Variability in the Helminthosporium oryzae fungus
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/16171 |
Resumo: | Cultural and pathogenic variation in Helminthosporium oryzae was studied using 10 isolates under greenhouse conditions. In the absence of a known and standard differential set for H. oryzae, 10 cultivars of rice (Oryza sativa L.) Bluebelle, EEA 406, Caloro, BR-IRGA 410, Dawn, Brazos, Stirpe, IRGA 408, Lebonnet and IV-29-4, were used. Pathogenicity tests were conducted on 30-day old plants. The fungus isolates (H-1 to H-10), obtained from leaves, culms, and/or rice seeds collected from different localities were inoculated on the differential set. The cultures were maintained on Potato - Dextrose-Agar + Streptomicin (100 µg/l). Five single monosporic isolates obtained from each one of the three aggressive original isolates (H-1, H-5 and H-8) were tested on the differencial set. The results showed variation in cultural characters between the original isolates and within the more aggressive ones (monosporic). However, differencial interaction in pathogenicity was not observed among tho isolates and cultivars. |
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Variability in the Helminthosporium oryzae fungusVariabilidade do fungo Helminthosporium oryzaerice; brown spot disease; physiological racesarroz; mancha-parda; raças fisiológicasCultural and pathogenic variation in Helminthosporium oryzae was studied using 10 isolates under greenhouse conditions. In the absence of a known and standard differential set for H. oryzae, 10 cultivars of rice (Oryza sativa L.) Bluebelle, EEA 406, Caloro, BR-IRGA 410, Dawn, Brazos, Stirpe, IRGA 408, Lebonnet and IV-29-4, were used. Pathogenicity tests were conducted on 30-day old plants. The fungus isolates (H-1 to H-10), obtained from leaves, culms, and/or rice seeds collected from different localities were inoculated on the differential set. The cultures were maintained on Potato - Dextrose-Agar + Streptomicin (100 µg/l). Five single monosporic isolates obtained from each one of the three aggressive original isolates (H-1, H-5 and H-8) were tested on the differencial set. The results showed variation in cultural characters between the original isolates and within the more aggressive ones (monosporic). However, differencial interaction in pathogenicity was not observed among tho isolates and cultivars.Foi estudado, em casa de vegetação, o comportamento de dez isolados de Helminthosporium oryzae Breda de Haan, com o objetivo de avaliar a variabilidade desse fungo. Na ausência de uma série diferencial conhecida e padronizada para H. oryzae, foram utilizadas as cultivares de arroz (Oryza sativa L.) Bluebelle, EEA 406, Caloro, BR-IRGA 410, Dawn, Brazos, Stirpe, IRGA 408, Lebonnet e IV-29-4, as quais foram inoculadas quando as plantas atingiram a idade de 30 dias após a emergência. Os isolados H-1 a H-10, obtidos de folhas, colmos e sementes de arroz coletadas em diferentes locais, foram inoculados na série diferencial após o seu cultivo em meio de cultura batata-dextrose-agar (BDA) + estreptomicina (100 µg/l). De cada um dos isolados originais que apresentaram maior virulência (H-1, H-5 e H-8), foram feitos cinco isolados monospóricos e novamente inoculados na série diferencial. Os resultados obtidos mostraram que, tanto entre os isolados originais como dentro dos mais virulentos (monospóricos), foram observadas variações em características culturais. Entretanto, não houve interações diferenciais em patogenicidade entre isolados e cultivares.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraSousa, Nára Regina GerviniRibeiro, Alcêu SallaberryGalli, José2014-04-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/16171Pesquisa Agropecuaria Brasileira; v.19, n.11, nov. 1984; 1335-1343Pesquisa Agropecuária Brasileira; v.19, n.11, nov. 1984; 1335-13431678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/16171/10339info:eu-repo/semantics/openAccess2014-04-16T19:12:37Zoai:ojs.seer.sct.embrapa.br:article/16171Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-04-16T19:12:37Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Variability in the Helminthosporium oryzae fungus Variabilidade do fungo Helminthosporium oryzae |
title |
Variability in the Helminthosporium oryzae fungus |
spellingShingle |
Variability in the Helminthosporium oryzae fungus Sousa, Nára Regina Gervini rice; brown spot disease; physiological races arroz; mancha-parda; raças fisiológicas |
title_short |
Variability in the Helminthosporium oryzae fungus |
title_full |
Variability in the Helminthosporium oryzae fungus |
title_fullStr |
Variability in the Helminthosporium oryzae fungus |
title_full_unstemmed |
Variability in the Helminthosporium oryzae fungus |
title_sort |
Variability in the Helminthosporium oryzae fungus |
author |
Sousa, Nára Regina Gervini |
author_facet |
Sousa, Nára Regina Gervini Ribeiro, Alcêu Sallaberry Galli, José |
author_role |
author |
author2 |
Ribeiro, Alcêu Sallaberry Galli, José |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
|
dc.contributor.author.fl_str_mv |
Sousa, Nára Regina Gervini Ribeiro, Alcêu Sallaberry Galli, José |
dc.subject.por.fl_str_mv |
rice; brown spot disease; physiological races arroz; mancha-parda; raças fisiológicas |
topic |
rice; brown spot disease; physiological races arroz; mancha-parda; raças fisiológicas |
description |
Cultural and pathogenic variation in Helminthosporium oryzae was studied using 10 isolates under greenhouse conditions. In the absence of a known and standard differential set for H. oryzae, 10 cultivars of rice (Oryza sativa L.) Bluebelle, EEA 406, Caloro, BR-IRGA 410, Dawn, Brazos, Stirpe, IRGA 408, Lebonnet and IV-29-4, were used. Pathogenicity tests were conducted on 30-day old plants. The fungus isolates (H-1 to H-10), obtained from leaves, culms, and/or rice seeds collected from different localities were inoculated on the differential set. The cultures were maintained on Potato - Dextrose-Agar + Streptomicin (100 µg/l). Five single monosporic isolates obtained from each one of the three aggressive original isolates (H-1, H-5 and H-8) were tested on the differencial set. The results showed variation in cultural characters between the original isolates and within the more aggressive ones (monosporic). However, differencial interaction in pathogenicity was not observed among tho isolates and cultivars. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04-16 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/16171 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/16171 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/16171/10339 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.19, n.11, nov. 1984; 1335-1343 Pesquisa Agropecuária Brasileira; v.19, n.11, nov. 1984; 1335-1343 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416677776424960 |