Fusion of protoplasts with irradiated microprotoplasts as a tool for radiation hybrid panel in citrus

Detalhes bibliográficos
Autor(a) principal: Bona, Claudine Maria de
Data de Publicação: 2010
Outros Autores: Stelly, David, Junior, J. Creighton Miller, Louzada, Eliezer Silva
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/3024
Resumo: The objective of this work was to combine asymmetric somatic hybridization (donor-recipient fusion or gamma fusion) to microprotoplast-mediated chromosome transfer, as a tool to be used for chromosome mapping in Citrus. Swinglea glutinosa microprotoplasts were irradiated either with 50, 70, 100 or 200 gamma rays and fused to cv. Ruby Red grapefruit or Murcott tangor protoplasts. Cell colonies were successfully formed and AFLP analyses confirmed presence of S. glutinosa in both 'Murcott' tangor and 'Ruby Red' grapefruit genomes.
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spelling Fusion of protoplasts with irradiated microprotoplasts as a tool for radiation hybrid panel in citrusFusão de protoplastos com microprotoplastos irradiados como ferramenta para painel híbrido de radiação em citrosCitrus; Swinglea glutinosa; asymmetric somatic hybridization; gamma rays; hybrid cell lines; radiation hybrid mappingCitrus; Swinglea glutinosa; hibridação somática assimétrica; raios gama; linhagens de células híbridas; mapeamento de híbridos por radiaçãoThe objective of this work was to combine asymmetric somatic hybridization (donor-recipient fusion or gamma fusion) to microprotoplast-mediated chromosome transfer, as a tool to be used for chromosome mapping in Citrus. Swinglea glutinosa microprotoplasts were irradiated either with 50, 70, 100 or 200 gamma rays and fused to cv. Ruby Red grapefruit or Murcott tangor protoplasts. Cell colonies were successfully formed and AFLP analyses confirmed presence of S. glutinosa in both 'Murcott' tangor and 'Ruby Red' grapefruit genomes.O objetivo deste trabalho foi combinar hibridação somática assimétrica (fusão doador-recipiente ou fusão gama) e transferência cromossômica mediada por microprotoplastos como uma ferramenta para o mapeamento cromossômico em Citrus. Microprotoplastos de Swinglea glutinosa foram irradiados com doses de 50, 70, 100 ou 200 raios gama e fusionados com protoplastos de pomelo cv. Ruby Red ou de tangor Murcott. Houve sucesso na formação de colônias de células, e a análise AFLP confirmou a presença de S. glutinosa nos genomas do tangor 'Murcott' e do pomelo 'Ruby Red'.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCapesCapesBona, Claudine Maria deStelly, DavidJunior, J. Creighton MillerLouzada, Eliezer Silva2010-12-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/3024Pesquisa Agropecuaria Brasileira; v.44, n.12, dez. 2009; 1616-1623Pesquisa Agropecuária Brasileira; v.44, n.12, dez. 2009; 1616-16231678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/3024/5906https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/3024/2551https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/3024/2555https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/3024/2568https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/3024/2582info:eu-repo/semantics/openAccess2012-06-17T11:54:26Zoai:ojs.seer.sct.embrapa.br:article/3024Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2012-06-17T11:54:26Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Fusion of protoplasts with irradiated microprotoplasts as a tool for radiation hybrid panel in citrus
Fusão de protoplastos com microprotoplastos irradiados como ferramenta para painel híbrido de radiação em citros
title Fusion of protoplasts with irradiated microprotoplasts as a tool for radiation hybrid panel in citrus
spellingShingle Fusion of protoplasts with irradiated microprotoplasts as a tool for radiation hybrid panel in citrus
Bona, Claudine Maria de
Citrus; Swinglea glutinosa; asymmetric somatic hybridization; gamma rays; hybrid cell lines; radiation hybrid mapping
Citrus; Swinglea glutinosa; hibridação somática assimétrica; raios gama; linhagens de células híbridas; mapeamento de híbridos por radiação
title_short Fusion of protoplasts with irradiated microprotoplasts as a tool for radiation hybrid panel in citrus
title_full Fusion of protoplasts with irradiated microprotoplasts as a tool for radiation hybrid panel in citrus
title_fullStr Fusion of protoplasts with irradiated microprotoplasts as a tool for radiation hybrid panel in citrus
title_full_unstemmed Fusion of protoplasts with irradiated microprotoplasts as a tool for radiation hybrid panel in citrus
title_sort Fusion of protoplasts with irradiated microprotoplasts as a tool for radiation hybrid panel in citrus
author Bona, Claudine Maria de
author_facet Bona, Claudine Maria de
Stelly, David
Junior, J. Creighton Miller
Louzada, Eliezer Silva
author_role author
author2 Stelly, David
Junior, J. Creighton Miller
Louzada, Eliezer Silva
author2_role author
author
author
dc.contributor.none.fl_str_mv Capes
Capes
dc.contributor.author.fl_str_mv Bona, Claudine Maria de
Stelly, David
Junior, J. Creighton Miller
Louzada, Eliezer Silva
dc.subject.por.fl_str_mv Citrus; Swinglea glutinosa; asymmetric somatic hybridization; gamma rays; hybrid cell lines; radiation hybrid mapping
Citrus; Swinglea glutinosa; hibridação somática assimétrica; raios gama; linhagens de células híbridas; mapeamento de híbridos por radiação
topic Citrus; Swinglea glutinosa; asymmetric somatic hybridization; gamma rays; hybrid cell lines; radiation hybrid mapping
Citrus; Swinglea glutinosa; hibridação somática assimétrica; raios gama; linhagens de células híbridas; mapeamento de híbridos por radiação
description The objective of this work was to combine asymmetric somatic hybridization (donor-recipient fusion or gamma fusion) to microprotoplast-mediated chromosome transfer, as a tool to be used for chromosome mapping in Citrus. Swinglea glutinosa microprotoplasts were irradiated either with 50, 70, 100 or 200 gamma rays and fused to cv. Ruby Red grapefruit or Murcott tangor protoplasts. Cell colonies were successfully formed and AFLP analyses confirmed presence of S. glutinosa in both 'Murcott' tangor and 'Ruby Red' grapefruit genomes.
publishDate 2010
dc.date.none.fl_str_mv 2010-12-22
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/3024
url https://seer.sct.embrapa.br/index.php/pab/article/view/3024
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/3024/5906
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/3024/2551
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/3024/2555
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/3024/2568
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/3024/2582
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.44, n.12, dez. 2009; 1616-1623
Pesquisa Agropecuária Brasileira; v.44, n.12, dez. 2009; 1616-1623
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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