In vitro conservation of blackberry genotypes under minimal growth conditions and subsequent large-scale micropropagation

Detalhes bibliográficos
Autor(a) principal: Gomes, Hugo Teixeira
Data de Publicação: 2017
Outros Autores: Bartos, Patrícia Monah Cunha, Andrade, Maíra Teixeira de, Almeida, Raphael Ferreira, Lacerda, Luciana Florencio de, Scherwinski-Pereira, Jonny Everson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/24722
Resumo: The objective of this work was to evaluate the micropropagation of blackberry (Rubus spp.) cultivars, after in vitro conservation under minimal growth conditions. Nodal segments of the 'Guarani', 'Caingangue', 'Ébano', and 'Xavante' genotypes were conserved under minimal growth conditions at 20ºC, for 15 months. Microshoots were regenerated and multiplied by up to five successive subcultures, when they were rooted and acclimatized. After 30 days of acclimatization in a greenhouse, rooted plantlets showed no significant losses. Blackberry cultivars can be conserved in vitro for 15 months, without subcultures and, after this time, they can be micropropagated on a large-scale, maintaining the regenerative potential and multiplication.
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spelling In vitro conservation of blackberry genotypes under minimal growth conditions and subsequent large-scale micropropagationConservação in vitro de cultivares de amoreira-preta em condições de crescimento mínimo e subsequente micropropagação em larga escalaRubus; ex situ conservation; plant genetic resources; plant propagation; slow-growthRubus; conservação ex situ; recursos genéticos de plantas; propagação de plantas; crescimento lentoThe objective of this work was to evaluate the micropropagation of blackberry (Rubus spp.) cultivars, after in vitro conservation under minimal growth conditions. Nodal segments of the 'Guarani', 'Caingangue', 'Ébano', and 'Xavante' genotypes were conserved under minimal growth conditions at 20ºC, for 15 months. Microshoots were regenerated and multiplied by up to five successive subcultures, when they were rooted and acclimatized. After 30 days of acclimatization in a greenhouse, rooted plantlets showed no significant losses. Blackberry cultivars can be conserved in vitro for 15 months, without subcultures and, after this time, they can be micropropagated on a large-scale, maintaining the regenerative potential and multiplication.O objetivo deste trabalho foi avaliar a micropropagação de cultivares de amoreira-preta (Rubus spp.), após a conservação in vitro em condições de crescimento mínimo. Segmentos nodais dos genótipos 'Guarani', 'Caingangue', 'Ébano' and 'Xavante' foram conservados sob crescimento mínimo à temperatura de 20ºC, por 15 meses. Os microbrotos foram regenerados e multiplicados por até cinco subcultivos sucessivos, quando então foram enraizados e aclimatizados. Após 30 dias de aclimatização em casa de vegetação, as plantas enraizadas não apresentaram perdas significativas. As cultivares de amoreira-preta podem ser conservadas in vitro por 15 meses, sem subcultivos e, após esse período, podem ser micropropagadas em larga escala, mantendo-se o potencial regenerativo e a multiplicação.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraGomes, Hugo TeixeiraBartos, Patrícia Monah CunhaAndrade, Maíra Teixeira deAlmeida, Raphael FerreiraLacerda, Luciana Florencio deScherwinski-Pereira, Jonny Everson2017-12-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/24722Pesquisa Agropecuaria Brasileira; v.52, n.12, dez. 2017; 1286-1290Pesquisa Agropecuária Brasileira; v.52, n.12, dez. 2017; 1286-12901678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/24722/14052https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/24722/16555Direitos autorais 2017 Pesquisa Agropecuária Brasileirainfo:eu-repo/semantics/openAccess2017-12-21T10:21:52Zoai:ojs.seer.sct.embrapa.br:article/24722Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2017-12-21T10:21:52Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv In vitro conservation of blackberry genotypes under minimal growth conditions and subsequent large-scale micropropagation
Conservação in vitro de cultivares de amoreira-preta em condições de crescimento mínimo e subsequente micropropagação em larga escala
title In vitro conservation of blackberry genotypes under minimal growth conditions and subsequent large-scale micropropagation
spellingShingle In vitro conservation of blackberry genotypes under minimal growth conditions and subsequent large-scale micropropagation
Gomes, Hugo Teixeira
Rubus; ex situ conservation; plant genetic resources; plant propagation; slow-growth
Rubus; conservação ex situ; recursos genéticos de plantas; propagação de plantas; crescimento lento
title_short In vitro conservation of blackberry genotypes under minimal growth conditions and subsequent large-scale micropropagation
title_full In vitro conservation of blackberry genotypes under minimal growth conditions and subsequent large-scale micropropagation
title_fullStr In vitro conservation of blackberry genotypes under minimal growth conditions and subsequent large-scale micropropagation
title_full_unstemmed In vitro conservation of blackberry genotypes under minimal growth conditions and subsequent large-scale micropropagation
title_sort In vitro conservation of blackberry genotypes under minimal growth conditions and subsequent large-scale micropropagation
author Gomes, Hugo Teixeira
author_facet Gomes, Hugo Teixeira
Bartos, Patrícia Monah Cunha
Andrade, Maíra Teixeira de
Almeida, Raphael Ferreira
Lacerda, Luciana Florencio de
Scherwinski-Pereira, Jonny Everson
author_role author
author2 Bartos, Patrícia Monah Cunha
Andrade, Maíra Teixeira de
Almeida, Raphael Ferreira
Lacerda, Luciana Florencio de
Scherwinski-Pereira, Jonny Everson
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Gomes, Hugo Teixeira
Bartos, Patrícia Monah Cunha
Andrade, Maíra Teixeira de
Almeida, Raphael Ferreira
Lacerda, Luciana Florencio de
Scherwinski-Pereira, Jonny Everson
dc.subject.por.fl_str_mv Rubus; ex situ conservation; plant genetic resources; plant propagation; slow-growth
Rubus; conservação ex situ; recursos genéticos de plantas; propagação de plantas; crescimento lento
topic Rubus; ex situ conservation; plant genetic resources; plant propagation; slow-growth
Rubus; conservação ex situ; recursos genéticos de plantas; propagação de plantas; crescimento lento
description The objective of this work was to evaluate the micropropagation of blackberry (Rubus spp.) cultivars, after in vitro conservation under minimal growth conditions. Nodal segments of the 'Guarani', 'Caingangue', 'Ébano', and 'Xavante' genotypes were conserved under minimal growth conditions at 20ºC, for 15 months. Microshoots were regenerated and multiplied by up to five successive subcultures, when they were rooted and acclimatized. After 30 days of acclimatization in a greenhouse, rooted plantlets showed no significant losses. Blackberry cultivars can be conserved in vitro for 15 months, without subcultures and, after this time, they can be micropropagated on a large-scale, maintaining the regenerative potential and multiplication.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-21
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/24722
url https://seer.sct.embrapa.br/index.php/pab/article/view/24722
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/24722/14052
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/24722/16555
dc.rights.driver.fl_str_mv Direitos autorais 2017 Pesquisa Agropecuária Brasileira
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Direitos autorais 2017 Pesquisa Agropecuária Brasileira
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.52, n.12, dez. 2017; 1286-1290
Pesquisa Agropecuária Brasileira; v.52, n.12, dez. 2017; 1286-1290
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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