Simultaneous analysis of sugars and organic acids in wine and grape juices by HPLC: Method validation and characterization of products from northeast Brazil.

Detalhes bibliográficos
Autor(a) principal: COELHO, E. M.
Data de Publicação: 2018
Outros Autores: PADILHA, C. V. da S., MISKINIS, G. A., SÁ, A. G. B. de, PEREIRA, G. E., AZEVEDO, L. C. de, LIMA, M. dos S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1089626
Resumo: Organic acids and sugars are related to the chemical balance of wines and grape juices, besides exerting a strong influence on the taste balance and sensorial acceptance by consumers. The aim of this study was to validate a method for the simultaneous determination of sugars and organic acids in wines and grape juices by highperformance liquid chromatography (HPLC) with refractive index detection (RID) and diode array detection (DAD) and to characterize commercial products from northeast Brazil. The method provided values for linearity (R > 0.9982), precision (CV% < 1.4), recovery (76–106%) and limits of detection (0.003–0.044 g L?1 ) and quantification (0.008–0.199 g L?1 ) which are considered acceptable for application in the characterization of these types of matrices. Principal components analysis (PCA) was used to verify the applicability of the method in the quality control of the products and resulted in the correct separation of the samples according to their type of processing. The results obtained in the characterization of the samples studied showed high levels of glucose and fructose in grape juice and the organic acids content was similar to those found in products originating from other regions around the world. Keywords: Food analysis, Food composition, Vitis labrusca L, Beverage analysis, Wine, Grape juice, Sparkling wine, Sugars, Organic acids
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spelling Simultaneous analysis of sugars and organic acids in wine and grape juices by HPLC: Method validation and characterization of products from northeast Brazil.Vitis labrusca LBeverage analysisOrganic AcidsSparkling wineWineFood analysisFood compositionGrape juiceSugarsOrganic acids and sugars are related to the chemical balance of wines and grape juices, besides exerting a strong influence on the taste balance and sensorial acceptance by consumers. The aim of this study was to validate a method for the simultaneous determination of sugars and organic acids in wines and grape juices by highperformance liquid chromatography (HPLC) with refractive index detection (RID) and diode array detection (DAD) and to characterize commercial products from northeast Brazil. The method provided values for linearity (R > 0.9982), precision (CV% < 1.4), recovery (76–106%) and limits of detection (0.003–0.044 g L?1 ) and quantification (0.008–0.199 g L?1 ) which are considered acceptable for application in the characterization of these types of matrices. Principal components analysis (PCA) was used to verify the applicability of the method in the quality control of the products and resulted in the correct separation of the samples according to their type of processing. The results obtained in the characterization of the samples studied showed high levels of glucose and fructose in grape juice and the organic acids content was similar to those found in products originating from other regions around the world. Keywords: Food analysis, Food composition, Vitis labrusca L, Beverage analysis, Wine, Grape juice, Sparkling wine, Sugars, Organic acidsEmanuela Monteiro Coelho, Instituto Federal do Sert ã o Pernambucano, Departamento de Tecnologia em Alimentos, Campus Petrolina, Rod. BR 407 Km 08, S/N, Jardim S ã o Paulo, CEP 56314-520, Petrolina, PE, Brazil; Carla Valéria da Silva Padilha, Instituto Federal do Sert ã o Pernambucano, Departamento de Tecnologia em Alimentos, Campus Petrolina, Rod. BR 407 Km 08, S/N, Jardim S ã o Paulo, CEP 56314-520, Petrolina, PE, Brazil; Gabriela Aquino Miskinis, Instituto Federal do Sert ã o Pernambucano, Departamento de Tecnologia em Alimentos, Campus Petrolina, Rod. BR 407 Km 08, S/N, Jardim S ã o Paulo, CEP 56314-520, Petrolina, PE, Brazil; Antônio Gomes Barroso de Sá, Instituto Federal do Sert ã o Pernambucano, Departamento de Tecnologia em Alimentos, Campus Petrolina, Rod. BR 407 Km 08, S/N, Jardim S ã o Paulo, CEP 56314-520, Petrolina, PE, Brazil; GIULIANO ELIAS PEREIRA, CNPUV; Luciana Cavalcanti de Azevêdo, Instituto Federal do Sert ã o Pernambucano, Departamento de Tecnologia em Alimentos, Campus Petrolina, Rod. BR 407 Km 08, S/N, Jardim S ã o Paulo, CEP 56314-520, Petrolina, PE, Brazil; Marcos dos Santos Lima, Instituto Federal do Sert ã o Pernambucano, Departamento de Tecnologia em Alimentos, Campus Petrolina, Rod. BR 407 Km 08, S/N, Jardim S ã o Paulo, CEP 56314-520, Petrolina, PE, Brazil.COELHO, E. M.PADILHA, C. V. da S.MISKINIS, G. A.SÁ, A. G. B. dePEREIRA, G. E.AZEVEDO, L. C. deLIMA, M. dos S.2018-03-23T01:03:02Z2018-03-23T01:03:02Z2018-03-2220182019-04-29T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleJournal of Food Composition and Analysis, v. 66, p. 160-167, 2018.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1089626enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-03-23T01:03:09Zoai:www.alice.cnptia.embrapa.br:doc/1089626Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542018-03-23T01:03:09falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-03-23T01:03:09Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Simultaneous analysis of sugars and organic acids in wine and grape juices by HPLC: Method validation and characterization of products from northeast Brazil.
title Simultaneous analysis of sugars and organic acids in wine and grape juices by HPLC: Method validation and characterization of products from northeast Brazil.
spellingShingle Simultaneous analysis of sugars and organic acids in wine and grape juices by HPLC: Method validation and characterization of products from northeast Brazil.
COELHO, E. M.
Vitis labrusca L
Beverage analysis
Organic Acids
Sparkling wine
Wine
Food analysis
Food composition
Grape juice
Sugars
title_short Simultaneous analysis of sugars and organic acids in wine and grape juices by HPLC: Method validation and characterization of products from northeast Brazil.
title_full Simultaneous analysis of sugars and organic acids in wine and grape juices by HPLC: Method validation and characterization of products from northeast Brazil.
title_fullStr Simultaneous analysis of sugars and organic acids in wine and grape juices by HPLC: Method validation and characterization of products from northeast Brazil.
title_full_unstemmed Simultaneous analysis of sugars and organic acids in wine and grape juices by HPLC: Method validation and characterization of products from northeast Brazil.
title_sort Simultaneous analysis of sugars and organic acids in wine and grape juices by HPLC: Method validation and characterization of products from northeast Brazil.
author COELHO, E. M.
author_facet COELHO, E. M.
PADILHA, C. V. da S.
MISKINIS, G. A.
SÁ, A. G. B. de
PEREIRA, G. E.
AZEVEDO, L. C. de
LIMA, M. dos S.
author_role author
author2 PADILHA, C. V. da S.
MISKINIS, G. A.
SÁ, A. G. B. de
PEREIRA, G. E.
AZEVEDO, L. C. de
LIMA, M. dos S.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Emanuela Monteiro Coelho, Instituto Federal do Sert ã o Pernambucano, Departamento de Tecnologia em Alimentos, Campus Petrolina, Rod. BR 407 Km 08, S/N, Jardim S ã o Paulo, CEP 56314-520, Petrolina, PE, Brazil; Carla Valéria da Silva Padilha, Instituto Federal do Sert ã o Pernambucano, Departamento de Tecnologia em Alimentos, Campus Petrolina, Rod. BR 407 Km 08, S/N, Jardim S ã o Paulo, CEP 56314-520, Petrolina, PE, Brazil; Gabriela Aquino Miskinis, Instituto Federal do Sert ã o Pernambucano, Departamento de Tecnologia em Alimentos, Campus Petrolina, Rod. BR 407 Km 08, S/N, Jardim S ã o Paulo, CEP 56314-520, Petrolina, PE, Brazil; Antônio Gomes Barroso de Sá, Instituto Federal do Sert ã o Pernambucano, Departamento de Tecnologia em Alimentos, Campus Petrolina, Rod. BR 407 Km 08, S/N, Jardim S ã o Paulo, CEP 56314-520, Petrolina, PE, Brazil; GIULIANO ELIAS PEREIRA, CNPUV; Luciana Cavalcanti de Azevêdo, Instituto Federal do Sert ã o Pernambucano, Departamento de Tecnologia em Alimentos, Campus Petrolina, Rod. BR 407 Km 08, S/N, Jardim S ã o Paulo, CEP 56314-520, Petrolina, PE, Brazil; Marcos dos Santos Lima, Instituto Federal do Sert ã o Pernambucano, Departamento de Tecnologia em Alimentos, Campus Petrolina, Rod. BR 407 Km 08, S/N, Jardim S ã o Paulo, CEP 56314-520, Petrolina, PE, Brazil.
dc.contributor.author.fl_str_mv COELHO, E. M.
PADILHA, C. V. da S.
MISKINIS, G. A.
SÁ, A. G. B. de
PEREIRA, G. E.
AZEVEDO, L. C. de
LIMA, M. dos S.
dc.subject.por.fl_str_mv Vitis labrusca L
Beverage analysis
Organic Acids
Sparkling wine
Wine
Food analysis
Food composition
Grape juice
Sugars
topic Vitis labrusca L
Beverage analysis
Organic Acids
Sparkling wine
Wine
Food analysis
Food composition
Grape juice
Sugars
description Organic acids and sugars are related to the chemical balance of wines and grape juices, besides exerting a strong influence on the taste balance and sensorial acceptance by consumers. The aim of this study was to validate a method for the simultaneous determination of sugars and organic acids in wines and grape juices by highperformance liquid chromatography (HPLC) with refractive index detection (RID) and diode array detection (DAD) and to characterize commercial products from northeast Brazil. The method provided values for linearity (R > 0.9982), precision (CV% < 1.4), recovery (76–106%) and limits of detection (0.003–0.044 g L?1 ) and quantification (0.008–0.199 g L?1 ) which are considered acceptable for application in the characterization of these types of matrices. Principal components analysis (PCA) was used to verify the applicability of the method in the quality control of the products and resulted in the correct separation of the samples according to their type of processing. The results obtained in the characterization of the samples studied showed high levels of glucose and fructose in grape juice and the organic acids content was similar to those found in products originating from other regions around the world. Keywords: Food analysis, Food composition, Vitis labrusca L, Beverage analysis, Wine, Grape juice, Sparkling wine, Sugars, Organic acids
publishDate 2018
dc.date.none.fl_str_mv 2018-03-23T01:03:02Z
2018-03-23T01:03:02Z
2018-03-22
2018
2019-04-29T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Journal of Food Composition and Analysis, v. 66, p. 160-167, 2018.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1089626
identifier_str_mv Journal of Food Composition and Analysis, v. 66, p. 160-167, 2018.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1089626
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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