Genetic variation among accessions of the genus Manihot by ISSR markers
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/10850 |
Resumo: | The objective of this work was to evaluate the genetic diversity within and among accessions of Manihot by using ISSR markers. Five species and two varieties of Manihot, besides two species of the genus Croton, used as the out-group, were analyzed by using 20 oligonucleotide (Olii) ISSR UBC primers. To analyze the similarity index between species and accessions, the Jaccard and simple matching coefficients were used. The 20 Olii tested were highly polymorphic in all species analyzed, and 89.7% of the loci were polymorphic. A higher genetic variability is observed among different species of Manihot, such as M. dichotoma var. undulata and M. caerulescens, than among individuals of the same species, such as M. dichotoma and M. dichotoma var. undulata. |
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Genetic variation among accessions of the genus Manihot by ISSR markersVariabilidade genética entre acessos do gênero Manihot por meio de marcadores moleculares ISSRgermplasm; genetic diversity; cassava; polymorphism.germoplasma; diversidade genética; mandioca; polimorfismoThe objective of this work was to evaluate the genetic diversity within and among accessions of Manihot by using ISSR markers. Five species and two varieties of Manihot, besides two species of the genus Croton, used as the out-group, were analyzed by using 20 oligonucleotide (Olii) ISSR UBC primers. To analyze the similarity index between species and accessions, the Jaccard and simple matching coefficients were used. The 20 Olii tested were highly polymorphic in all species analyzed, and 89.7% of the loci were polymorphic. A higher genetic variability is observed among different species of Manihot, such as M. dichotoma var. undulata and M. caerulescens, than among individuals of the same species, such as M. dichotoma and M. dichotoma var. undulata.O objetivo deste trabalho foi avaliar a diversidade genética intra e interespecífica de acessos de Manihot por meio de marcadores ISSR. Foram analisadas cinco espécies e duas variedades de Manihot, além de duas espécies do gênero Croton, utilizadas como grupo externo, por meio de 20 oligonucleotídeos iniciadores (Olii) ISSR UBC. Para análise do índice de similaridade entre as espécies e os acessos foram utilizados os coeficientes de Jaccard e de 'simple matching'. Os 20 Olii testados foram altamente polimórficos para todas as espécies analisadas, e 89,7% dos locus foram polimórficos. Há maior similaridade genética entre espécies diferentes de Manihot, como M. dichotoma var. undulata e M. caerulescens, do que entre indivíduos da mesma espécie, como M. dichotoma e M. dichotoma var. undulata. Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária Brasileiraembrapa, cnpqPessoa da silva, Kaliny VeigaAlves, Alfredo Augusto da CunhaMartins, Maria Isabel Gomesde Melo, Cláusio Antônio Ferreirade Carvalho, Reginaldo2011-12-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/10850Pesquisa Agropecuaria Brasileira; v.46, n.9, set. 2011; 1082-1088Pesquisa Agropecuária Brasileira; v.46, n.9, set. 2011; 1082-10881678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/10850/6569https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/10850/6249info:eu-repo/semantics/openAccess2014-06-11T17:07:09Zoai:ojs.seer.sct.embrapa.br:article/10850Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-06-11T17:07:09Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Genetic variation among accessions of the genus Manihot by ISSR markers Variabilidade genética entre acessos do gênero Manihot por meio de marcadores moleculares ISSR |
title |
Genetic variation among accessions of the genus Manihot by ISSR markers |
spellingShingle |
Genetic variation among accessions of the genus Manihot by ISSR markers Pessoa da silva, Kaliny Veiga germplasm; genetic diversity; cassava; polymorphism. germoplasma; diversidade genética; mandioca; polimorfismo |
title_short |
Genetic variation among accessions of the genus Manihot by ISSR markers |
title_full |
Genetic variation among accessions of the genus Manihot by ISSR markers |
title_fullStr |
Genetic variation among accessions of the genus Manihot by ISSR markers |
title_full_unstemmed |
Genetic variation among accessions of the genus Manihot by ISSR markers |
title_sort |
Genetic variation among accessions of the genus Manihot by ISSR markers |
author |
Pessoa da silva, Kaliny Veiga |
author_facet |
Pessoa da silva, Kaliny Veiga Alves, Alfredo Augusto da Cunha Martins, Maria Isabel Gomes de Melo, Cláusio Antônio Ferreira de Carvalho, Reginaldo |
author_role |
author |
author2 |
Alves, Alfredo Augusto da Cunha Martins, Maria Isabel Gomes de Melo, Cláusio Antônio Ferreira de Carvalho, Reginaldo |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
embrapa, cnpq |
dc.contributor.author.fl_str_mv |
Pessoa da silva, Kaliny Veiga Alves, Alfredo Augusto da Cunha Martins, Maria Isabel Gomes de Melo, Cláusio Antônio Ferreira de Carvalho, Reginaldo |
dc.subject.por.fl_str_mv |
germplasm; genetic diversity; cassava; polymorphism. germoplasma; diversidade genética; mandioca; polimorfismo |
topic |
germplasm; genetic diversity; cassava; polymorphism. germoplasma; diversidade genética; mandioca; polimorfismo |
description |
The objective of this work was to evaluate the genetic diversity within and among accessions of Manihot by using ISSR markers. Five species and two varieties of Manihot, besides two species of the genus Croton, used as the out-group, were analyzed by using 20 oligonucleotide (Olii) ISSR UBC primers. To analyze the similarity index between species and accessions, the Jaccard and simple matching coefficients were used. The 20 Olii tested were highly polymorphic in all species analyzed, and 89.7% of the loci were polymorphic. A higher genetic variability is observed among different species of Manihot, such as M. dichotoma var. undulata and M. caerulescens, than among individuals of the same species, such as M. dichotoma and M. dichotoma var. undulata. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-14 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/10850 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/10850 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/10850/6569 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/10850/6249 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.46, n.9, set. 2011; 1082-1088 Pesquisa Agropecuária Brasileira; v.46, n.9, set. 2011; 1082-1088 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416652848627712 |