Ethylene and hydrogen peroxide in the aerenchyma formation in maize tolerant to intermittent flooding

Detalhes bibliográficos
Autor(a) principal: Pires, Marinês Ferreira
Data de Publicação: 2015
Outros Autores: Castro, Evaristo Mauro de, Magalhães, Paulo Cesar, Silva Neta, Izabel Costa, Monteiro, Aline Gomes Dias Pinto
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/21427
Resumo: The objective of this work was to evaluate the role of ethylene and hydrogen peroxide (H2O2) in the aerenchyma formation in genetic selection cycles of the maize cultivar BRS 4154, under flooding. Plants from cycles C1 and C18 were flooded for 7 days, with roots collected at 0 (control, no flooding), 1, and 7 days. The following were evaluated: gene expression of the enzymes ACC synthase (ACS), ACC oxidase (ACO), superoxide dismutase (SOD), and ascorbate peroxidase (APX); ethylene production and H2O2 content; enzymatic activity of ACO; and aerenchyma proportion in the cortex. There was no expression of ACS and ACO. ACO activity and ethylene production varied. SOD expression was higher in C1 plants and that of APX in C18 plants, with reduction at 7 days. H2O2 content did not differ between treatments. The proportion of aerenchyma increased with time, being higher in C18 plants and related to the aerenchyma formation rate. The flooding time and the tolerance level of the selection cycle influence ethylene production. The expression of APX indicates greater H2O2 production at the beginning of flooding.
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spelling Ethylene and hydrogen peroxide in the aerenchyma formation in maize tolerant to intermittent floodingEtileno e peróxido de hidrogênio na formação de aerênquima em milho tolerante a alagamento intermitenteZea mays; root anatomy; enzyme activity; gene expressionZea mays; anatomia radicular; atividade enzimática; expressão gênicaThe objective of this work was to evaluate the role of ethylene and hydrogen peroxide (H2O2) in the aerenchyma formation in genetic selection cycles of the maize cultivar BRS 4154, under flooding. Plants from cycles C1 and C18 were flooded for 7 days, with roots collected at 0 (control, no flooding), 1, and 7 days. The following were evaluated: gene expression of the enzymes ACC synthase (ACS), ACC oxidase (ACO), superoxide dismutase (SOD), and ascorbate peroxidase (APX); ethylene production and H2O2 content; enzymatic activity of ACO; and aerenchyma proportion in the cortex. There was no expression of ACS and ACO. ACO activity and ethylene production varied. SOD expression was higher in C1 plants and that of APX in C18 plants, with reduction at 7 days. H2O2 content did not differ between treatments. The proportion of aerenchyma increased with time, being higher in C18 plants and related to the aerenchyma formation rate. The flooding time and the tolerance level of the selection cycle influence ethylene production. The expression of APX indicates greater H2O2 production at the beginning of flooding.O objetivo deste trabalho foi avaliar o papel do etileno e do peróxido de hidrogênio (H2O2) na formação do aerênquima em ciclos de seleção genética da cultivar de milho BRS 4154, sob alagamento. Plantas dos ciclos C1 e C18 foram submetidas a alagamento por 7 dias, com coleta das raízes aos 0 (controle, sem alagamento), 1 e 7 dias. Foram analisados: a expressão gênica das enzimas ACC sintase (ACS), ACC oxidase (ACO), dismutase do superóxido (SOD) e peroxidase do ascorbato (APX); a produção de etileno e o conteúdo de H2O2; a atividade da enzima ACO; e a proporção de aerênquima no córtex. Não houve expressão de ACS e ACO. Houve variação na atividade de ACO e na produção de etileno. A expressão da SOD foi maior em plantas C1 e a da APX, em C18, com redução aos 7 dias. O conteúdo de H2O2 não diferiu entre os tratamentos. A proporção de aerênquima aumentou com o tempo, tendo sido maior em plantas C18 e relacionada à taxa de formação do aerênquima. O tempo de alagamento e o nível de tolerância do ciclo de seleção influenciam a produção do etileno. A expressão da APX indica maior produção de H2O2 no início do alagamento.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraFundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Pires, Marinês FerreiraCastro, Evaristo Mauro deMagalhães, Paulo CesarSilva Neta, Izabel CostaMonteiro, Aline Gomes Dias Pinto2015-10-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/21427Pesquisa Agropecuaria Brasileira; v.50, n.9, set. 2015; 779-787Pesquisa Agropecuária Brasileira; v.50, n.9, set. 2015; 779-7871678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/21427/13046https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/21427/13540https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/21427/13541Direitos autorais 2015 Pesquisa Agropecuária Brasileirainfo:eu-repo/semantics/openAccess2015-10-09T16:54:26Zoai:ojs.seer.sct.embrapa.br:article/21427Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2015-10-09T16:54:26Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Ethylene and hydrogen peroxide in the aerenchyma formation in maize tolerant to intermittent flooding
Etileno e peróxido de hidrogênio na formação de aerênquima em milho tolerante a alagamento intermitente
title Ethylene and hydrogen peroxide in the aerenchyma formation in maize tolerant to intermittent flooding
spellingShingle Ethylene and hydrogen peroxide in the aerenchyma formation in maize tolerant to intermittent flooding
Pires, Marinês Ferreira
Zea mays; root anatomy; enzyme activity; gene expression
Zea mays; anatomia radicular; atividade enzimática; expressão gênica
title_short Ethylene and hydrogen peroxide in the aerenchyma formation in maize tolerant to intermittent flooding
title_full Ethylene and hydrogen peroxide in the aerenchyma formation in maize tolerant to intermittent flooding
title_fullStr Ethylene and hydrogen peroxide in the aerenchyma formation in maize tolerant to intermittent flooding
title_full_unstemmed Ethylene and hydrogen peroxide in the aerenchyma formation in maize tolerant to intermittent flooding
title_sort Ethylene and hydrogen peroxide in the aerenchyma formation in maize tolerant to intermittent flooding
author Pires, Marinês Ferreira
author_facet Pires, Marinês Ferreira
Castro, Evaristo Mauro de
Magalhães, Paulo Cesar
Silva Neta, Izabel Costa
Monteiro, Aline Gomes Dias Pinto
author_role author
author2 Castro, Evaristo Mauro de
Magalhães, Paulo Cesar
Silva Neta, Izabel Costa
Monteiro, Aline Gomes Dias Pinto
author2_role author
author
author
author
dc.contributor.none.fl_str_mv
Fundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.contributor.author.fl_str_mv Pires, Marinês Ferreira
Castro, Evaristo Mauro de
Magalhães, Paulo Cesar
Silva Neta, Izabel Costa
Monteiro, Aline Gomes Dias Pinto
dc.subject.por.fl_str_mv Zea mays; root anatomy; enzyme activity; gene expression
Zea mays; anatomia radicular; atividade enzimática; expressão gênica
topic Zea mays; root anatomy; enzyme activity; gene expression
Zea mays; anatomia radicular; atividade enzimática; expressão gênica
description The objective of this work was to evaluate the role of ethylene and hydrogen peroxide (H2O2) in the aerenchyma formation in genetic selection cycles of the maize cultivar BRS 4154, under flooding. Plants from cycles C1 and C18 were flooded for 7 days, with roots collected at 0 (control, no flooding), 1, and 7 days. The following were evaluated: gene expression of the enzymes ACC synthase (ACS), ACC oxidase (ACO), superoxide dismutase (SOD), and ascorbate peroxidase (APX); ethylene production and H2O2 content; enzymatic activity of ACO; and aerenchyma proportion in the cortex. There was no expression of ACS and ACO. ACO activity and ethylene production varied. SOD expression was higher in C1 plants and that of APX in C18 plants, with reduction at 7 days. H2O2 content did not differ between treatments. The proportion of aerenchyma increased with time, being higher in C18 plants and related to the aerenchyma formation rate. The flooding time and the tolerance level of the selection cycle influence ethylene production. The expression of APX indicates greater H2O2 production at the beginning of flooding.
publishDate 2015
dc.date.none.fl_str_mv 2015-10-09
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/21427
url https://seer.sct.embrapa.br/index.php/pab/article/view/21427
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/21427/13046
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/21427/13540
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/21427/13541
dc.rights.driver.fl_str_mv Direitos autorais 2015 Pesquisa Agropecuária Brasileira
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Direitos autorais 2015 Pesquisa Agropecuária Brasileira
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.50, n.9, set. 2015; 779-787
Pesquisa Agropecuária Brasileira; v.50, n.9, set. 2015; 779-787
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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