Callus induction in vitro on leaves explants of Ilex paraguariensis
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Florestal Brasileira (Online) |
Texto Completo: | https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/441 |
Resumo: | Organogenesis is a technique rarely studied in the micro propagation of Ilex paraguariensis. This study evaluated different culture media on callus induction and in vitro organogenesis of this species. Leaves were collected from greenhouse grown plants. Leaves segments were placed on culture media ¼ MS, WPM or JADS, containing zeatin and 2,4-D. The MS medium was more efficient in callus inducing. Rooting was observed in the WPM medium. No buds were formed in any of the media evaluated. |
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Callus induction in vitro on leaves explants of Ilex paraguariensisEfeito do meio de cultura na calogênese in vitro a partir de folhas de erva-mateZeatin24-DRizogenesisOrganogenesisZeatina24-DRizogêneseOrganogêneseOrganogenesis is a technique rarely studied in the micro propagation of Ilex paraguariensis. This study evaluated different culture media on callus induction and in vitro organogenesis of this species. Leaves were collected from greenhouse grown plants. Leaves segments were placed on culture media ¼ MS, WPM or JADS, containing zeatin and 2,4-D. The MS medium was more efficient in callus inducing. Rooting was observed in the WPM medium. No buds were formed in any of the media evaluated.A organogênese é uma técnica pouco estudada na micropropagação de erva-mate. Este trabalho objetivou avaliar diferentes meios de cultura na calogênese in vitro e organogênese de Ilex paraguariensis St. Hil. Foram coletadas folhas em plantas em casa-de-vegetação. Segmentos foliares foram colocados em meios de cultura ¼ MS, WPM ou JADS, contendo zeatina e 2,4-D. O meio MS foi mais eficiente na indução de calos. No meio WPM foi observada rizogênese. Nos meios testados não houve formação de brotações adventícias.Embrapa Florestas2013-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/44110.4336/2013.pfb.33.75.441Pesquisa Florestal Brasileira; v. 33 n. 75 (2013): jul./set.; 339-342Pesquisa Florestal Brasileira; Vol. 33 No. 75 (2013): jul./set.; 339-3421983-26051809-3647reponame:Pesquisa Florestal Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/441/332Stachevski, Thamires WeigertFranciscon, LuzianeDegenhardt-Goldbach, Julianainfo:eu-repo/semantics/openAccess2017-04-28T12:50:27Zoai:pfb.cnpf.embrapa.br/pfb:article/441Revistahttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/PUBhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/oaipfb@embrapa.br || revista.pfb@gmail.com || patricia.mattos@embrapa.br1983-26051809-3647opendoar:2017-04-28T12:50:27Pesquisa Florestal Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Callus induction in vitro on leaves explants of Ilex paraguariensis Efeito do meio de cultura na calogênese in vitro a partir de folhas de erva-mate |
title |
Callus induction in vitro on leaves explants of Ilex paraguariensis |
spellingShingle |
Callus induction in vitro on leaves explants of Ilex paraguariensis Stachevski, Thamires Weigert Zeatin 2 4-D Rizogenesis Organogenesis Zeatina 2 4-D Rizogênese Organogênese |
title_short |
Callus induction in vitro on leaves explants of Ilex paraguariensis |
title_full |
Callus induction in vitro on leaves explants of Ilex paraguariensis |
title_fullStr |
Callus induction in vitro on leaves explants of Ilex paraguariensis |
title_full_unstemmed |
Callus induction in vitro on leaves explants of Ilex paraguariensis |
title_sort |
Callus induction in vitro on leaves explants of Ilex paraguariensis |
author |
Stachevski, Thamires Weigert |
author_facet |
Stachevski, Thamires Weigert Franciscon, Luziane Degenhardt-Goldbach, Juliana |
author_role |
author |
author2 |
Franciscon, Luziane Degenhardt-Goldbach, Juliana |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Stachevski, Thamires Weigert Franciscon, Luziane Degenhardt-Goldbach, Juliana |
dc.subject.por.fl_str_mv |
Zeatin 2 4-D Rizogenesis Organogenesis Zeatina 2 4-D Rizogênese Organogênese |
topic |
Zeatin 2 4-D Rizogenesis Organogenesis Zeatina 2 4-D Rizogênese Organogênese |
description |
Organogenesis is a technique rarely studied in the micro propagation of Ilex paraguariensis. This study evaluated different culture media on callus induction and in vitro organogenesis of this species. Leaves were collected from greenhouse grown plants. Leaves segments were placed on culture media ¼ MS, WPM or JADS, containing zeatin and 2,4-D. The MS medium was more efficient in callus inducing. Rooting was observed in the WPM medium. No buds were formed in any of the media evaluated. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/441 10.4336/2013.pfb.33.75.441 |
url |
https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/441 |
identifier_str_mv |
10.4336/2013.pfb.33.75.441 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/441/332 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Embrapa Florestas |
publisher.none.fl_str_mv |
Embrapa Florestas |
dc.source.none.fl_str_mv |
Pesquisa Florestal Brasileira; v. 33 n. 75 (2013): jul./set.; 339-342 Pesquisa Florestal Brasileira; Vol. 33 No. 75 (2013): jul./set.; 339-342 1983-2605 1809-3647 reponame:Pesquisa Florestal Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Florestal Brasileira (Online) |
collection |
Pesquisa Florestal Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Florestal Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pfb@embrapa.br || revista.pfb@gmail.com || patricia.mattos@embrapa.br |
_version_ |
1783370934149709824 |