Cellulolytic activitity of isolated fungi from sugarcane bagasse and decayed wood

Detalhes bibliográficos
Autor(a) principal: Basso, Thalita Peixoto
Data de Publicação: 2011
Outros Autores: Gallo, Cláudio Rosa, Basso, Luiz Carlos
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/8551
Resumo: The objective of this work was to identify fungi isolates from sugarcane bagasse and decayed wood, and to evaluate their cellulolytic activity on sugarcane bagasse. Five isolates were evaluated, as compared to Trichoderma reesei QM9414 and T. reesei RUT C30. The cellulolytic activity was estimated through the hydrolytic capacity of the enzymatic extract of fungi grown on sugarcane bagasse over filter paper (total cellulolytic activity) and sodium carboxymethyl cellulose (endoglucanase activity). The isolates were identified by molecular analysis of 26S rDNA region. Paecilomyces, Aspergillus, Acremonium/Penicillium and Trichoderma genera were identified. Although T. reesei QM9414 showed the highest total cellulolytic activity, some isolates showed higher endoglucanase activities. Biodiversity found in habitats such as sugarcane bagasse can provide strains of cellulolytic fungi with great biotechnological potential.
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spelling Cellulolytic activitity of isolated fungi from sugarcane bagasse and decayed woodAtividade celulolítica de fungos isolados de bagaço de cana-de-açúcar e madeira em decomposiçãoAcremonium; Aspergillus; Paecilomyces; Penicillium; Trichoderma; cellulase; endoglucanaseAcremonium; Aspergillus; Paecilomyces; Penicillium; Trichoderma; celulase; endoglucanaseThe objective of this work was to identify fungi isolates from sugarcane bagasse and decayed wood, and to evaluate their cellulolytic activity on sugarcane bagasse. Five isolates were evaluated, as compared to Trichoderma reesei QM9414 and T. reesei RUT C30. The cellulolytic activity was estimated through the hydrolytic capacity of the enzymatic extract of fungi grown on sugarcane bagasse over filter paper (total cellulolytic activity) and sodium carboxymethyl cellulose (endoglucanase activity). The isolates were identified by molecular analysis of 26S rDNA region. Paecilomyces, Aspergillus, Acremonium/Penicillium and Trichoderma genera were identified. Although T. reesei QM9414 showed the highest total cellulolytic activity, some isolates showed higher endoglucanase activities. Biodiversity found in habitats such as sugarcane bagasse can provide strains of cellulolytic fungi with great biotechnological potential.O objetivo deste trabalho foi identificar isolados de fungos a partir de bagaço de cana‑de‑açúcar e madeira em decomposição e avaliar a sua atividade celulolítica em bagaço de cana. Cinco isolados foram avaliados, tendo-se como referências os fungos Trichoderma reesei QM9414 e T. reesei RUT C30. A atividade celulolítica foi estimada pela capacidade hidrolítica do extrato enzimático dos fungos cultivados em bagaço de cana sobre os substratos papel de filtro (atividade celulolítica total) e carboximetilcelulose sódica (atividade da endoglucanase). Os isolados foram identificados pela análise molecular da região 26S rDNA. Os gêneros Paecilomyces, Aspergillus, Acremonium/Penicillium e Trichoderma foram identificados. Embora T. reesei QM9414 tenha apresentado a mais alta atividade celulolítica total, alguns isolados também apresentaram alta atividade de endoglucanase. A biodiversidade, em nichos como bagaço de cana‑de‑açúcar, pode fornecer linhagens de fungos celulolíticos com grande potencial biotecnológico.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraBasso, Thalita PeixotoGallo, Cláudio RosaBasso, Luiz Carlos2011-01-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/8551Pesquisa Agropecuaria Brasileira; v.45, n.11, nov. 2010; 1282-1289Pesquisa Agropecuária Brasileira; v.45, n.11, nov. 2010; 1282-12891678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/8551/6098https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/8551/4269info:eu-repo/semantics/openAccess2014-11-13T17:39:31Zoai:ojs.seer.sct.embrapa.br:article/8551Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-11-13T17:39:31Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Cellulolytic activitity of isolated fungi from sugarcane bagasse and decayed wood
Atividade celulolítica de fungos isolados de bagaço de cana-de-açúcar e madeira em decomposição
title Cellulolytic activitity of isolated fungi from sugarcane bagasse and decayed wood
spellingShingle Cellulolytic activitity of isolated fungi from sugarcane bagasse and decayed wood
Basso, Thalita Peixoto
Acremonium; Aspergillus; Paecilomyces; Penicillium; Trichoderma; cellulase; endoglucanase
Acremonium; Aspergillus; Paecilomyces; Penicillium; Trichoderma; celulase; endoglucanase
title_short Cellulolytic activitity of isolated fungi from sugarcane bagasse and decayed wood
title_full Cellulolytic activitity of isolated fungi from sugarcane bagasse and decayed wood
title_fullStr Cellulolytic activitity of isolated fungi from sugarcane bagasse and decayed wood
title_full_unstemmed Cellulolytic activitity of isolated fungi from sugarcane bagasse and decayed wood
title_sort Cellulolytic activitity of isolated fungi from sugarcane bagasse and decayed wood
author Basso, Thalita Peixoto
author_facet Basso, Thalita Peixoto
Gallo, Cláudio Rosa
Basso, Luiz Carlos
author_role author
author2 Gallo, Cláudio Rosa
Basso, Luiz Carlos
author2_role author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Basso, Thalita Peixoto
Gallo, Cláudio Rosa
Basso, Luiz Carlos
dc.subject.por.fl_str_mv Acremonium; Aspergillus; Paecilomyces; Penicillium; Trichoderma; cellulase; endoglucanase
Acremonium; Aspergillus; Paecilomyces; Penicillium; Trichoderma; celulase; endoglucanase
topic Acremonium; Aspergillus; Paecilomyces; Penicillium; Trichoderma; cellulase; endoglucanase
Acremonium; Aspergillus; Paecilomyces; Penicillium; Trichoderma; celulase; endoglucanase
description The objective of this work was to identify fungi isolates from sugarcane bagasse and decayed wood, and to evaluate their cellulolytic activity on sugarcane bagasse. Five isolates were evaluated, as compared to Trichoderma reesei QM9414 and T. reesei RUT C30. The cellulolytic activity was estimated through the hydrolytic capacity of the enzymatic extract of fungi grown on sugarcane bagasse over filter paper (total cellulolytic activity) and sodium carboxymethyl cellulose (endoglucanase activity). The isolates were identified by molecular analysis of 26S rDNA region. Paecilomyces, Aspergillus, Acremonium/Penicillium and Trichoderma genera were identified. Although T. reesei QM9414 showed the highest total cellulolytic activity, some isolates showed higher endoglucanase activities. Biodiversity found in habitats such as sugarcane bagasse can provide strains of cellulolytic fungi with great biotechnological potential.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-28
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/8551
url https://seer.sct.embrapa.br/index.php/pab/article/view/8551
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/8551/6098
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/8551/4269
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.45, n.11, nov. 2010; 1282-1289
Pesquisa Agropecuária Brasileira; v.45, n.11, nov. 2010; 1282-1289
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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