Molecular markers from coffee genome expressed sequences potentially involved in resistance to rust

Detalhes bibliográficos
Autor(a) principal: Alvarenga, Samuel Mazzinghy
Data de Publicação: 2011
Outros Autores: Caixeta, Eveline Teixeira, Hufnagel, Bárbara, Thiebaut, Flávia, Maciel-Zambolim, Eunize, Zambolim, Laércio, Sakiyama, Ney Sussumu
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/10611
Resumo: The objective of this work was to identify molecular markers related to the resistance of coffee (Coffea arabica) to rust (Hemileia vastatrix). DNA sequences potentially involved in coffee disease resistance were identified, using “in silico” analysis, from data obtained by the Brazilian coffee genome project. After data mining, 59 primer pairs were designed to amplify the sequences identified. The 59 primers were tested on 12 resistant and 12 susceptible coffee plants to H. vastatrix. Twenty-seven primers resulted in unique and well‑defined bands, while one of these amplified a DNA fragment in all resistant plants, but not in the susceptible ones. This polymorphic molecular marker amplified a region of DNA that corresponds to a partial open reading frame of C. arabica genome that encodes a disease resistance protein. The marker CARF 005 can be used to differentiate between resistant and susceptible coffee plants to H. vastatrix.
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spelling Molecular markers from coffee genome expressed sequences potentially involved in resistance to rustMarcadores moleculares derivados de sequências expressas do genoma café potencialmente envolvidas na resistência à ferrugemCoffea arabica; Hemileia vastatrix; bioinformatics; EST-PCR; resistance genes; data miningCoffea arabica; Hemileia vastatrix; bioinformática; EST-PCR; genes de resistência; mineração de dadosThe objective of this work was to identify molecular markers related to the resistance of coffee (Coffea arabica) to rust (Hemileia vastatrix). DNA sequences potentially involved in coffee disease resistance were identified, using “in silico” analysis, from data obtained by the Brazilian coffee genome project. After data mining, 59 primer pairs were designed to amplify the sequences identified. The 59 primers were tested on 12 resistant and 12 susceptible coffee plants to H. vastatrix. Twenty-seven primers resulted in unique and well‑defined bands, while one of these amplified a DNA fragment in all resistant plants, but not in the susceptible ones. This polymorphic molecular marker amplified a region of DNA that corresponds to a partial open reading frame of C. arabica genome that encodes a disease resistance protein. The marker CARF 005 can be used to differentiate between resistant and susceptible coffee plants to H. vastatrix.O objetivo deste trabalho foi identificar marcadores moleculares relacionados à resistência do cafeeiro (Coffea arabica) à ferrugem (Hemileia vastatrix). Foram identificadas sequências de DNA potencialmente envolvidas na resistência do cafeeiro a doenças, por meio de análise “in silico”, a partir das informações geradas pelo Projeto Brasileiro do Genoma Café. A partir das sequências mineradas, foram desenhados 59 pares de iniciadores para amplificá‑las. Os 59 iniciadores foram testados em 12 cafeeiros resistentes e 12 susceptíveis a H. vastatrix. Vinte e sete iniciadores resultaram em bandas únicas e bem definidas, enquanto um deles amplificou fragmento de DNA em todos os cafeeiros resistentes, mas não nos suscetíveis. Esse marcador molecular polimórfico amplificou uma região do DNA que corresponde a uma janela aberta de leitura parcial do genoma de C. arabica que codifica uma proteína de resistência a doenças. O marcador CARF 005 é capaz de diferenciar os cafeeiros analisados em resistentes e susceptíveis a H. vastatrix.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCNPqCBP&D/CaféFINEPAlvarenga, Samuel MazzinghyCaixeta, Eveline TeixeiraHufnagel, BárbaraThiebaut, FláviaMaciel-Zambolim, EunizeZambolim, LaércioSakiyama, Ney Sussumu2011-10-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/10611Pesquisa Agropecuaria Brasileira; v.46, n.8, ago. 2011; 890-898Pesquisa Agropecuária Brasileira; v.46, n.8, ago. 2011; 890-8981678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/10611/6495https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/10611/6005info:eu-repo/semantics/openAccess2014-06-05T17:50:05Zoai:ojs.seer.sct.embrapa.br:article/10611Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-06-05T17:50:05Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Molecular markers from coffee genome expressed sequences potentially involved in resistance to rust
Marcadores moleculares derivados de sequências expressas do genoma café potencialmente envolvidas na resistência à ferrugem
title Molecular markers from coffee genome expressed sequences potentially involved in resistance to rust
spellingShingle Molecular markers from coffee genome expressed sequences potentially involved in resistance to rust
Alvarenga, Samuel Mazzinghy
Coffea arabica; Hemileia vastatrix; bioinformatics; EST-PCR; resistance genes; data mining
Coffea arabica; Hemileia vastatrix; bioinformática; EST-PCR; genes de resistência; mineração de dados
title_short Molecular markers from coffee genome expressed sequences potentially involved in resistance to rust
title_full Molecular markers from coffee genome expressed sequences potentially involved in resistance to rust
title_fullStr Molecular markers from coffee genome expressed sequences potentially involved in resistance to rust
title_full_unstemmed Molecular markers from coffee genome expressed sequences potentially involved in resistance to rust
title_sort Molecular markers from coffee genome expressed sequences potentially involved in resistance to rust
author Alvarenga, Samuel Mazzinghy
author_facet Alvarenga, Samuel Mazzinghy
Caixeta, Eveline Teixeira
Hufnagel, Bárbara
Thiebaut, Flávia
Maciel-Zambolim, Eunize
Zambolim, Laércio
Sakiyama, Ney Sussumu
author_role author
author2 Caixeta, Eveline Teixeira
Hufnagel, Bárbara
Thiebaut, Flávia
Maciel-Zambolim, Eunize
Zambolim, Laércio
Sakiyama, Ney Sussumu
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv
CNPq
CBP&D/Café
FINEP
dc.contributor.author.fl_str_mv Alvarenga, Samuel Mazzinghy
Caixeta, Eveline Teixeira
Hufnagel, Bárbara
Thiebaut, Flávia
Maciel-Zambolim, Eunize
Zambolim, Laércio
Sakiyama, Ney Sussumu
dc.subject.por.fl_str_mv Coffea arabica; Hemileia vastatrix; bioinformatics; EST-PCR; resistance genes; data mining
Coffea arabica; Hemileia vastatrix; bioinformática; EST-PCR; genes de resistência; mineração de dados
topic Coffea arabica; Hemileia vastatrix; bioinformatics; EST-PCR; resistance genes; data mining
Coffea arabica; Hemileia vastatrix; bioinformática; EST-PCR; genes de resistência; mineração de dados
description The objective of this work was to identify molecular markers related to the resistance of coffee (Coffea arabica) to rust (Hemileia vastatrix). DNA sequences potentially involved in coffee disease resistance were identified, using “in silico” analysis, from data obtained by the Brazilian coffee genome project. After data mining, 59 primer pairs were designed to amplify the sequences identified. The 59 primers were tested on 12 resistant and 12 susceptible coffee plants to H. vastatrix. Twenty-seven primers resulted in unique and well‑defined bands, while one of these amplified a DNA fragment in all resistant plants, but not in the susceptible ones. This polymorphic molecular marker amplified a region of DNA that corresponds to a partial open reading frame of C. arabica genome that encodes a disease resistance protein. The marker CARF 005 can be used to differentiate between resistant and susceptible coffee plants to H. vastatrix.
publishDate 2011
dc.date.none.fl_str_mv 2011-10-26
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/10611
url https://seer.sct.embrapa.br/index.php/pab/article/view/10611
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/10611/6495
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/10611/6005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.46, n.8, ago. 2011; 890-898
Pesquisa Agropecuária Brasileira; v.46, n.8, ago. 2011; 890-898
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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