Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan

Detalhes bibliográficos
Autor(a) principal: Zaefizadeh, Mohammad
Data de Publicação: 2011
Outros Autores: Jamaati-e-Somarin, Shahzad, Ojaghi, Javid, Seyedi, Seyed Mehdi, Zabihi-e-Mahmoodabad, Roghayyeh, Ochi, Mehran
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/8127
Resumo: The objective of this study was to identify gliadin band patterns and the extent of genetic diversity in durum wheat genotypes from Northwestern Iran and the Republic of Azerbaijan. Gliadins from 46 landraces and four cultivars were evaluated through acid PAGE analyses. Sixty-six polymorphic bands and 81 patterns were identified. Twenty-four different motility bands and 22 patterns were found in the ω gliadin region with 14 polymorph bands and 20 patterns for α and γ gliadins, and 14 bands and 19 different patterns for β gliadins. The combination of these patterns generated 38 and 39 combinations for Gli-1 and Gli-2 loci, respectively. The genetic diversity index (H) was higher for α gliadins (0.924), followed by ω and γ gliadins (0.899 and 0.878, respectively), and for β gliadin patterns (0.866). Extensive polymorphism (H = 0.875) was observed in four gliadin pattern regions, with higher genetic diversity in the Iranian landraces than in the Azerbaijani ones. Each genotype had special identifying patterns in the gliadin acid PAGE analysis, and cluster analysis based on Jaccard's similarity coefficients formed six groups. Gliadin has a simple, repeatable and economic analysis, and can be used in genetic studies.
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spelling Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and AzerbaijanDiversidade genética para padrões de gliadinas de raças locais de trigo duro no Noroeste do Iran e no AzerbaijãoTriticum durum; acid PAGE analysis; germplasm; traditional agriculture.Triticum durum; análise "acid PAGE"; germoplasma; agricultura tradicionalThe objective of this study was to identify gliadin band patterns and the extent of genetic diversity in durum wheat genotypes from Northwestern Iran and the Republic of Azerbaijan. Gliadins from 46 landraces and four cultivars were evaluated through acid PAGE analyses. Sixty-six polymorphic bands and 81 patterns were identified. Twenty-four different motility bands and 22 patterns were found in the ω gliadin region with 14 polymorph bands and 20 patterns for α and γ gliadins, and 14 bands and 19 different patterns for β gliadins. The combination of these patterns generated 38 and 39 combinations for Gli-1 and Gli-2 loci, respectively. The genetic diversity index (H) was higher for α gliadins (0.924), followed by ω and γ gliadins (0.899 and 0.878, respectively), and for β gliadin patterns (0.866). Extensive polymorphism (H = 0.875) was observed in four gliadin pattern regions, with higher genetic diversity in the Iranian landraces than in the Azerbaijani ones. Each genotype had special identifying patterns in the gliadin acid PAGE analysis, and cluster analysis based on Jaccard's similarity coefficients formed six groups. Gliadin has a simple, repeatable and economic analysis, and can be used in genetic studies. O objetivo deste trabalho foi identificar padrões de bandas de gliadinas e a extenção da variabilidade genética em genótipos de trigo duro do Noroeste do Iran e da República do Azerbaijão. Gliadinas de 46 raças locais e de quatro cultivares foram avaliadas por meio de análises "acid PAGE". Sessenta e seis bandas polimórficas e 81 padrões foram identificados. Foram encontradas 24 bandas de mobilidade e 22 padrões na região da gliadina ω, com 14 bandas polimorfas e 20 padrões para as gliadinas α e γ , e 14 bandas e 19 padrões diferentes para as gliadinas β. A combinação desses padrões gerou 38 e 39 combinações, para os locos Gli-1 e Gli-2, respectivamente. O índice de diversidade genética (H) foi maior para as gliadinas α (0,924), seguidas pelas gliadinas ω e γ (0,899 e 0,878, respectivamente), e pela gliadina β (0,866). Alto polimorfismo (H = 0,875) foi observado em quatro regiões dos padrões de gliadinas obtidos, com diversidade genética maior nas raças locais iranianas do que nas do Azerbaijão. Cada genótipo apresentou padrões de identificação especiais na análise "acid PAGE" de gliadinas, e a análise de agrupamento baseada no coeficiente de similaridade de Jaccard formou seis grupos. A gliadina é de análise simples, repetível e econômica, e pode ser usada em estudos genéticos.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraZaefizadeh, MohammadJamaati-e-Somarin, ShahzadOjaghi, JavidSeyedi, Seyed MehdiZabihi-e-Mahmoodabad, RoghayyehOchi, Mehran2011-03-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/8127Pesquisa Agropecuaria Brasileira; v.45, n.12, dez. 2010; 1425-1432Pesquisa Agropecuária Brasileira; v.45, n.12, dez. 2010; 1425-14321678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/8127/6163info:eu-repo/semantics/openAccess2014-11-14T18:43:01Zoai:ojs.seer.sct.embrapa.br:article/8127Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-11-14T18:43:01Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan
Diversidade genética para padrões de gliadinas de raças locais de trigo duro no Noroeste do Iran e no Azerbaijão
title Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan
spellingShingle Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan
Zaefizadeh, Mohammad
Triticum durum; acid PAGE analysis; germplasm; traditional agriculture.
Triticum durum; análise "acid PAGE"; germoplasma; agricultura tradicional
title_short Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan
title_full Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan
title_fullStr Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan
title_full_unstemmed Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan
title_sort Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan
author Zaefizadeh, Mohammad
author_facet Zaefizadeh, Mohammad
Jamaati-e-Somarin, Shahzad
Ojaghi, Javid
Seyedi, Seyed Mehdi
Zabihi-e-Mahmoodabad, Roghayyeh
Ochi, Mehran
author_role author
author2 Jamaati-e-Somarin, Shahzad
Ojaghi, Javid
Seyedi, Seyed Mehdi
Zabihi-e-Mahmoodabad, Roghayyeh
Ochi, Mehran
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Zaefizadeh, Mohammad
Jamaati-e-Somarin, Shahzad
Ojaghi, Javid
Seyedi, Seyed Mehdi
Zabihi-e-Mahmoodabad, Roghayyeh
Ochi, Mehran
dc.subject.por.fl_str_mv Triticum durum; acid PAGE analysis; germplasm; traditional agriculture.
Triticum durum; análise "acid PAGE"; germoplasma; agricultura tradicional
topic Triticum durum; acid PAGE analysis; germplasm; traditional agriculture.
Triticum durum; análise "acid PAGE"; germoplasma; agricultura tradicional
description The objective of this study was to identify gliadin band patterns and the extent of genetic diversity in durum wheat genotypes from Northwestern Iran and the Republic of Azerbaijan. Gliadins from 46 landraces and four cultivars were evaluated through acid PAGE analyses. Sixty-six polymorphic bands and 81 patterns were identified. Twenty-four different motility bands and 22 patterns were found in the ω gliadin region with 14 polymorph bands and 20 patterns for α and γ gliadins, and 14 bands and 19 different patterns for β gliadins. The combination of these patterns generated 38 and 39 combinations for Gli-1 and Gli-2 loci, respectively. The genetic diversity index (H) was higher for α gliadins (0.924), followed by ω and γ gliadins (0.899 and 0.878, respectively), and for β gliadin patterns (0.866). Extensive polymorphism (H = 0.875) was observed in four gliadin pattern regions, with higher genetic diversity in the Iranian landraces than in the Azerbaijani ones. Each genotype had special identifying patterns in the gliadin acid PAGE analysis, and cluster analysis based on Jaccard's similarity coefficients formed six groups. Gliadin has a simple, repeatable and economic analysis, and can be used in genetic studies.
publishDate 2011
dc.date.none.fl_str_mv 2011-03-22
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/8127
url https://seer.sct.embrapa.br/index.php/pab/article/view/8127
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/8127/6163
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.45, n.12, dez. 2010; 1425-1432
Pesquisa Agropecuária Brasileira; v.45, n.12, dez. 2010; 1425-1432
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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