Amazonian açai and food dyes for staining arbuscular- micorrhizal fungi

Detalhes bibliográficos
Autor(a) principal: Silva, Aline Lourdes Martins
Data de Publicação: 2015
Outros Autores: Santana, Marcos Diones Ferreira, Pereira, John César de Jesus, Raimam, Milena Pupo, Albino, Ulisses Brigatto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Florestal Brasileira (Online)
Texto Completo: https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/798
Resumo: Arbuscular mycorrhizae microscopy requires differential staining of typical structures. Dyes employed, such as trypan blue, pose risks to health and environment. Alternative dyes such as pen ink and aniline have variable coloring efficiency. In this work, Brachiaria decumbens roots, discolored with caustic soda (NaOH), were stained with açai, annatto, saffron, trypan blue and pen inks. There were significant differences among dyes regarding stained mycorrhizal structures and pictures quality. Acai was considered the best alternative dye, with similar results to trypan blue.
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spelling Amazonian açai and food dyes for staining arbuscular- micorrhizal fungiAçaí da Amazônia como corante de fungos micorrízicos arbuscularesCorantes NaturaisMicrobiologia do soloTécnicaNatural dyesSoil microbiologyEuterpe oleraceaArbuscular mycorrhizae microscopy requires differential staining of typical structures. Dyes employed, such as trypan blue, pose risks to health and environment. Alternative dyes such as pen ink and aniline have variable coloring efficiency. In this work, Brachiaria decumbens roots, discolored with caustic soda (NaOH), were stained with açai, annatto, saffron, trypan blue and pen inks. There were significant differences among dyes regarding stained mycorrhizal structures and pictures quality. Acai was considered the best alternative dye, with similar results to trypan blue.A microscopia de Micorrizas Arbusculares requer coloração diferencial de estruturas típicas. Alguns corantes empregados, como Trypan Blue, oferecem riscos à saúde e ambiente. Corantes alternativos como tintas de caneta e anilina foram propostos, porém, com eficácia de coloração variável. Neste trabalho o descolorante de raízes KOH foi substituído por Soda Cáustica e foram testados os corantes naturais Açaí, Urucum e Açafrão, comparados a Trypan Blue e tintas de caneta. Houve diferença significativa entre os corantes quanto às estruturas micorrízicas coradas e qualidade de imagens fotografadas. Açaí foi considerado o melhor corante alternativo, com resultados similares ao Trypan Blue.Embrapa Florestas2015-12-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/79810.4336/2015.pfb.35.84.798Pesquisa Florestal Brasileira; v. 35 n. 84 (2015): out./dez.; 475-479Pesquisa Florestal Brasileira; Vol. 35 No. 84 (2015): out./dez.; 475-4791983-26051809-3647reponame:Pesquisa Florestal Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/798/460Copyright (c) 2015 Aline Lourdes Martins Silva, Marcos Diones Ferreira Santana, John César de Jesus Pereira, Milena Pupo Raimam, Ulisses Brigatto Albinohttps://creativecommons.org/licenses/by-nc-nd/4.0info:eu-repo/semantics/openAccessSilva, Aline Lourdes MartinsSantana, Marcos Diones FerreiraPereira, John César de JesusRaimam, Milena PupoAlbino, Ulisses Brigatto2017-11-15T17:19:34Zoai:pfb.cnpf.embrapa.br/pfb:article/798Revistahttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/PUBhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/oaipfb@embrapa.br || revista.pfb@gmail.com || patricia.mattos@embrapa.br1983-26051809-3647opendoar:2017-11-15T17:19:34Pesquisa Florestal Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Amazonian açai and food dyes for staining arbuscular- micorrhizal fungi
Açaí da Amazônia como corante de fungos micorrízicos arbusculares
title Amazonian açai and food dyes for staining arbuscular- micorrhizal fungi
spellingShingle Amazonian açai and food dyes for staining arbuscular- micorrhizal fungi
Silva, Aline Lourdes Martins
Corantes Naturais
Microbiologia do solo
Técnica
Natural dyes
Soil microbiology
Euterpe oleracea
title_short Amazonian açai and food dyes for staining arbuscular- micorrhizal fungi
title_full Amazonian açai and food dyes for staining arbuscular- micorrhizal fungi
title_fullStr Amazonian açai and food dyes for staining arbuscular- micorrhizal fungi
title_full_unstemmed Amazonian açai and food dyes for staining arbuscular- micorrhizal fungi
title_sort Amazonian açai and food dyes for staining arbuscular- micorrhizal fungi
author Silva, Aline Lourdes Martins
author_facet Silva, Aline Lourdes Martins
Santana, Marcos Diones Ferreira
Pereira, John César de Jesus
Raimam, Milena Pupo
Albino, Ulisses Brigatto
author_role author
author2 Santana, Marcos Diones Ferreira
Pereira, John César de Jesus
Raimam, Milena Pupo
Albino, Ulisses Brigatto
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Silva, Aline Lourdes Martins
Santana, Marcos Diones Ferreira
Pereira, John César de Jesus
Raimam, Milena Pupo
Albino, Ulisses Brigatto
dc.subject.por.fl_str_mv Corantes Naturais
Microbiologia do solo
Técnica
Natural dyes
Soil microbiology
Euterpe oleracea
topic Corantes Naturais
Microbiologia do solo
Técnica
Natural dyes
Soil microbiology
Euterpe oleracea
description Arbuscular mycorrhizae microscopy requires differential staining of typical structures. Dyes employed, such as trypan blue, pose risks to health and environment. Alternative dyes such as pen ink and aniline have variable coloring efficiency. In this work, Brachiaria decumbens roots, discolored with caustic soda (NaOH), were stained with açai, annatto, saffron, trypan blue and pen inks. There were significant differences among dyes regarding stained mycorrhizal structures and pictures quality. Acai was considered the best alternative dye, with similar results to trypan blue.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-31
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/798
10.4336/2015.pfb.35.84.798
url https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/798
identifier_str_mv 10.4336/2015.pfb.35.84.798
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/798/460
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Embrapa Florestas
publisher.none.fl_str_mv Embrapa Florestas
dc.source.none.fl_str_mv Pesquisa Florestal Brasileira; v. 35 n. 84 (2015): out./dez.; 475-479
Pesquisa Florestal Brasileira; Vol. 35 No. 84 (2015): out./dez.; 475-479
1983-2605
1809-3647
reponame:Pesquisa Florestal Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Florestal Brasileira (Online)
collection Pesquisa Florestal Brasileira (Online)
repository.name.fl_str_mv Pesquisa Florestal Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pfb@embrapa.br || revista.pfb@gmail.com || patricia.mattos@embrapa.br
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