Short‑term storage in vitro and large‑scale propagation of grapevine genotypes
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/9869 |
Resumo: | The objective of this work was to evaluate the large‑scale propagation of grapevine genotypes after short‑term storage in vitro. Microshoots from ten grapevine genotypes were used. The following storage temperatures were evaluated: 10, 20, and 25°C. After short‑term storage, the shoots were propagated in up to five successive subcultures, to assess the large‑scale propagation of the germplasm maintained under conditions of minimal growth. The propagated shoots were rooted in different concentrations of indolbutiric acid (IBA) and acclimatized in greenhouse. The best temperature for short‑term storage in vitro and survival of the genotypes was 20°C. In the propagation phase, the highest number of shoots per explant was found in the subcultures 4 and 5, with averages of 4.9 and 4.8 shoots per explant, respectively. In the rooting phase, thebest results for number of roots were obtained using a culture medium supplemented with 0.4 μmol L‑1 of IBA, with an average of three roots per shoot. During the acclimation phase, a survival rate higher than 95% was achieved after 30 days in the greenhouse. Grapevine genotypes maintained for six months in vitro, at 20oC, can be micropropagated in large scale. |
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Short‑term storage in vitro and large‑scale propagation of grapevine genotypesShort‑term storage in vitro and large‑scale propagation of grapevine genotypesVitis; germplasm; ex situ conservation; in vitro conservation; micropropagation; minimal growthVitis; germoplasma; conservação ex situ; conservação in vitro; micropropagação; crescimento mínimoThe objective of this work was to evaluate the large‑scale propagation of grapevine genotypes after short‑term storage in vitro. Microshoots from ten grapevine genotypes were used. The following storage temperatures were evaluated: 10, 20, and 25°C. After short‑term storage, the shoots were propagated in up to five successive subcultures, to assess the large‑scale propagation of the germplasm maintained under conditions of minimal growth. The propagated shoots were rooted in different concentrations of indolbutiric acid (IBA) and acclimatized in greenhouse. The best temperature for short‑term storage in vitro and survival of the genotypes was 20°C. In the propagation phase, the highest number of shoots per explant was found in the subcultures 4 and 5, with averages of 4.9 and 4.8 shoots per explant, respectively. In the rooting phase, thebest results for number of roots were obtained using a culture medium supplemented with 0.4 μmol L‑1 of IBA, with an average of three roots per shoot. During the acclimation phase, a survival rate higher than 95% was achieved after 30 days in the greenhouse. Grapevine genotypes maintained for six months in vitro, at 20oC, can be micropropagated in large scale.The objective of this work was to evaluate the large‑scale propagation of grapevine genotypes after short‑term storage in vitro. Microshoots from ten grapevine genotypes were used. The following storage temperatures were evaluated: 10, 20, and 25°C. After short‑term storage, the shoots were propagated in up to five successive subcultures, to assess the large‑scale propagation of the germplasm maintained under conditions of minimal growth. The propagated shoots were rooted in different concentrations of indolbutiric acid (IBA) and acclimatized in greenhouse. The best temperature for short‑term storage in vitro and survival of the genotypes was 20°C. In the propagation phase, the highest number of shoots per explant was found in the subcultures 4 and 5, with averages of 4.9 and 4.8 shoots per explant, respectively. In the rooting phase, the best results for number of roots were obtained using a culture medium supplemented with 0.4 μmol L‑1 of IBA, with an average of three roots per shoot. During the acclimation phase, a survival rate higher than 95% was achieved after 30 days in the greenhouse. Grapevine genotypes maintained for six months in vitro, at 20oC, can be micropropagated in large scale.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCNPqSilva, Rafael de CarvalhoLuis, Zanderluce GomesScherwinski-Pereira, Jonny Everson2012-04-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/9869Pesquisa Agropecuaria Brasileira; v.47, n.3, mar. 2012; 344-350Pesquisa Agropecuária Brasileira; v.47, n.3, mar. 2012; 344-3501678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAengporhttps://seer.sct.embrapa.br/index.php/pab/article/view/9869/6820https://seer.sct.embrapa.br/index.php/pab/article/view/9869/7446https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/9869/5150info:eu-repo/semantics/openAccess2012-08-03T14:35:01Zoai:ojs.seer.sct.embrapa.br:article/9869Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2012-08-03T14:35:01Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Short‑term storage in vitro and large‑scale propagation of grapevine genotypes Short‑term storage in vitro and large‑scale propagation of grapevine genotypes |
title |
Short‑term storage in vitro and large‑scale propagation of grapevine genotypes |
spellingShingle |
Short‑term storage in vitro and large‑scale propagation of grapevine genotypes Silva, Rafael de Carvalho Vitis; germplasm; ex situ conservation; in vitro conservation; micropropagation; minimal growth Vitis; germoplasma; conservação ex situ; conservação in vitro; micropropagação; crescimento mínimo |
title_short |
Short‑term storage in vitro and large‑scale propagation of grapevine genotypes |
title_full |
Short‑term storage in vitro and large‑scale propagation of grapevine genotypes |
title_fullStr |
Short‑term storage in vitro and large‑scale propagation of grapevine genotypes |
title_full_unstemmed |
Short‑term storage in vitro and large‑scale propagation of grapevine genotypes |
title_sort |
Short‑term storage in vitro and large‑scale propagation of grapevine genotypes |
author |
Silva, Rafael de Carvalho |
author_facet |
Silva, Rafael de Carvalho Luis, Zanderluce Gomes Scherwinski-Pereira, Jonny Everson |
author_role |
author |
author2 |
Luis, Zanderluce Gomes Scherwinski-Pereira, Jonny Everson |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
CNPq |
dc.contributor.author.fl_str_mv |
Silva, Rafael de Carvalho Luis, Zanderluce Gomes Scherwinski-Pereira, Jonny Everson |
dc.subject.por.fl_str_mv |
Vitis; germplasm; ex situ conservation; in vitro conservation; micropropagation; minimal growth Vitis; germoplasma; conservação ex situ; conservação in vitro; micropropagação; crescimento mínimo |
topic |
Vitis; germplasm; ex situ conservation; in vitro conservation; micropropagation; minimal growth Vitis; germoplasma; conservação ex situ; conservação in vitro; micropropagação; crescimento mínimo |
description |
The objective of this work was to evaluate the large‑scale propagation of grapevine genotypes after short‑term storage in vitro. Microshoots from ten grapevine genotypes were used. The following storage temperatures were evaluated: 10, 20, and 25°C. After short‑term storage, the shoots were propagated in up to five successive subcultures, to assess the large‑scale propagation of the germplasm maintained under conditions of minimal growth. The propagated shoots were rooted in different concentrations of indolbutiric acid (IBA) and acclimatized in greenhouse. The best temperature for short‑term storage in vitro and survival of the genotypes was 20°C. In the propagation phase, the highest number of shoots per explant was found in the subcultures 4 and 5, with averages of 4.9 and 4.8 shoots per explant, respectively. In the rooting phase, thebest results for number of roots were obtained using a culture medium supplemented with 0.4 μmol L‑1 of IBA, with an average of three roots per shoot. During the acclimation phase, a survival rate higher than 95% was achieved after 30 days in the greenhouse. Grapevine genotypes maintained for six months in vitro, at 20oC, can be micropropagated in large scale. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-04-23 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/9869 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/9869 |
dc.language.iso.fl_str_mv |
eng por |
language |
eng por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/9869/6820 https://seer.sct.embrapa.br/index.php/pab/article/view/9869/7446 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/9869/5150 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.47, n.3, mar. 2012; 344-350 Pesquisa Agropecuária Brasileira; v.47, n.3, mar. 2012; 344-350 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416652281348096 |