Surface sterilization effect on germination and seeds incidence of fungi of forest species

• Autor(a) principal: Garrido Pinheiro, Carlos
• Data de Publicação: 2016
• Outros Autores: Lazarotto, Marília, Brião Muniz, Marlove Fatima, Gouvêa Redin, Cristina, Velho dos Santos, Mateus
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Pesquisa Florestal Brasileira (Online)
• Texto Completo: https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1234
• topic: Forest seed; Forest health; Seedlings; Semente florestal; Sanidade florestal; Plântulas

The aim of this study was to test treatments for sterilization and its influence on germination and health of seeds and seedlings of four forest species: Bauhinia forficata, Cedrela fissilis, Parapiptadenia rigida and Senegalia bonariensis. The seeds were subjected to the following treatments: control without treatment (T0); sodium hypochlorite 1% for 3 min (T1); sodium hypochlorite 2% for 1 min + one rinse with distilled water (T2); alcohol 70% for 1 min + triple rinse with distilled water + sodium hypochlorite 0.5% for 1 min + triple rinse with distilled water (T3); Lysoform ® for 1 min (T4); alcohol 70% for 1 min + sodium hypochlorite 1% for 1 min + one rinse with distilled water for 1 min (T5). Seeds were subjected to germination tests and we evaluate length of seedlings, fresh and dry weight, and sanity test. T2 is recommended for B. forficata presenting increase on germination potential and on seedling length; the same treatment is recommended for C. fissilis to increase germination and reduction of Fusarium sp. incidence; all aseptic treatments reduced incidence of P. rigida storage pathogen, however, there was no influence on germination; and seeds of S. bonariensis do not need to be sterilized. The recommended surface sterilization treatments could be routinely used in seeds of the studied species.