Methodology for sporulation and production of monoconidial isolates of Sphaeropsis sapinea

Detalhes bibliográficos
Autor(a) principal: Basilio, Paula Rachel Rabelo Corrêa
Data de Publicação: 2010
Outros Autores: Auer, Celso Garcia, Santos, Álvaro Figueredo dos, Higa, Antonio Rioyei
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Florestal Brasileira (Online)
Texto Completo: https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/139
Resumo: Sphaeropsis sapinea is one of main pathogens that causes losses in commercial plantations of Pinusspp. Studies for control based on genetic resistance can be done by inoculating different monosporic isolates onpine populations and selectioning the resistant individuals. This work describes a methodology for pycnidia production in vitro and S. sapinea monoconidial cultures, using an agar-water medium with sterile pine needles. Four S. sapinea isolates were tested e pycnidia and mature conidia were observed after 14 days of incubation, under continuous fluorescent light, in BOD, at 25 °C. Pycnidia were triturated in sterile water and conidia germinated after two hours of incubation in water-agar, in BOD, at 25 °C. Germinated conidia formed colonies which were purified in malt extract-agar and maintained in test tubes with the same medium, covered with sterile mineral oil for preservation. This methodology revealed to be  adequated for production of monoconidial coloniesof S. sapinea
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spelling Methodology for sporulation and production of monoconidial isolates of Sphaeropsis sapineaMetodologia para esporulação e produção de culturas monospóricas de Sphaeropsis sapineaInóculopatógenopínus.Inoculumpathogenpine.Sphaeropsis sapinea is one of main pathogens that causes losses in commercial plantations of Pinusspp. Studies for control based on genetic resistance can be done by inoculating different monosporic isolates onpine populations and selectioning the resistant individuals. This work describes a methodology for pycnidia production in vitro and S. sapinea monoconidial cultures, using an agar-water medium with sterile pine needles. Four S. sapinea isolates were tested e pycnidia and mature conidia were observed after 14 days of incubation, under continuous fluorescent light, in BOD, at 25 °C. Pycnidia were triturated in sterile water and conidia germinated after two hours of incubation in water-agar, in BOD, at 25 °C. Germinated conidia formed colonies which were purified in malt extract-agar and maintained in test tubes with the same medium, covered with sterile mineral oil for preservation. This methodology revealed to be  adequated for production of monoconidial coloniesof S. sapineaSphaeropsis sapinea é um dos principais patógenos causadores de perdas em plantios comerciais de Pinus spp. Estudos de controle por resistência genética podem ser feitos pela inoculação de vários isolados monospóricos em populações de pínus e selecionando-se os indivíduos resistentes. Este trabalho descreve uma metodologia de produção de picnídios in vitro e culturas monospóricas de S. sapinea, usando um meio ágarágua (AA) com fragmentos de acículas de Pinus taeda esterilizadas. Quatro isolados de S. sapinea foram testados e os picnídios e conídios maduros foram observados após 14 dias de incubação, sob luz fria em BOD, a 25 °C. Os picnídios foram triturados em água e os conídios germinaram após duas horas de incubação em meio AA, em BOD, a 25 ºC. Os conídios germinados formaram  colônias que foram purificadas em meio extrato de malteágar e mantidas em tubos de ensaio com o mesmo meio e imersas em óleo mineral estéril para preservação. A metodologia revelou ser adequada para a produção de colônias monospóricas do fungo S. sapinea.Embrapa Florestas2010-06-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/139Pesquisa Florestal Brasileira; n. 54 (2007): jan./jun. Pesquisa Florestal Brasileira; 145Pesquisa Florestal Brasileira; No. 54 (2007): jan./jun. Pesquisa Florestal Brasileira; 1451983-26051809-3647reponame:Pesquisa Florestal Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/139/99Basilio, Paula Rachel Rabelo CorrêaAuer, Celso GarciaSantos, Álvaro Figueredo dosHiga, Antonio Rioyeiinfo:eu-repo/semantics/openAccess2017-04-28T14:09:00Zoai:pfb.cnpf.embrapa.br/pfb:article/139Revistahttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/PUBhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/oaipfb@embrapa.br || revista.pfb@gmail.com || patricia.mattos@embrapa.br1983-26051809-3647opendoar:2017-04-28T14:09Pesquisa Florestal Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Methodology for sporulation and production of monoconidial isolates of Sphaeropsis sapinea
Metodologia para esporulação e produção de culturas monospóricas de Sphaeropsis sapinea
title Methodology for sporulation and production of monoconidial isolates of Sphaeropsis sapinea
spellingShingle Methodology for sporulation and production of monoconidial isolates of Sphaeropsis sapinea
Basilio, Paula Rachel Rabelo Corrêa
Inóculo
patógeno
pínus.
Inoculum
pathogen
pine.
title_short Methodology for sporulation and production of monoconidial isolates of Sphaeropsis sapinea
title_full Methodology for sporulation and production of monoconidial isolates of Sphaeropsis sapinea
title_fullStr Methodology for sporulation and production of monoconidial isolates of Sphaeropsis sapinea
title_full_unstemmed Methodology for sporulation and production of monoconidial isolates of Sphaeropsis sapinea
title_sort Methodology for sporulation and production of monoconidial isolates of Sphaeropsis sapinea
author Basilio, Paula Rachel Rabelo Corrêa
author_facet Basilio, Paula Rachel Rabelo Corrêa
Auer, Celso Garcia
Santos, Álvaro Figueredo dos
Higa, Antonio Rioyei
author_role author
author2 Auer, Celso Garcia
Santos, Álvaro Figueredo dos
Higa, Antonio Rioyei
author2_role author
author
author
dc.contributor.author.fl_str_mv Basilio, Paula Rachel Rabelo Corrêa
Auer, Celso Garcia
Santos, Álvaro Figueredo dos
Higa, Antonio Rioyei
dc.subject.por.fl_str_mv Inóculo
patógeno
pínus.
Inoculum
pathogen
pine.
topic Inóculo
patógeno
pínus.
Inoculum
pathogen
pine.
description Sphaeropsis sapinea is one of main pathogens that causes losses in commercial plantations of Pinusspp. Studies for control based on genetic resistance can be done by inoculating different monosporic isolates onpine populations and selectioning the resistant individuals. This work describes a methodology for pycnidia production in vitro and S. sapinea monoconidial cultures, using an agar-water medium with sterile pine needles. Four S. sapinea isolates were tested e pycnidia and mature conidia were observed after 14 days of incubation, under continuous fluorescent light, in BOD, at 25 °C. Pycnidia were triturated in sterile water and conidia germinated after two hours of incubation in water-agar, in BOD, at 25 °C. Germinated conidia formed colonies which were purified in malt extract-agar and maintained in test tubes with the same medium, covered with sterile mineral oil for preservation. This methodology revealed to be  adequated for production of monoconidial coloniesof S. sapinea
publishDate 2010
dc.date.none.fl_str_mv 2010-06-28
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/139
url https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/139
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/139/99
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Embrapa Florestas
publisher.none.fl_str_mv Embrapa Florestas
dc.source.none.fl_str_mv Pesquisa Florestal Brasileira; n. 54 (2007): jan./jun. Pesquisa Florestal Brasileira; 145
Pesquisa Florestal Brasileira; No. 54 (2007): jan./jun. Pesquisa Florestal Brasileira; 145
1983-2605
1809-3647
reponame:Pesquisa Florestal Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Florestal Brasileira (Online)
collection Pesquisa Florestal Brasileira (Online)
repository.name.fl_str_mv Pesquisa Florestal Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pfb@embrapa.br || revista.pfb@gmail.com || patricia.mattos@embrapa.br
_version_ 1783370932458356736

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