Growth regulators on callogenesis and rhizogenesis in Eugenia involucrata

• Autor(a) principal: Golle, Diego Pascoal
• Data de Publicação: 2020
• Outros Autores: Reiniger, Lia Rejane Silveira, Stefanel, Charlene Moro, Serrote, Caetano Miguel Lemos
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Pesquisa Florestal Brasileira (Online)
• Texto Completo: https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1908
• topic: Biotecnologia vegetal; Organogênese; Propagação vegetativa; Plant biotechnology; Organogenesis; Vegetative propagation

This study evaluated the effect of growth regulators on callogenesis and rhizogenesis in Eugenia involucrata. In two experiments, we tested the naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP), and 2,4-dichlorophenoxyacetic acid (2,4-D) and BAP associations. After 45 days of culture, all treatments formed calli, however, those treatments with NAA (alone or combined with BAP) and 2,4-D combined with BAP (5:5 and 10:10 μM) presented the best results. Only the use of NAA at 10 μM induced rhizogenesis. At 90 days of culture, the effect of phytoregulators was most evident, where 2,4-D combined with BAP (5:5 and 10:10 μM) were more favorable for callogenesis while 10 μM NAA were more favorable for rhizogenesis. These results indicate that cell dedifferentiation and re-differentiation processes are achievable from leaf explants of Eugenia involucrata.