Ethylene and hydrogen peroxide in the aerenchyma formation in maize tolerant to intermittent flooding
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/21427 |
Resumo: | The objective of this work was to evaluate the role of ethylene and hydrogen peroxide (H2O2) in the aerenchyma formation in genetic selection cycles of the maize cultivar BRS 4154, under flooding. Plants from cycles C1 and C18 were flooded for 7 days, with roots collected at 0 (control, no flooding), 1, and 7 days. The following were evaluated: gene expression of the enzymes ACC synthase (ACS), ACC oxidase (ACO), superoxide dismutase (SOD), and ascorbate peroxidase (APX); ethylene production and H2O2 content; enzymatic activity of ACO; and aerenchyma proportion in the cortex. There was no expression of ACS and ACO. ACO activity and ethylene production varied. SOD expression was higher in C1 plants and that of APX in C18 plants, with reduction at 7 days. H2O2 content did not differ between treatments. The proportion of aerenchyma increased with time, being higher in C18 plants and related to the aerenchyma formation rate. The flooding time and the tolerance level of the selection cycle influence ethylene production. The expression of APX indicates greater H2O2 production at the beginning of flooding. |
id |
EMBRAPA-4_ec38da5a8411e0d4370ebf28bc563f03 |
---|---|
oai_identifier_str |
oai:ojs.seer.sct.embrapa.br:article/21427 |
network_acronym_str |
EMBRAPA-4 |
network_name_str |
Pesquisa Agropecuária Brasileira (Online) |
repository_id_str |
|
spelling |
Ethylene and hydrogen peroxide in the aerenchyma formation in maize tolerant to intermittent floodingEtileno e peróxido de hidrogênio na formação de aerênquima em milho tolerante a alagamento intermitenteZea mays; root anatomy; enzyme activity; gene expressionZea mays; anatomia radicular; atividade enzimática; expressão gênicaThe objective of this work was to evaluate the role of ethylene and hydrogen peroxide (H2O2) in the aerenchyma formation in genetic selection cycles of the maize cultivar BRS 4154, under flooding. Plants from cycles C1 and C18 were flooded for 7 days, with roots collected at 0 (control, no flooding), 1, and 7 days. The following were evaluated: gene expression of the enzymes ACC synthase (ACS), ACC oxidase (ACO), superoxide dismutase (SOD), and ascorbate peroxidase (APX); ethylene production and H2O2 content; enzymatic activity of ACO; and aerenchyma proportion in the cortex. There was no expression of ACS and ACO. ACO activity and ethylene production varied. SOD expression was higher in C1 plants and that of APX in C18 plants, with reduction at 7 days. H2O2 content did not differ between treatments. The proportion of aerenchyma increased with time, being higher in C18 plants and related to the aerenchyma formation rate. The flooding time and the tolerance level of the selection cycle influence ethylene production. The expression of APX indicates greater H2O2 production at the beginning of flooding.O objetivo deste trabalho foi avaliar o papel do etileno e do peróxido de hidrogênio (H2O2) na formação do aerênquima em ciclos de seleção genética da cultivar de milho BRS 4154, sob alagamento. Plantas dos ciclos C1 e C18 foram submetidas a alagamento por 7 dias, com coleta das raízes aos 0 (controle, sem alagamento), 1 e 7 dias. Foram analisados: a expressão gênica das enzimas ACC sintase (ACS), ACC oxidase (ACO), dismutase do superóxido (SOD) e peroxidase do ascorbato (APX); a produção de etileno e o conteúdo de H2O2; a atividade da enzima ACO; e a proporção de aerênquima no córtex. Não houve expressão de ACS e ACO. Houve variação na atividade de ACO e na produção de etileno. A expressão da SOD foi maior em plantas C1 e a da APX, em C18, com redução aos 7 dias. O conteúdo de H2O2 não diferiu entre os tratamentos. A proporção de aerênquima aumentou com o tempo, tendo sido maior em plantas C18 e relacionada à taxa de formação do aerênquima. O tempo de alagamento e o nível de tolerância do ciclo de seleção influenciam a produção do etileno. A expressão da APX indica maior produção de H2O2 no início do alagamento.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraFundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Pires, Marinês FerreiraCastro, Evaristo Mauro deMagalhães, Paulo CesarSilva Neta, Izabel CostaMonteiro, Aline Gomes Dias Pinto2015-10-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/21427Pesquisa Agropecuaria Brasileira; v.50, n.9, set. 2015; 779-787Pesquisa Agropecuária Brasileira; v.50, n.9, set. 2015; 779-7871678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/21427/13046https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/21427/13540https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/21427/13541Direitos autorais 2015 Pesquisa Agropecuária Brasileirainfo:eu-repo/semantics/openAccess2015-10-09T16:54:26Zoai:ojs.seer.sct.embrapa.br:article/21427Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2015-10-09T16:54:26Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Ethylene and hydrogen peroxide in the aerenchyma formation in maize tolerant to intermittent flooding Etileno e peróxido de hidrogênio na formação de aerênquima em milho tolerante a alagamento intermitente |
title |
Ethylene and hydrogen peroxide in the aerenchyma formation in maize tolerant to intermittent flooding |
spellingShingle |
Ethylene and hydrogen peroxide in the aerenchyma formation in maize tolerant to intermittent flooding Pires, Marinês Ferreira Zea mays; root anatomy; enzyme activity; gene expression Zea mays; anatomia radicular; atividade enzimática; expressão gênica |
title_short |
Ethylene and hydrogen peroxide in the aerenchyma formation in maize tolerant to intermittent flooding |
title_full |
Ethylene and hydrogen peroxide in the aerenchyma formation in maize tolerant to intermittent flooding |
title_fullStr |
Ethylene and hydrogen peroxide in the aerenchyma formation in maize tolerant to intermittent flooding |
title_full_unstemmed |
Ethylene and hydrogen peroxide in the aerenchyma formation in maize tolerant to intermittent flooding |
title_sort |
Ethylene and hydrogen peroxide in the aerenchyma formation in maize tolerant to intermittent flooding |
author |
Pires, Marinês Ferreira |
author_facet |
Pires, Marinês Ferreira Castro, Evaristo Mauro de Magalhães, Paulo Cesar Silva Neta, Izabel Costa Monteiro, Aline Gomes Dias Pinto |
author_role |
author |
author2 |
Castro, Evaristo Mauro de Magalhães, Paulo Cesar Silva Neta, Izabel Costa Monteiro, Aline Gomes Dias Pinto |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Fundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) |
dc.contributor.author.fl_str_mv |
Pires, Marinês Ferreira Castro, Evaristo Mauro de Magalhães, Paulo Cesar Silva Neta, Izabel Costa Monteiro, Aline Gomes Dias Pinto |
dc.subject.por.fl_str_mv |
Zea mays; root anatomy; enzyme activity; gene expression Zea mays; anatomia radicular; atividade enzimática; expressão gênica |
topic |
Zea mays; root anatomy; enzyme activity; gene expression Zea mays; anatomia radicular; atividade enzimática; expressão gênica |
description |
The objective of this work was to evaluate the role of ethylene and hydrogen peroxide (H2O2) in the aerenchyma formation in genetic selection cycles of the maize cultivar BRS 4154, under flooding. Plants from cycles C1 and C18 were flooded for 7 days, with roots collected at 0 (control, no flooding), 1, and 7 days. The following were evaluated: gene expression of the enzymes ACC synthase (ACS), ACC oxidase (ACO), superoxide dismutase (SOD), and ascorbate peroxidase (APX); ethylene production and H2O2 content; enzymatic activity of ACO; and aerenchyma proportion in the cortex. There was no expression of ACS and ACO. ACO activity and ethylene production varied. SOD expression was higher in C1 plants and that of APX in C18 plants, with reduction at 7 days. H2O2 content did not differ between treatments. The proportion of aerenchyma increased with time, being higher in C18 plants and related to the aerenchyma formation rate. The flooding time and the tolerance level of the selection cycle influence ethylene production. The expression of APX indicates greater H2O2 production at the beginning of flooding. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-09 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/21427 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/21427 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/21427/13046 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/21427/13540 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/21427/13541 |
dc.rights.driver.fl_str_mv |
Direitos autorais 2015 Pesquisa Agropecuária Brasileira info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Direitos autorais 2015 Pesquisa Agropecuária Brasileira |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.50, n.9, set. 2015; 779-787 Pesquisa Agropecuária Brasileira; v.50, n.9, set. 2015; 779-787 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
_version_ |
1793416702364483584 |