Adjustment methodology of the tetrazolium test for viability evaluation of angico seeds

Detalhes bibliográficos
Autor(a) principal: Pinho, Daniel Santos
Data de Publicação: 2011
Outros Autores: Borges, Eduardo Euclydes de Lima, Carvalho, Ana Paula Vilela, Corte, Viviana Borges
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Florestal Brasileira (Online)
Texto Completo: https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/197
Resumo: The aim of this experiment was to evaluate the best concentration  and the incubation time to test germination in seeds of  Anadenanthera peregrina (L.) Speg. The seeds were  preconditioned at 25 ºC for 14 hours immersed in distilled water.  The coat were removed and the embryos submerged in tetrazolium solution (pH 6.5) solution in the concentrations of  0.1%; 0.05% and 0.01%, being kept in dark at 25 ºC for three and five hours and at 30 ºC for 24 hours. It was evaluate the intensity and localization of the coloration, presence of milky areas, tissues aspect. The embryos were classified in nine classes of viability. The best treatment as substitute of the germination test was the 0.1% tetrazolium incubated at 30 ºC for 24 hours. Viable seeds of A. peregrine were classified in classes 1, 2 and 3.doi: 10.4336/2011.pfb.31.67.269
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spelling Adjustment methodology of the tetrazolium test for viability evaluation of angico seedsAdequação da metodologia do teste de tetrazólio para avaliação da viabilidade de sementes de angicoPhysiological qualityGerminationAnadenanthera peregrinaQualidadefisiológica GerminaçãoAnadenanthera peregrinaThe aim of this experiment was to evaluate the best concentration  and the incubation time to test germination in seeds of  Anadenanthera peregrina (L.) Speg. The seeds were  preconditioned at 25 ºC for 14 hours immersed in distilled water.  The coat were removed and the embryos submerged in tetrazolium solution (pH 6.5) solution in the concentrations of  0.1%; 0.05% and 0.01%, being kept in dark at 25 ºC for three and five hours and at 30 ºC for 24 hours. It was evaluate the intensity and localization of the coloration, presence of milky areas, tissues aspect. The embryos were classified in nine classes of viability. The best treatment as substitute of the germination test was the 0.1% tetrazolium incubated at 30 ºC for 24 hours. Viable seeds of A. peregrine were classified in classes 1, 2 and 3.doi: 10.4336/2011.pfb.31.67.269 Este trabalho teve por objetivo adequar a metodologia do teste em sementes de Anadenanthera peregrina (L.) Speg. As sementes foram pré-condicionadas por imersão em água destilada a 25 ºC por 14 horas. O tegumento foi retirado e os embriões submersos em solução de tetrazólio (pH 6,5) nas concentrações de 0,1%; 0,05% e 0,01%, sendo mantidos no escuro a 25 ºC por três e cinco horas e a 30 ºC por 24 horas. Avaliaram-se a intensidade e localização da coloração, presença de áreas leitosas e aspecto dos tecidos. Os embriões foram classificados em nove classes de viabilidade. O melhor tratamento como substituto do teste padrão de germinação foi a concentração de 0,1% e a incubação a 30 ºC por 24 horas. Sementes viáveis de A. peregrina foram classificadas nas classes 1, 2 e 3.doi: 10.4336/2011.pfb.31.67.269Embrapa Florestas2011-08-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/197Pesquisa Florestal Brasileira; v. 31 n. 67 (2011): jul./set.; 269Pesquisa Florestal Brasileira; Vol. 31 No. 67 (2011): jul./set.; 2691983-26051809-3647reponame:Pesquisa Florestal Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/197/226Pinho, Daniel SantosBorges, Eduardo Euclydes de LimaCarvalho, Ana Paula VilelaCorte, Viviana Borgesinfo:eu-repo/semantics/openAccess2017-04-28T13:06:26Zoai:pfb.cnpf.embrapa.br/pfb:article/197Revistahttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/PUBhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/oaipfb@embrapa.br || revista.pfb@gmail.com || patricia.mattos@embrapa.br1983-26051809-3647opendoar:2017-04-28T13:06:26Pesquisa Florestal Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Adjustment methodology of the tetrazolium test for viability evaluation of angico seeds
Adequação da metodologia do teste de tetrazólio para avaliação da viabilidade de sementes de angico
title Adjustment methodology of the tetrazolium test for viability evaluation of angico seeds
spellingShingle Adjustment methodology of the tetrazolium test for viability evaluation of angico seeds
Pinho, Daniel Santos
Physiological quality
Germination
Anadenanthera peregrina
Qualidade
fisiológica Germinação
Anadenanthera peregrina
title_short Adjustment methodology of the tetrazolium test for viability evaluation of angico seeds
title_full Adjustment methodology of the tetrazolium test for viability evaluation of angico seeds
title_fullStr Adjustment methodology of the tetrazolium test for viability evaluation of angico seeds
title_full_unstemmed Adjustment methodology of the tetrazolium test for viability evaluation of angico seeds
title_sort Adjustment methodology of the tetrazolium test for viability evaluation of angico seeds
author Pinho, Daniel Santos
author_facet Pinho, Daniel Santos
Borges, Eduardo Euclydes de Lima
Carvalho, Ana Paula Vilela
Corte, Viviana Borges
author_role author
author2 Borges, Eduardo Euclydes de Lima
Carvalho, Ana Paula Vilela
Corte, Viviana Borges
author2_role author
author
author
dc.contributor.author.fl_str_mv Pinho, Daniel Santos
Borges, Eduardo Euclydes de Lima
Carvalho, Ana Paula Vilela
Corte, Viviana Borges
dc.subject.por.fl_str_mv Physiological quality
Germination
Anadenanthera peregrina
Qualidade
fisiológica Germinação
Anadenanthera peregrina
topic Physiological quality
Germination
Anadenanthera peregrina
Qualidade
fisiológica Germinação
Anadenanthera peregrina
description The aim of this experiment was to evaluate the best concentration  and the incubation time to test germination in seeds of  Anadenanthera peregrina (L.) Speg. The seeds were  preconditioned at 25 ºC for 14 hours immersed in distilled water.  The coat were removed and the embryos submerged in tetrazolium solution (pH 6.5) solution in the concentrations of  0.1%; 0.05% and 0.01%, being kept in dark at 25 ºC for three and five hours and at 30 ºC for 24 hours. It was evaluate the intensity and localization of the coloration, presence of milky areas, tissues aspect. The embryos were classified in nine classes of viability. The best treatment as substitute of the germination test was the 0.1% tetrazolium incubated at 30 ºC for 24 hours. Viable seeds of A. peregrine were classified in classes 1, 2 and 3.doi: 10.4336/2011.pfb.31.67.269
publishDate 2011
dc.date.none.fl_str_mv 2011-08-26
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/197
url https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/197
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/197/226
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Embrapa Florestas
publisher.none.fl_str_mv Embrapa Florestas
dc.source.none.fl_str_mv Pesquisa Florestal Brasileira; v. 31 n. 67 (2011): jul./set.; 269
Pesquisa Florestal Brasileira; Vol. 31 No. 67 (2011): jul./set.; 269
1983-2605
1809-3647
reponame:Pesquisa Florestal Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Florestal Brasileira (Online)
collection Pesquisa Florestal Brasileira (Online)
repository.name.fl_str_mv Pesquisa Florestal Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pfb@embrapa.br || revista.pfb@gmail.com || patricia.mattos@embrapa.br
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