Evaluation of banana commercial micropropagation

Detalhes bibliográficos
Autor(a) principal: Pedroso de Oliveira, Roberto
Data de Publicação: 1997
Outros Autores: de Oliveira e Silva, Sebastião
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/4654
Resumo: The efficiency of a commercial micropropagation system of the Nanicão and Grand Naine triploid banana cultivars was evaluated. The in vitro culture was established in MS medium, supplemented with 30 g/L of sucrose and 0.18% of PhytagelTM, pH 5.7; bud multiplication was carried out in the same medium supplemented with 4.0 mg/L of BAP (benzilaminopurine). Plantlets were rooted in MS medium containing half the concentration of salts and supplemented with 30 g/L of sucrose and lacking growth regulators. After rooting, the plantlets were acclimatized in a green-house. Losses by contamination during the period of the in vitro establishment were 18.0% and 40.6% for cultivars Nanicão and Grand Naine, respectively. Average multiplication rates in the subcultures were 2.1 for 'Nanicão' and 2.4 for 'Grand Naine'. Two percent of the plantlets were lost during acclimation process. The average production of plantlets were 189.2 for 'Nanicão' and 198.8 for Grand Naine.
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spelling Evaluation of banana commercial micropropagationAvaliação da micropropagação comercial em bananeiraMusa sp.; Nanicão; Grand Naine; multiplication rateMusa sp.; Nanicão; Grande Naine; taxa de multiplicaçãoThe efficiency of a commercial micropropagation system of the Nanicão and Grand Naine triploid banana cultivars was evaluated. The in vitro culture was established in MS medium, supplemented with 30 g/L of sucrose and 0.18% of PhytagelTM, pH 5.7; bud multiplication was carried out in the same medium supplemented with 4.0 mg/L of BAP (benzilaminopurine). Plantlets were rooted in MS medium containing half the concentration of salts and supplemented with 30 g/L of sucrose and lacking growth regulators. After rooting, the plantlets were acclimatized in a green-house. Losses by contamination during the period of the in vitro establishment were 18.0% and 40.6% for cultivars Nanicão and Grand Naine, respectively. Average multiplication rates in the subcultures were 2.1 for 'Nanicão' and 2.4 for 'Grand Naine'. Two percent of the plantlets were lost during acclimation process. The average production of plantlets were 189.2 for 'Nanicão' and 198.8 for Grand Naine.O presente trabalho teve por objetivo avaliar a eficiência de um sistema de micropropagação em escala comercial das cultivares de bananeira (Musa sp.) Nanicão e Grande Naine. A cultura foi estabelecida em meio nutritivo MS, suplementado com 30 g/L de sacarose, 0,18% de PhytagelTM e pH 5,7; a multiplicação foi realizada no mesmo meio de cultura suplementado com 4,0 mg/L de BAP (benzilaminopurina). As plantas foram enraizadas em meio MS com metade da concentração de sais, suplementado com 30 g/L de sacarose, e sem reguladores de crescimento. Após o enraizamento, as plântulas foram aclimatadas em casa de vegetação. As perdas por contaminação durante o estabelecimento da cultura in vitro foram de 18,0% e 40,6% para as cultivares Nanicão e Grande Naine, respectivamente. A taxa média de multiplicação nos seis subcultivos foi de 2,1 para 'Nanicão' e 2,4 para 'Grande Naine'. As perdas na aclimatação totalizaram 2,0%. Com relação à eficiência do sistema de micropropagação, foram obtidas, em média, 189,2 mudas por explante viável após a fase de estabelecimento in vitro, para a cultivar Nanicão, e 198,8 para Grande Naine.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraPedroso de Oliveira, Robertode Oliveira e Silva, Sebastião1997-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/4654Pesquisa Agropecuaria Brasileira; v.32, n.4, abr. 1997; 415-420Pesquisa Agropecuária Brasileira; v.32, n.4, abr. 1997; 415-4201678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/4654/7242info:eu-repo/semantics/openAccess2015-03-13T18:14:04Zoai:ojs.seer.sct.embrapa.br:article/4654Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2015-03-13T18:14:04Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Evaluation of banana commercial micropropagation
Avaliação da micropropagação comercial em bananeira
title Evaluation of banana commercial micropropagation
spellingShingle Evaluation of banana commercial micropropagation
Pedroso de Oliveira, Roberto
Musa sp.; Nanicão; Grand Naine; multiplication rate
Musa sp.; Nanicão; Grande Naine; taxa de multiplicação
title_short Evaluation of banana commercial micropropagation
title_full Evaluation of banana commercial micropropagation
title_fullStr Evaluation of banana commercial micropropagation
title_full_unstemmed Evaluation of banana commercial micropropagation
title_sort Evaluation of banana commercial micropropagation
author Pedroso de Oliveira, Roberto
author_facet Pedroso de Oliveira, Roberto
de Oliveira e Silva, Sebastião
author_role author
author2 de Oliveira e Silva, Sebastião
author2_role author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Pedroso de Oliveira, Roberto
de Oliveira e Silva, Sebastião
dc.subject.por.fl_str_mv Musa sp.; Nanicão; Grand Naine; multiplication rate
Musa sp.; Nanicão; Grande Naine; taxa de multiplicação
topic Musa sp.; Nanicão; Grand Naine; multiplication rate
Musa sp.; Nanicão; Grande Naine; taxa de multiplicação
description The efficiency of a commercial micropropagation system of the Nanicão and Grand Naine triploid banana cultivars was evaluated. The in vitro culture was established in MS medium, supplemented with 30 g/L of sucrose and 0.18% of PhytagelTM, pH 5.7; bud multiplication was carried out in the same medium supplemented with 4.0 mg/L of BAP (benzilaminopurine). Plantlets were rooted in MS medium containing half the concentration of salts and supplemented with 30 g/L of sucrose and lacking growth regulators. After rooting, the plantlets were acclimatized in a green-house. Losses by contamination during the period of the in vitro establishment were 18.0% and 40.6% for cultivars Nanicão and Grand Naine, respectively. Average multiplication rates in the subcultures were 2.1 for 'Nanicão' and 2.4 for 'Grand Naine'. Two percent of the plantlets were lost during acclimation process. The average production of plantlets were 189.2 for 'Nanicão' and 198.8 for Grand Naine.
publishDate 1997
dc.date.none.fl_str_mv 1997-04-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/4654
url https://seer.sct.embrapa.br/index.php/pab/article/view/4654
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/4654/7242
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.32, n.4, abr. 1997; 415-420
Pesquisa Agropecuária Brasileira; v.32, n.4, abr. 1997; 415-420
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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