cónico; with intermediate variation were Castilla blanco, Caldero and Blanco pequeño; and with little variation were Castilla verde, Cambray and wild relatives. |
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Genetic variation of an infraspecific chayote complex evaluated by isoenzimatic systemsVariación genética en chayote (Sechium spp) mediante sistemas isoenzimáticosVariación genética en el complejo infraespecífico de chayote evaluada mediante sistemas isoenzimáticosSechium; genetic diversity; conservation strategies; allele frequencies; heterozygosity index; genetic breedingSechium spp., diversidad genética, frecuencias alélicasSechium, diversidad genética, estrategias de conservación, frecuencias alélicas, índice de heterocigosidad, mejoramiento genéticoThe objective of this work was to estimate the degree of genetic variation in an infraspecific complex of Sechium using isoenzymatic systems. For this purpose, 23 loci were codified by 12 isoenzimatic systems and analyzed in starch gels, in 10 individuals from each of the 30 accesions of Sechium (27 cultivated and three wild relatives). The estimation of genetic variation was based on the average number of alleles per locus (NPAL), polymorphism percentage (PP), observed and expected heterozygosity (Ho and He), relative heterozygosity index (RHI) and Shannon´s index (IS). For NPAL and PP, the average for the 30 accesions was 2.03 and 59.8%, respectively. The analysis of Ho and He showed genetic variation in the infraspecific complex of Sechium, with 0.05 and 0.26 average values, respectively. The RHI showed a deficiency of heterozygote individuals (average of ‑0.75). The IS showed a great diversity in the 30 accesions (0.41). The populations with the greatest genetic variations were Negrito, Verde liso, Negro xalapa, Verde espinoso and Negro cónico; with intermediate variation were Castilla blanco, Caldero and Blanco pequeño; and with little variation were Castilla verde, Cambray and wild relatives.Para estimar el grado de variación genética dentro del complejo infraespecífico de Sechium, se analizaron 23 loci isoenzimáticos codificados por doce sistemas isoenzimáticos, en geles de almidón, en diez individuos de cada una de 30 accesiones (27 cultivadas y tres silvestres). La variación genética se estimó con base en el número promedio de alelos por locus (NPAL), porcentaje de porlimorfismo (PP), heterocigosidad observada y esperada (Ho y HE), índice relativo de heterocigotos (IRH) e índice de Shannon (IS). Los resultados mostraron que para NPAL y PP el promedio para las 30 accesiones fue de 2.03 y 59.8 %, respectivamente. El análisis de Ho y HE reveló que la variación genética en el complejo infraespecífico de Sechium es alta, con valores promedio de 0.05 y 0.26, respectivamente. El IRH reveló una deficiencia de individuos heterocigotos (promedio de 0.75). El IS mostró gran diversidad en las 30 accesiones (0.41). Las poblaciones con mayor diversidad fueron: negrito, verde liso, negro xalapa, verde espinoso y negro cónico; con una variación intermedia fueron: castilla blanco, caldero y blanco pequeño, y con poca variación: castilla verde, castilla blanco, cambray y los parientes silvestres.El objetivo de este trabajo fue estimar el grado de variación genética dentro del complejo infraespecífico de Sechium mediante el uso de sistemas isoenzimáticos. Se analizaron 23 loci codificados por 12 sistemas isoenzimáticos, en geles de almidón, en 10 individuos de cada una de las 30 accesiones (27 cultivadas y tres silvestres). La variación genética se estimó con base en el número promedio de alelos por locus (NPAL), porcentaje de porlimorfismo (PP), heterocigosidad observada y esperada (Ho y He), índice relativo de heterocigosidad (IRH) e índice de Shannon (IS). Para NPAL y PP, el promedio para las 30 accesiones fue de 2,03 y 59,8%, respectivamente. El análisis de Ho y He mostró variación genética en el complejo infraespecífico de Sechium, con valores promedio de 0,05 y 0,26, respectivamente. El IRH mostró una deficiencia de individuos heterocigotos (promedio de ‑0,75). El IS mostró gran diversidad en las 30 accesiones (0,41). Las poblaciones con mayor diversidad fueron Negrito, Verde liso, Negro xalapa, Verde espinoso y Negro cónico; con una variación intermedia fueron Castilla blanco, Caldero y Blanco pequeño; y, con poca variación, Castilla verde, Cambray y los parientes silvestres.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraAvendaño Arrazate, C. H.Cadena Iñiguez, J.Arevalo Galarza, M. L.Cisneros Solano, V. M.Aguirre Medina, J. F.Moreno Pérez, E. del C.Cortes Cruz, M.Castillo Martínez, C. R.Ramírez Vallejo, P.2012-04-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/11666Pesquisa Agropecuaria Brasileira; v.47, n.2, fev. 2012; 244-252Pesquisa Agropecuária Brasileira; v.47, n.2, fev. 2012; 244-2521678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAspaporhttps://seer.sct.embrapa.br/index.php/pab/article/view/11666/6788https://seer.sct.embrapa.br/index.php/pab/article/view/11666/7460https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/11666/7268info:eu-repo/semantics/openAccess2012-06-17T12:19:22Zoai:ojs.seer.sct.embrapa.br:article/11666Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2012-06-17T12:19:22Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
| dc.title.none.fl_str_mv |
Genetic variation of an infraspecific chayote complex evaluated by isoenzimatic systems Variación genética en chayote (Sechium spp) mediante sistemas isoenzimáticos Variación genética en el complejo infraespecífico de chayote evaluada mediante sistemas isoenzimáticos |
| title |
Genetic variation of an infraspecific chayote complex evaluated by isoenzimatic systems |
| spellingShingle |
Genetic variation of an infraspecific chayote complex evaluated by isoenzimatic systems Avendaño Arrazate, C. H. Sechium; genetic diversity; conservation strategies; allele frequencies; heterozygosity index; genetic breeding Sechium spp., diversidad genética, frecuencias alélicas Sechium, diversidad genética, estrategias de conservación, frecuencias alélicas, índice de heterocigosidad, mejoramiento genético |
| title_short |
Genetic variation of an infraspecific chayote complex evaluated by isoenzimatic systems |
| title_full |
Genetic variation of an infraspecific chayote complex evaluated by isoenzimatic systems |
| title_fullStr |
Genetic variation of an infraspecific chayote complex evaluated by isoenzimatic systems |
| title_full_unstemmed |
Genetic variation of an infraspecific chayote complex evaluated by isoenzimatic systems |
| title_sort |
Genetic variation of an infraspecific chayote complex evaluated by isoenzimatic systems |
| author |
Avendaño Arrazate, C. H. |
| author_facet |
Avendaño Arrazate, C. H. Cadena Iñiguez, J. Arevalo Galarza, M. L. Cisneros Solano, V. M. Aguirre Medina, J. F. Moreno Pérez, E. del C. Cortes Cruz, M. Castillo Martínez, C. R. Ramírez Vallejo, P. |
| author_role |
author |
| author2 |
Cadena Iñiguez, J. Arevalo Galarza, M. L. Cisneros Solano, V. M. Aguirre Medina, J. F. Moreno Pérez, E. del C. Cortes Cruz, M. Castillo Martínez, C. R. Ramírez Vallejo, P. |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
|
| dc.contributor.author.fl_str_mv |
Avendaño Arrazate, C. H. Cadena Iñiguez, J. Arevalo Galarza, M. L. Cisneros Solano, V. M. Aguirre Medina, J. F. Moreno Pérez, E. del C. Cortes Cruz, M. Castillo Martínez, C. R. Ramírez Vallejo, P. |
| dc.subject.por.fl_str_mv |
Sechium; genetic diversity; conservation strategies; allele frequencies; heterozygosity index; genetic breeding Sechium spp., diversidad genética, frecuencias alélicas Sechium, diversidad genética, estrategias de conservación, frecuencias alélicas, índice de heterocigosidad, mejoramiento genético |
| topic |
Sechium; genetic diversity; conservation strategies; allele frequencies; heterozygosity index; genetic breeding Sechium spp., diversidad genética, frecuencias alélicas Sechium, diversidad genética, estrategias de conservación, frecuencias alélicas, índice de heterocigosidad, mejoramiento genético |
| description |
The objective of this work was to estimate the degree of genetic variation in an infraspecific complex of Sechium using isoenzymatic systems. For this purpose, 23 loci were codified by 12 isoenzimatic systems and analyzed in starch gels, in 10 individuals from each of the 30 accesions of Sechium (27 cultivated and three wild relatives). The estimation of genetic variation was based on the average number of alleles per locus (NPAL), polymorphism percentage (PP), observed and expected heterozygosity (Ho and He), relative heterozygosity index (RHI) and Shannon´s index (IS). For NPAL and PP, the average for the 30 accesions was 2.03 and 59.8%, respectively. The analysis of Ho and He showed genetic variation in the infraspecific complex of Sechium, with 0.05 and 0.26 average values, respectively. The RHI showed a deficiency of heterozygote individuals (average of ‑0.75). The IS showed a great diversity in the 30 accesions (0.41). The populations with the greatest genetic variations were Negrito, Verde liso, Negro xalapa, Verde espinoso and Negro cónico; with intermediate variation were Castilla blanco, Caldero and Blanco pequeño; and with little variation were Castilla verde, Cambray and wild relatives. |
| publishDate |
2012 |
| dc.date.none.fl_str_mv |
2012-04-09 |
| dc.type.none.fl_str_mv |
|
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://seer.sct.embrapa.br/index.php/pab/article/view/11666 |
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https://seer.sct.embrapa.br/index.php/pab/article/view/11666 |
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spa por |
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spa por |
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https://seer.sct.embrapa.br/index.php/pab/article/view/11666/6788 https://seer.sct.embrapa.br/index.php/pab/article/view/11666/7460 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/11666/7268 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf application/pdf |
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Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
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Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
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Pesquisa Agropecuaria Brasileira; v.47, n.2, fev. 2012; 244-252 Pesquisa Agropecuária Brasileira; v.47, n.2, fev. 2012; 244-252 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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