Genome-wide association studies of anthracnose and angular leaf spot resistance in common bean (Phaseolus vulgaris L.).
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076304 |
Resumo: | The common bean (Phaseolus vulgaris L.) is the world?s most important legume for human consumption. Anthracnose (ANT; Colletotrichum lindemuthianum) and angular leaf spot (ALS; Pseudocercospora griseola) are complex diseases that cause major yield losses in common bean. Depending on the cultivar and environmental conditions, anthracnose and angular leaf spot infections can reduce crop yield drastically. This study aimed to estimate linkage disequilibrium levels and identify quantitative resistance loci (QRL) controlling resistance to both ANT and ALS diseases of 180 accessions of common bean using genomewide association analysis. A randomized complete block design with four replicates was performed for the ANT and ALS experiments, with four plants per genotype in each replicate. Association mapping analyses were performed for ANT and ALS using a mixed linear model approach implemented in TASSEL. A total of 17 and 11 significant statistically associations involving SSRs were detected for ANT and ALS resistance loci, respectively. Using SNPs, 21 and 17 significant statistically associations were obtained for ANT and angular ALS, respectively, providing more associations with this marker. The SSR-IAC167 and PvM95 markers, both located on chromosome Pv03, and the SNP scaffold00021_89379, were associated with both diseases. The other markers were distributed across the entire common bean genome, with chromosomes Pv03 and Pv08 showing the greatest number of loci associated with ANT resistance. The chromosome Pv04 was the most saturated one, with six markers associated with ALS resistance. The telomeric region of this chromosome showed four markers located between approximately 2.5 Mb and 4.4 Mb. Our results demonstrate the great potential of genome-wide association studies to identify QRLs related to ANT and ALS in common bean. The results indicate a quantitative and complex inheritance pattern for both diseases in common bean. Our findings will contribute to more effective screening of elite germplasm to find resistance alleles for marker-assisted selection in breeding programs. |
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Genome-wide association studies of anthracnose and angular leaf spot resistance in common bean (Phaseolus vulgaris L.).FeijãoPhaseolus vulgarisMancha foliarAntracnoseColletotrichum lindemuthianumGenomaThe common bean (Phaseolus vulgaris L.) is the world?s most important legume for human consumption. Anthracnose (ANT; Colletotrichum lindemuthianum) and angular leaf spot (ALS; Pseudocercospora griseola) are complex diseases that cause major yield losses in common bean. Depending on the cultivar and environmental conditions, anthracnose and angular leaf spot infections can reduce crop yield drastically. This study aimed to estimate linkage disequilibrium levels and identify quantitative resistance loci (QRL) controlling resistance to both ANT and ALS diseases of 180 accessions of common bean using genomewide association analysis. A randomized complete block design with four replicates was performed for the ANT and ALS experiments, with four plants per genotype in each replicate. Association mapping analyses were performed for ANT and ALS using a mixed linear model approach implemented in TASSEL. A total of 17 and 11 significant statistically associations involving SSRs were detected for ANT and ALS resistance loci, respectively. Using SNPs, 21 and 17 significant statistically associations were obtained for ANT and angular ALS, respectively, providing more associations with this marker. The SSR-IAC167 and PvM95 markers, both located on chromosome Pv03, and the SNP scaffold00021_89379, were associated with both diseases. The other markers were distributed across the entire common bean genome, with chromosomes Pv03 and Pv08 showing the greatest number of loci associated with ANT resistance. The chromosome Pv04 was the most saturated one, with six markers associated with ALS resistance. The telomeric region of this chromosome showed four markers located between approximately 2.5 Mb and 4.4 Mb. Our results demonstrate the great potential of genome-wide association studies to identify QRLs related to ANT and ALS in common bean. The results indicate a quantitative and complex inheritance pattern for both diseases in common bean. Our findings will contribute to more effective screening of elite germplasm to find resistance alleles for marker-assisted selection in breeding programs.JULIANA MORINI KÜPPER CARDOSO, IAC; PAULA RODRIGUES OBLESSUC, LABORATÓRIO NACIONAL DE BIOCIÊNCIAS, Campinas-SP; JOÃO RICARDO BACHEGA FEIJÓ ROSA, ESALQ; KLEBER ALVES GOMES, IAC; ALISSON FERNANDO CHIORATO, IAC; SERGIO AUGUSTO MORAIS CARBONELL, IAC; ANTONIO AUGUSTO FRANCO GARCIA, ESALQ; ROSANA PEREIRA VIANELLO, CNPAF; LUCIANA LASRY BENCHIMOL-REIS, IAC.PERSEGUINI, J. M. K. C.OBLESSUC, P. R.ROSA, J. R. B. F.GOMES, K. A.CHIORATO, A. F.CARBONELL, S. A. M.GARCIA, A. A. F.VIANELLO, R. P.BENCHIMOL-REIS, L. L.2017-09-27T10:34:08Z2017-09-27T10:34:08Z2017-09-2620162017-09-27T10:34:08Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlos One, v. 11, n. 3, e0150506, Mar. 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/107630410.1371/journal.pone.0150506enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-09-27T10:34:15Zoai:www.alice.cnptia.embrapa.br:doc/1076304Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-09-27T10:34:15falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-09-27T10:34:15Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Genome-wide association studies of anthracnose and angular leaf spot resistance in common bean (Phaseolus vulgaris L.). |
title |
Genome-wide association studies of anthracnose and angular leaf spot resistance in common bean (Phaseolus vulgaris L.). |
spellingShingle |
Genome-wide association studies of anthracnose and angular leaf spot resistance in common bean (Phaseolus vulgaris L.). PERSEGUINI, J. M. K. C. Feijão Phaseolus vulgaris Mancha foliar Antracnose Colletotrichum lindemuthianum Genoma |
title_short |
Genome-wide association studies of anthracnose and angular leaf spot resistance in common bean (Phaseolus vulgaris L.). |
title_full |
Genome-wide association studies of anthracnose and angular leaf spot resistance in common bean (Phaseolus vulgaris L.). |
title_fullStr |
Genome-wide association studies of anthracnose and angular leaf spot resistance in common bean (Phaseolus vulgaris L.). |
title_full_unstemmed |
Genome-wide association studies of anthracnose and angular leaf spot resistance in common bean (Phaseolus vulgaris L.). |
title_sort |
Genome-wide association studies of anthracnose and angular leaf spot resistance in common bean (Phaseolus vulgaris L.). |
author |
PERSEGUINI, J. M. K. C. |
author_facet |
PERSEGUINI, J. M. K. C. OBLESSUC, P. R. ROSA, J. R. B. F. GOMES, K. A. CHIORATO, A. F. CARBONELL, S. A. M. GARCIA, A. A. F. VIANELLO, R. P. BENCHIMOL-REIS, L. L. |
author_role |
author |
author2 |
OBLESSUC, P. R. ROSA, J. R. B. F. GOMES, K. A. CHIORATO, A. F. CARBONELL, S. A. M. GARCIA, A. A. F. VIANELLO, R. P. BENCHIMOL-REIS, L. L. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
JULIANA MORINI KÜPPER CARDOSO, IAC; PAULA RODRIGUES OBLESSUC, LABORATÓRIO NACIONAL DE BIOCIÊNCIAS, Campinas-SP; JOÃO RICARDO BACHEGA FEIJÓ ROSA, ESALQ; KLEBER ALVES GOMES, IAC; ALISSON FERNANDO CHIORATO, IAC; SERGIO AUGUSTO MORAIS CARBONELL, IAC; ANTONIO AUGUSTO FRANCO GARCIA, ESALQ; ROSANA PEREIRA VIANELLO, CNPAF; LUCIANA LASRY BENCHIMOL-REIS, IAC. |
dc.contributor.author.fl_str_mv |
PERSEGUINI, J. M. K. C. OBLESSUC, P. R. ROSA, J. R. B. F. GOMES, K. A. CHIORATO, A. F. CARBONELL, S. A. M. GARCIA, A. A. F. VIANELLO, R. P. BENCHIMOL-REIS, L. L. |
dc.subject.por.fl_str_mv |
Feijão Phaseolus vulgaris Mancha foliar Antracnose Colletotrichum lindemuthianum Genoma |
topic |
Feijão Phaseolus vulgaris Mancha foliar Antracnose Colletotrichum lindemuthianum Genoma |
description |
The common bean (Phaseolus vulgaris L.) is the world?s most important legume for human consumption. Anthracnose (ANT; Colletotrichum lindemuthianum) and angular leaf spot (ALS; Pseudocercospora griseola) are complex diseases that cause major yield losses in common bean. Depending on the cultivar and environmental conditions, anthracnose and angular leaf spot infections can reduce crop yield drastically. This study aimed to estimate linkage disequilibrium levels and identify quantitative resistance loci (QRL) controlling resistance to both ANT and ALS diseases of 180 accessions of common bean using genomewide association analysis. A randomized complete block design with four replicates was performed for the ANT and ALS experiments, with four plants per genotype in each replicate. Association mapping analyses were performed for ANT and ALS using a mixed linear model approach implemented in TASSEL. A total of 17 and 11 significant statistically associations involving SSRs were detected for ANT and ALS resistance loci, respectively. Using SNPs, 21 and 17 significant statistically associations were obtained for ANT and angular ALS, respectively, providing more associations with this marker. The SSR-IAC167 and PvM95 markers, both located on chromosome Pv03, and the SNP scaffold00021_89379, were associated with both diseases. The other markers were distributed across the entire common bean genome, with chromosomes Pv03 and Pv08 showing the greatest number of loci associated with ANT resistance. The chromosome Pv04 was the most saturated one, with six markers associated with ALS resistance. The telomeric region of this chromosome showed four markers located between approximately 2.5 Mb and 4.4 Mb. Our results demonstrate the great potential of genome-wide association studies to identify QRLs related to ANT and ALS in common bean. The results indicate a quantitative and complex inheritance pattern for both diseases in common bean. Our findings will contribute to more effective screening of elite germplasm to find resistance alleles for marker-assisted selection in breeding programs. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2017-09-27T10:34:08Z 2017-09-27T10:34:08Z 2017-09-26 2017-09-27T10:34:08Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Plos One, v. 11, n. 3, e0150506, Mar. 2016. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076304 10.1371/journal.pone.0150506 |
identifier_str_mv |
Plos One, v. 11, n. 3, e0150506, Mar. 2016. 10.1371/journal.pone.0150506 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076304 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503442623889408 |