Intra- and intergenomic chromosomal pairing in artificially polyploidized elephant grass and pearl millet hybrids

Detalhes bibliográficos
Autor(a) principal: Santos, Fernanda Motta da Costa
Data de Publicação: 2017
Outros Autores: Torres, Giovana Augusta, Techio, Vânia Helena, Pereira, Antônio Vander, Davide, Lisete Chamma
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/24975
Resumo: The objective of this work was to evaluate, by genomic in situ hybridization (GISH), pairing configurations as potential indicators of recombination between chromosomes of different parental genomes, in two interspecific hybrids (elephant grass x pearl millet) artificially polyploidized. Anthers from young flower buds were used in the chromosomal preparations. The genomic probe was prepared with pearl millet DNA and labeled with biotin-16-dUTP by the nick translation reaction. Blocking DNA was prepared with genomic elephant grass DNA. The homoeologous intergenomic pairing, observed in the two hybrids, indicates the possibility of recombination between chromosomes of the parental genomes.
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spelling Intra- and intergenomic chromosomal pairing in artificially polyploidized elephant grass and pearl millet hybridsPareamento cromossômico intra e intergenômico em híbridos de capim-elefante e milheto poliploidizados artificialmentePennisetum glaucum; Pennisetum purpureum; in situ hybridization; intergenomic pairing; interspecific hybridizationPennisetum glaucum; Pennisetum purpureum; hibridização in situ; pareamento intergenômico; hibridação interespecíficaThe objective of this work was to evaluate, by genomic in situ hybridization (GISH), pairing configurations as potential indicators of recombination between chromosomes of different parental genomes, in two interspecific hybrids (elephant grass x pearl millet) artificially polyploidized. Anthers from young flower buds were used in the chromosomal preparations. The genomic probe was prepared with pearl millet DNA and labeled with biotin-16-dUTP by the nick translation reaction. Blocking DNA was prepared with genomic elephant grass DNA. The homoeologous intergenomic pairing, observed in the two hybrids, indicates the possibility of recombination between chromosomes of the parental genomes.O objetivo deste trabalho foi avaliar, por meio da hibridização in situ genômica (GISH), as configurações de pareamento como indicadoras potenciais da recombinação entre cromossomos de diferentes genomas parentais, presentes em dois híbridos interespecíficos (capim-elefante x milheto) poliploidizados artificialmente. Anteras de botões florais jovens foram utilizadas nas preparações cromossômicas. A sonda genômica foi preparada com o DNA do milheto e marcada com biotina-16-dUTP pela reação de nick translation. O DNA de bloqueio foi preparado com o DNA genômico do capim-elefante. O pareamento intergenômico homeólogo, observado nos dois híbridos, indica a possibilidade de recombinação entre os cromossomos dos genomas parentais.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig)Juliane Dornelas Nunes, Embrapa Gado de Leite.Santos, Fernanda Motta da CostaTorres, Giovana AugustaTechio, Vânia HelenaPereira, Antônio VanderDavide, Lisete Chamma2017-10-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/24975Pesquisa Agropecuaria Brasileira; v.52, n.9, set. 2017; 814-817Pesquisa Agropecuária Brasileira; v.52, n.9, set. 2017; 814-8171678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/24975/13921https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/24975/16804https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/24975/16821Direitos autorais 2017 Pesquisa Agropecuária Brasileirainfo:eu-repo/semantics/openAccess2017-10-11T18:20:35Zoai:ojs.seer.sct.embrapa.br:article/24975Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2017-10-11T18:20:35Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Intra- and intergenomic chromosomal pairing in artificially polyploidized elephant grass and pearl millet hybrids
Pareamento cromossômico intra e intergenômico em híbridos de capim-elefante e milheto poliploidizados artificialmente
title Intra- and intergenomic chromosomal pairing in artificially polyploidized elephant grass and pearl millet hybrids
spellingShingle Intra- and intergenomic chromosomal pairing in artificially polyploidized elephant grass and pearl millet hybrids
Santos, Fernanda Motta da Costa
Pennisetum glaucum; Pennisetum purpureum; in situ hybridization; intergenomic pairing; interspecific hybridization
Pennisetum glaucum; Pennisetum purpureum; hibridização in situ; pareamento intergenômico; hibridação interespecífica
title_short Intra- and intergenomic chromosomal pairing in artificially polyploidized elephant grass and pearl millet hybrids
title_full Intra- and intergenomic chromosomal pairing in artificially polyploidized elephant grass and pearl millet hybrids
title_fullStr Intra- and intergenomic chromosomal pairing in artificially polyploidized elephant grass and pearl millet hybrids
title_full_unstemmed Intra- and intergenomic chromosomal pairing in artificially polyploidized elephant grass and pearl millet hybrids
title_sort Intra- and intergenomic chromosomal pairing in artificially polyploidized elephant grass and pearl millet hybrids
author Santos, Fernanda Motta da Costa
author_facet Santos, Fernanda Motta da Costa
Torres, Giovana Augusta
Techio, Vânia Helena
Pereira, Antônio Vander
Davide, Lisete Chamma
author_role author
author2 Torres, Giovana Augusta
Techio, Vânia Helena
Pereira, Antônio Vander
Davide, Lisete Chamma
author2_role author
author
author
author
dc.contributor.none.fl_str_mv
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig)
Juliane Dornelas Nunes, Embrapa Gado de Leite.
dc.contributor.author.fl_str_mv Santos, Fernanda Motta da Costa
Torres, Giovana Augusta
Techio, Vânia Helena
Pereira, Antônio Vander
Davide, Lisete Chamma
dc.subject.por.fl_str_mv Pennisetum glaucum; Pennisetum purpureum; in situ hybridization; intergenomic pairing; interspecific hybridization
Pennisetum glaucum; Pennisetum purpureum; hibridização in situ; pareamento intergenômico; hibridação interespecífica
topic Pennisetum glaucum; Pennisetum purpureum; in situ hybridization; intergenomic pairing; interspecific hybridization
Pennisetum glaucum; Pennisetum purpureum; hibridização in situ; pareamento intergenômico; hibridação interespecífica
description The objective of this work was to evaluate, by genomic in situ hybridization (GISH), pairing configurations as potential indicators of recombination between chromosomes of different parental genomes, in two interspecific hybrids (elephant grass x pearl millet) artificially polyploidized. Anthers from young flower buds were used in the chromosomal preparations. The genomic probe was prepared with pearl millet DNA and labeled with biotin-16-dUTP by the nick translation reaction. Blocking DNA was prepared with genomic elephant grass DNA. The homoeologous intergenomic pairing, observed in the two hybrids, indicates the possibility of recombination between chromosomes of the parental genomes.
publishDate 2017
dc.date.none.fl_str_mv 2017-10-11
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/24975
url https://seer.sct.embrapa.br/index.php/pab/article/view/24975
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/24975/13921
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/24975/16804
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/24975/16821
dc.rights.driver.fl_str_mv Direitos autorais 2017 Pesquisa Agropecuária Brasileira
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Direitos autorais 2017 Pesquisa Agropecuária Brasileira
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.52, n.9, set. 2017; 814-817
Pesquisa Agropecuária Brasileira; v.52, n.9, set. 2017; 814-817
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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