Blood activity of glutathione peroxidase and its correlation with blood selenium concentration in grazing dairy cattle

Detalhes bibliográficos
Autor(a) principal: Ceballos, Alejandro
Data de Publicação: 1999
Outros Autores: Wittwer, Fernando German, Contreras, Pedro Alberto, Quiroz, Eduardo, Bohmwald, Helga Lidia
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/5573
Resumo: Selenium (Se) is part of the antioxidant enzyme glutathione peroxidase (GSH-Px; EC 1.11.1.9) structure, whose blood activity is related to the blood level of selenium. This study was designed to validate the analytical method to analyze the GSH-Px blood activity at the Clinical Pathology Laboratory of the Universidad Austral de Chile, and to correlate it with blood Se level in dairy cattle from the South of Chile. Blood heparinized samples were taken from 112 dairy cows from eight dairy herds located at Valdivia province, Chile. A kinetic NADPH-dependent technique was used to analyze the blood GSH-Px, and the content of Se in blood and plasma was analyzed by neutron activation. The Se concentration in blood was analyzed in 12 samples to correlate GSH-Px blood activity with blood and plasma Se level. The inaccuracy and imprecision were 5.9% and 10%, respectively. The mean and standard deviation of the obtained values were 89 ± 45 U/g of hemoglobin (Hb). The correlation between blood selenium and blood GSH-Px activity was r=0.97 (P < 0.05). Accordingly, it is possible to analyze the blood activity of GSH-Px by using the described technique. The blood activity of GSH-Px was related to the blood selenium level.
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spelling Blood activity of glutathione peroxidase and its correlation with blood selenium concentration in grazing dairy cattleActividad de glutatión peroxidasa en bovinos lecheros a pastoreo correlacionada con la concentración sanguinea y plasmática de selenioBlood activity of glutathione peroxidase and its correlation with blood selenium concentration in grazing dairy cattleantioxidants; oxidative stress; mineralsantioxidantes; estrés oxidativo; mineralesantioxidants; oxidative stress; mineralsSelenium (Se) is part of the antioxidant enzyme glutathione peroxidase (GSH-Px; EC 1.11.1.9) structure, whose blood activity is related to the blood level of selenium. This study was designed to validate the analytical method to analyze the GSH-Px blood activity at the Clinical Pathology Laboratory of the Universidad Austral de Chile, and to correlate it with blood Se level in dairy cattle from the South of Chile. Blood heparinized samples were taken from 112 dairy cows from eight dairy herds located at Valdivia province, Chile. A kinetic NADPH-dependent technique was used to analyze the blood GSH-Px, and the content of Se in blood and plasma was analyzed by neutron activation. The Se concentration in blood was analyzed in 12 samples to correlate GSH-Px blood activity with blood and plasma Se level. The inaccuracy and imprecision were 5.9% and 10%, respectively. The mean and standard deviation of the obtained values were 89 ± 45 U/g of hemoglobin (Hb). The correlation between blood selenium and blood GSH-Px activity was r=0.97 (P < 0.05). Accordingly, it is possible to analyze the blood activity of GSH-Px by using the described technique. The blood activity of GSH-Px was related to the blood selenium level.Con el objeto de validar una técnica para determinar la actividad sanguínea de glutatión peroxidasa (GSH-Px; EC 1.11.1.9) en el Laboratorio de Patología Clínica de la Universidad Austral de Chile y establecer la correlación entre su actividad y la concentración sanguínea y plasmática de selenio (Se) en bovinos a pastoreo en rebaños lecheros del sur de Chile, se tomaron 5-10 mL de sangre heparinizada a 112 vacas de ocho rebaños en la provincia de Valdivia. La actividad enzimática se analizó mediante una técnica cinética, y el Se por activación de neutrones. Fueron calculadas la inexactitud e imprecisión de la técnica cinética y se describen el rango, promedio y desviación estándar de la actividad enzimática. La correlación entre la actividad sanguínea de GSH-Px y la concentración de Se fue obtenida mediante el coeficiente de correlación simple. La inexactitud e imprecisión fueron 5,9% y 10%, respectivamente. La actividad de GSH-Px fue 89 ± 45 U/g de hemoglobina (Hb) y la correlación entre las variables señaladas fue r=0,97 (P < 0,05). Según estos resultados, es posible recomendar el uso rutinario de la técnica descrita. La correlación señalada permite anotar que en bovinos a pastoreo la actividad de GSH-Px está relacionada con la concentración sanguínea de selenio.Selenium (Se) is part of the antioxidant enzyme glutathione peroxidase (GSH-Px; EC 1.11.1.9) structure, whose blood activity is related to the blood level of selenium. This study was designed to validate the analytical method to analyze the GSH-Px blood activity at the Clinical Pathology Laboratory of the Universidad Austral de Chile, and to correlate it with blood Se level in dairy cattle from the South of Chile. Blood heparinized samples were taken from 112 dairy cows from eight dairy herds located at Valdivia province, Chile. A kinetic NADPH-dependent technique was used to analyze the blood GSH-Px, and the content of Se in blood and plasma was analyzed by neutron activation. The Se concentration in blood was analyzed in 12 samples to correlate GSH-Px blood activity with blood and plasma Se level. The inaccuracy and imprecision were 5.9% and 10%, respectively. The mean and standard deviation of the obtained values were 89 ± 45 U/g of hemoglobin (Hb). The correlation between blood selenium and blood GSH-Px activity was r=0.97 (P< 0.05). Accordingly, it is possible to analyze the blood activity of GSH-Px by using the described technique. The blood activity of GSH-Px was related to the blood selenium level.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCeballos, AlejandroWittwer, Fernando GermanContreras, Pedro AlbertoQuiroz, EduardoBohmwald, Helga Lidia1999-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/5573Pesquisa Agropecuaria Brasileira; v.34, n.12, dez. 1999; 2331-2338Pesquisa Agropecuária Brasileira; v.34, n.12, dez. 1999; 2331-23381678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/5573/2764info:eu-repo/semantics/openAccess2010-06-29T18:13:38Zoai:ojs.seer.sct.embrapa.br:article/5573Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2010-06-29T18:13:38Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Blood activity of glutathione peroxidase and its correlation with blood selenium concentration in grazing dairy cattle
Actividad de glutatión peroxidasa en bovinos lecheros a pastoreo correlacionada con la concentración sanguinea y plasmática de selenio
Blood activity of glutathione peroxidase and its correlation with blood selenium concentration in grazing dairy cattle
title Blood activity of glutathione peroxidase and its correlation with blood selenium concentration in grazing dairy cattle
spellingShingle Blood activity of glutathione peroxidase and its correlation with blood selenium concentration in grazing dairy cattle
Ceballos, Alejandro
antioxidants; oxidative stress; minerals
antioxidantes; estrés oxidativo; minerales
antioxidants; oxidative stress; minerals
title_short Blood activity of glutathione peroxidase and its correlation with blood selenium concentration in grazing dairy cattle
title_full Blood activity of glutathione peroxidase and its correlation with blood selenium concentration in grazing dairy cattle
title_fullStr Blood activity of glutathione peroxidase and its correlation with blood selenium concentration in grazing dairy cattle
title_full_unstemmed Blood activity of glutathione peroxidase and its correlation with blood selenium concentration in grazing dairy cattle
title_sort Blood activity of glutathione peroxidase and its correlation with blood selenium concentration in grazing dairy cattle
author Ceballos, Alejandro
author_facet Ceballos, Alejandro
Wittwer, Fernando German
Contreras, Pedro Alberto
Quiroz, Eduardo
Bohmwald, Helga Lidia
author_role author
author2 Wittwer, Fernando German
Contreras, Pedro Alberto
Quiroz, Eduardo
Bohmwald, Helga Lidia
author2_role author
author
author
author
dc.contributor.none.fl_str_mv


dc.contributor.author.fl_str_mv Ceballos, Alejandro
Wittwer, Fernando German
Contreras, Pedro Alberto
Quiroz, Eduardo
Bohmwald, Helga Lidia
dc.subject.por.fl_str_mv antioxidants; oxidative stress; minerals
antioxidantes; estrés oxidativo; minerales
antioxidants; oxidative stress; minerals
topic antioxidants; oxidative stress; minerals
antioxidantes; estrés oxidativo; minerales
antioxidants; oxidative stress; minerals
description Selenium (Se) is part of the antioxidant enzyme glutathione peroxidase (GSH-Px; EC 1.11.1.9) structure, whose blood activity is related to the blood level of selenium. This study was designed to validate the analytical method to analyze the GSH-Px blood activity at the Clinical Pathology Laboratory of the Universidad Austral de Chile, and to correlate it with blood Se level in dairy cattle from the South of Chile. Blood heparinized samples were taken from 112 dairy cows from eight dairy herds located at Valdivia province, Chile. A kinetic NADPH-dependent technique was used to analyze the blood GSH-Px, and the content of Se in blood and plasma was analyzed by neutron activation. The Se concentration in blood was analyzed in 12 samples to correlate GSH-Px blood activity with blood and plasma Se level. The inaccuracy and imprecision were 5.9% and 10%, respectively. The mean and standard deviation of the obtained values were 89 ± 45 U/g of hemoglobin (Hb). The correlation between blood selenium and blood GSH-Px activity was r=0.97 (P < 0.05). Accordingly, it is possible to analyze the blood activity of GSH-Px by using the described technique. The blood activity of GSH-Px was related to the blood selenium level.
publishDate 1999
dc.date.none.fl_str_mv 1999-12-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/5573
url https://seer.sct.embrapa.br/index.php/pab/article/view/5573
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/5573/2764
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.34, n.12, dez. 1999; 2331-2338
Pesquisa Agropecuária Brasileira; v.34, n.12, dez. 1999; 2331-2338
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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