Potential of antimicrobial volatile organic compounds to control Sclerotinia sclerotiorum in bean seeds
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/9456 |
Resumo: | The objective of this work was to evaluate the potential of an artificial mixture of volatile organic compounds (VOCs), produced by Saccharomyces cerevisiae, to control Sclerotinia sclerotiorum in vitro and in bean seeds. The phytopathogenic fungus was exposed, in polystyrene plates, to an artificial atmosphere containing a mixture of six VOCs formed by alcohols (ethanol, 3‑methyl‑1‑butanol, 2‑methyl‑1‑butanol and phenylethyl alcohol) and esters (ethyl acetate and ethyl octanoate), in the proportions found in the atmosphere naturally produced by yeast. Bean seeds artificially contamined with the pathogen were fumigated with the mixture of VOCs in sealed glass flasks for four and seven days. In the in vitro assays, the compounds 2‑methyl‑1‑butanol and 3‑methyl‑1‑butanol were the most active against S. sclerotiorum, completely inhibiting its mycelial growth at 0.8 µL mL-1, followed by the ethyl acetate, at 1.2 µL mL-1. Bean seeds fumigated with the VOCs at 3.5 µL mL-1 showed a 75% reduction in S. sclerotiorum incidence after four days of fumigation. The VOCs produced by S. cerevisiae have potential to control the pathogen in stored seeds. |
id |
EMBRAPA-4_aec8b67c6a44177fe91cb1e927d03ea6 |
---|---|
oai_identifier_str |
oai:ojs.seer.sct.embrapa.br:article/9456 |
network_acronym_str |
EMBRAPA-4 |
network_name_str |
Pesquisa Agropecuária Brasileira (Online) |
repository_id_str |
|
spelling |
Potential of antimicrobial volatile organic compounds to control Sclerotinia sclerotiorum in bean seedsPotencial de compostos orgânicos voláteis antimicrobianos no controle de Sclerotinia sclerotiorum em sementes de feijãoPhaseolus vulgaris; alternative control; fumigation; white mold; yeastPhaseolus vulgaris; controle alternativo; fumigação; mofo-branco; leveduraThe objective of this work was to evaluate the potential of an artificial mixture of volatile organic compounds (VOCs), produced by Saccharomyces cerevisiae, to control Sclerotinia sclerotiorum in vitro and in bean seeds. The phytopathogenic fungus was exposed, in polystyrene plates, to an artificial atmosphere containing a mixture of six VOCs formed by alcohols (ethanol, 3‑methyl‑1‑butanol, 2‑methyl‑1‑butanol and phenylethyl alcohol) and esters (ethyl acetate and ethyl octanoate), in the proportions found in the atmosphere naturally produced by yeast. Bean seeds artificially contamined with the pathogen were fumigated with the mixture of VOCs in sealed glass flasks for four and seven days. In the in vitro assays, the compounds 2‑methyl‑1‑butanol and 3‑methyl‑1‑butanol were the most active against S. sclerotiorum, completely inhibiting its mycelial growth at 0.8 µL mL-1, followed by the ethyl acetate, at 1.2 µL mL-1. Bean seeds fumigated with the VOCs at 3.5 µL mL-1 showed a 75% reduction in S. sclerotiorum incidence after four days of fumigation. The VOCs produced by S. cerevisiae have potential to control the pathogen in stored seeds.O objetivo deste trabalho foi avaliar o potencial da mistura de compostos orgânicos voláteis (COVs), produzidos por Saccharomyces cerevisiae, no controle de Sclerotinia sclerotiorum in vitro e em sementes de feijão. O fungo fitopatogênico foi exposto, em placas de poliestireno, a uma atmosfera artificial que continha uma mistura de seis COVs, formada por álcoois (etanol, 3‑metil‑1‑butanol, 2‑metil‑1‑butanol e feniletilalcool) e ésteres (acetato de etila e octanoato de etila) nas proporções encontradas na atmosfera naturalmente produzida pela levedura. Sementes de feijão artificialmente contaminadas com o patógeno foram fumigadas com a mistura de COVs em frascos de vidro selados por quatro e sete dias. Nos experimentos in vitro, os compostos 2‑metil‑1‑butanol e 3‑metil‑1‑butanol foram os mais ativos sobre S. sclerotiorum, com inibição completa do crescimento micelial na concentração de 0,8 µL mL-1, seguidos pelo acetato de etila na concentração de 1,2 µL mL-1. As sementes de feijão fumigadas com os COVs na concentração de 3,5 µL mL-1 apresentaram 75% de redução da incidência de S. sclerotiorum após quatro dias de fumigação. Os COVs produzidos por S. cerevisiae possuem potencial de controle do patógeno em sementes armazenadas.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCAPES, CNPqFialho, Maurício Batistade Moraes, Maria Heloisa DuarteTremocoldi, Annelise RobertaPascholati, Sérgio Florentino2011-05-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/9456Pesquisa Agropecuaria Brasileira; v.46, n.2, fev. 2011; 137-142Pesquisa Agropecuária Brasileira; v.46, n.2, fev. 2011; 137-1421678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/9456/6243https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/9456/4687info:eu-repo/semantics/openAccess2014-05-16T19:29:02Zoai:ojs.seer.sct.embrapa.br:article/9456Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-05-16T19:29:02Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Potential of antimicrobial volatile organic compounds to control Sclerotinia sclerotiorum in bean seeds Potencial de compostos orgânicos voláteis antimicrobianos no controle de Sclerotinia sclerotiorum em sementes de feijão |
title |
Potential of antimicrobial volatile organic compounds to control Sclerotinia sclerotiorum in bean seeds |
spellingShingle |
Potential of antimicrobial volatile organic compounds to control Sclerotinia sclerotiorum in bean seeds Fialho, Maurício Batista Phaseolus vulgaris; alternative control; fumigation; white mold; yeast Phaseolus vulgaris; controle alternativo; fumigação; mofo-branco; levedura |
title_short |
Potential of antimicrobial volatile organic compounds to control Sclerotinia sclerotiorum in bean seeds |
title_full |
Potential of antimicrobial volatile organic compounds to control Sclerotinia sclerotiorum in bean seeds |
title_fullStr |
Potential of antimicrobial volatile organic compounds to control Sclerotinia sclerotiorum in bean seeds |
title_full_unstemmed |
Potential of antimicrobial volatile organic compounds to control Sclerotinia sclerotiorum in bean seeds |
title_sort |
Potential of antimicrobial volatile organic compounds to control Sclerotinia sclerotiorum in bean seeds |
author |
Fialho, Maurício Batista |
author_facet |
Fialho, Maurício Batista de Moraes, Maria Heloisa Duarte Tremocoldi, Annelise Roberta Pascholati, Sérgio Florentino |
author_role |
author |
author2 |
de Moraes, Maria Heloisa Duarte Tremocoldi, Annelise Roberta Pascholati, Sérgio Florentino |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
CAPES, CNPq |
dc.contributor.author.fl_str_mv |
Fialho, Maurício Batista de Moraes, Maria Heloisa Duarte Tremocoldi, Annelise Roberta Pascholati, Sérgio Florentino |
dc.subject.por.fl_str_mv |
Phaseolus vulgaris; alternative control; fumigation; white mold; yeast Phaseolus vulgaris; controle alternativo; fumigação; mofo-branco; levedura |
topic |
Phaseolus vulgaris; alternative control; fumigation; white mold; yeast Phaseolus vulgaris; controle alternativo; fumigação; mofo-branco; levedura |
description |
The objective of this work was to evaluate the potential of an artificial mixture of volatile organic compounds (VOCs), produced by Saccharomyces cerevisiae, to control Sclerotinia sclerotiorum in vitro and in bean seeds. The phytopathogenic fungus was exposed, in polystyrene plates, to an artificial atmosphere containing a mixture of six VOCs formed by alcohols (ethanol, 3‑methyl‑1‑butanol, 2‑methyl‑1‑butanol and phenylethyl alcohol) and esters (ethyl acetate and ethyl octanoate), in the proportions found in the atmosphere naturally produced by yeast. Bean seeds artificially contamined with the pathogen were fumigated with the mixture of VOCs in sealed glass flasks for four and seven days. In the in vitro assays, the compounds 2‑methyl‑1‑butanol and 3‑methyl‑1‑butanol were the most active against S. sclerotiorum, completely inhibiting its mycelial growth at 0.8 µL mL-1, followed by the ethyl acetate, at 1.2 µL mL-1. Bean seeds fumigated with the VOCs at 3.5 µL mL-1 showed a 75% reduction in S. sclerotiorum incidence after four days of fumigation. The VOCs produced by S. cerevisiae have potential to control the pathogen in stored seeds. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-05-20 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/9456 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/9456 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/9456/6243 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/9456/4687 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.46, n.2, fev. 2011; 137-142 Pesquisa Agropecuária Brasileira; v.46, n.2, fev. 2011; 137-142 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
_version_ |
1793416688337682432 |