Development of ic-Elisa for the screening of aflatoxin contamination in the peanut production chain
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
| Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/25215 |
Resumo: | The objective of this work was to standardize and validate an indirect competitive enzyme-linked immunosorbent assay (ic-Elisa), as a low-cost tool, to monitor the presence of aflatoxin in common and blanched peanuts (Arachis hypogaea) in the production chain. The presence of aflatoxin B1, moisture content, and water activity were analyzed in 60 samples of the peanut cultivar Runner IAC 886, from the 2014/2015 and 2015/2016 harvests of the region of Alta Paulista, in the state of São Paulo, Brazil. The validation showed an adequate linearity (R2 = 0.999), and limits of detection and quantification of 1.13 and 3.59 μg kg-1, respectively. Recovery rates of 104, 102, and 107% at the concentrations of 4, 10, and 20 μg kg-1 aflatoxin B1, respectively, were also recorded. The ic-Elisa showed a good reproducibility with a high-intraday precision, with 1.87% coefficient of variation (CV), and interday precision with 6.75% CV. The moisture content ranged from 4.0 to 7.2% (mean of 5.8%), and the water activity from 0.4848 to 0.6997 (mean of 0.5990) for the tested samples. Aflatoxin B1 was present in concentrations ranging from 1.13 to 29.2 μg kg-1, with only two samples (3.3%) exceeding the maximum allowed limit of 20 μg kg-1. The ic-ELISA developed here is an accessible tool for the rapid monitoring of aflatoxin contamination in the peanut production chain |
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Development of ic-Elisa for the screening of aflatoxin contamination in the peanut production chainDesenvolvimento de ic-Elisa para o rastreio da contaminação por aflatoxinas na cadeia de produção de amendoimAspergillus; aflatoxin B1; blanched peanuts; contamination; mycotoxinAspergillus; aflatoxina B1; amendoim “branqueado”; contaminação; micotoxinaThe objective of this work was to standardize and validate an indirect competitive enzyme-linked immunosorbent assay (ic-Elisa), as a low-cost tool, to monitor the presence of aflatoxin in common and blanched peanuts (Arachis hypogaea) in the production chain. The presence of aflatoxin B1, moisture content, and water activity were analyzed in 60 samples of the peanut cultivar Runner IAC 886, from the 2014/2015 and 2015/2016 harvests of the region of Alta Paulista, in the state of São Paulo, Brazil. The validation showed an adequate linearity (R2 = 0.999), and limits of detection and quantification of 1.13 and 3.59 μg kg-1, respectively. Recovery rates of 104, 102, and 107% at the concentrations of 4, 10, and 20 μg kg-1 aflatoxin B1, respectively, were also recorded. The ic-Elisa showed a good reproducibility with a high-intraday precision, with 1.87% coefficient of variation (CV), and interday precision with 6.75% CV. The moisture content ranged from 4.0 to 7.2% (mean of 5.8%), and the water activity from 0.4848 to 0.6997 (mean of 0.5990) for the tested samples. Aflatoxin B1 was present in concentrations ranging from 1.13 to 29.2 μg kg-1, with only two samples (3.3%) exceeding the maximum allowed limit of 20 μg kg-1. The ic-ELISA developed here is an accessible tool for the rapid monitoring of aflatoxin contamination in the peanut production chainO objetivo deste trabalho foi padronizar e validar um imunoensaio enzimático indireto competitivo (ic-Elisa), como ferramenta de baixo custo, para monitorar a presença de aflatoxina em amendoim comum (Arachis hypogaea) e “branqueado”, na cadeia de produção. A presença de aflatoxina B1, o teor de umidade e a atividade de água foram analisados em 60 amostras da cultivar de amendoim Runner IAC 886, das safras 2014/2015 e 2015/2016 da região da Alta Paulista, no Estado de São Paulo. A validação apresentou linearidade adequada (R2 = 0,999) e limite de detecção e quantificação de 1,13 e 3,59 µg kg-1, respectivamente. Taxas de recuperação de 104, 102 e 107% para as concentrações de 4, 10 e 20 µg kg-1 de aflatoxina B1, respectivamente, também foram registradas. O ic-Elisa mostrou boa repetibilidade, com alta precisão intradia com 1,87% de coeficiente de variação (CV), e de precisão interdia com 6,75% de CV. O teor de umidade variou de 4,0 a 7,2% (média de 5,8 %), e a atividade de água, de 0,4848 a 0,6997 (média de 0,5990), para as amostras testadas. A aflatoxina B1 esteve presente em concentrações que variaram entre 1,13 e 29,2 µg kg-1, com apenas duas amostras (3,3%) que excederam o limite máximo permitido de 20 µg kg-1. O ic-Elisa desenvolvido aqui é uma ferramenta acessível para o monitoramento rápido da contaminação por aflatoxinas na cadeia produtiva de amendoim.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes)Fundação AraucáriaJapan International Cooperation Agency (Jica)Silva, André Ribeiro daZanin, Lívia Montanheiro MédiceIshikawa, Angélica TiemeYamashita, Cassia Reika TakabayashiFracalossi, Felipe PedoteAmorim, Thaís MarquesItano, Eiko NakagawaKawamaura, OsamuHirooka, Elisa Yoko2018-05-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/25215Pesquisa Agropecuaria Brasileira; v.53, n.3, mar. 2018; 361-370Pesquisa Agropecuária Brasileira; v.53, n.3, mar. 2018; 361-3701678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/25215/14152https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25215/17000Direitos autorais 2018 Pesquisa Agropecuária Brasileirainfo:eu-repo/semantics/openAccess2018-05-08T11:31:42Zoai:ojs.seer.sct.embrapa.br:article/25215Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2018-05-08T11:31:42Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
| dc.title.none.fl_str_mv |
Development of ic-Elisa for the screening of aflatoxin contamination in the peanut production chain Desenvolvimento de ic-Elisa para o rastreio da contaminação por aflatoxinas na cadeia de produção de amendoim |
| title |
Development of ic-Elisa for the screening of aflatoxin contamination in the peanut production chain |
| spellingShingle |
Development of ic-Elisa for the screening of aflatoxin contamination in the peanut production chain Silva, André Ribeiro da Aspergillus; aflatoxin B1; blanched peanuts; contamination; mycotoxin Aspergillus; aflatoxina B1; amendoim “branqueado”; contaminação; micotoxina |
| title_short |
Development of ic-Elisa for the screening of aflatoxin contamination in the peanut production chain |
| title_full |
Development of ic-Elisa for the screening of aflatoxin contamination in the peanut production chain |
| title_fullStr |
Development of ic-Elisa for the screening of aflatoxin contamination in the peanut production chain |
| title_full_unstemmed |
Development of ic-Elisa for the screening of aflatoxin contamination in the peanut production chain |
| title_sort |
Development of ic-Elisa for the screening of aflatoxin contamination in the peanut production chain |
| author |
Silva, André Ribeiro da |
| author_facet |
Silva, André Ribeiro da Zanin, Lívia Montanheiro Médice Ishikawa, Angélica Tieme Yamashita, Cassia Reika Takabayashi Fracalossi, Felipe Pedote Amorim, Thaís Marques Itano, Eiko Nakagawa Kawamaura, Osamu Hirooka, Elisa Yoko |
| author_role |
author |
| author2 |
Zanin, Lívia Montanheiro Médice Ishikawa, Angélica Tieme Yamashita, Cassia Reika Takabayashi Fracalossi, Felipe Pedote Amorim, Thaís Marques Itano, Eiko Nakagawa Kawamaura, Osamu Hirooka, Elisa Yoko |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes) Fundação Araucária Japan International Cooperation Agency (Jica) |
| dc.contributor.author.fl_str_mv |
Silva, André Ribeiro da Zanin, Lívia Montanheiro Médice Ishikawa, Angélica Tieme Yamashita, Cassia Reika Takabayashi Fracalossi, Felipe Pedote Amorim, Thaís Marques Itano, Eiko Nakagawa Kawamaura, Osamu Hirooka, Elisa Yoko |
| dc.subject.por.fl_str_mv |
Aspergillus; aflatoxin B1; blanched peanuts; contamination; mycotoxin Aspergillus; aflatoxina B1; amendoim “branqueado”; contaminação; micotoxina |
| topic |
Aspergillus; aflatoxin B1; blanched peanuts; contamination; mycotoxin Aspergillus; aflatoxina B1; amendoim “branqueado”; contaminação; micotoxina |
| description |
The objective of this work was to standardize and validate an indirect competitive enzyme-linked immunosorbent assay (ic-Elisa), as a low-cost tool, to monitor the presence of aflatoxin in common and blanched peanuts (Arachis hypogaea) in the production chain. The presence of aflatoxin B1, moisture content, and water activity were analyzed in 60 samples of the peanut cultivar Runner IAC 886, from the 2014/2015 and 2015/2016 harvests of the region of Alta Paulista, in the state of São Paulo, Brazil. The validation showed an adequate linearity (R2 = 0.999), and limits of detection and quantification of 1.13 and 3.59 μg kg-1, respectively. Recovery rates of 104, 102, and 107% at the concentrations of 4, 10, and 20 μg kg-1 aflatoxin B1, respectively, were also recorded. The ic-Elisa showed a good reproducibility with a high-intraday precision, with 1.87% coefficient of variation (CV), and interday precision with 6.75% CV. The moisture content ranged from 4.0 to 7.2% (mean of 5.8%), and the water activity from 0.4848 to 0.6997 (mean of 0.5990) for the tested samples. Aflatoxin B1 was present in concentrations ranging from 1.13 to 29.2 μg kg-1, with only two samples (3.3%) exceeding the maximum allowed limit of 20 μg kg-1. The ic-ELISA developed here is an accessible tool for the rapid monitoring of aflatoxin contamination in the peanut production chain |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-05-08 |
| dc.type.none.fl_str_mv |
|
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://seer.sct.embrapa.br/index.php/pab/article/view/25215 |
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https://seer.sct.embrapa.br/index.php/pab/article/view/25215 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/25215/14152 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25215/17000 |
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Direitos autorais 2018 Pesquisa Agropecuária Brasileira info:eu-repo/semantics/openAccess |
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Direitos autorais 2018 Pesquisa Agropecuária Brasileira |
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openAccess |
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application/pdf |
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Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
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Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
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Pesquisa Agropecuaria Brasileira; v.53, n.3, mar. 2018; 361-370 Pesquisa Agropecuária Brasileira; v.53, n.3, mar. 2018; 361-370 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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