Agrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants.

Detalhes bibliográficos
Autor(a) principal: MUNIZ, C. R.
Data de Publicação: 2014
Outros Autores: SILVA, G. F. da, SOUZA JUNIOR, M. T., FREIRE, F. das C. O., KEMA, G. H. J., GUEDES, M. I. F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/985915
Resumo: Lasiodiplodia theobromae is a major pathogen of many different crop cultures, including cashew nut plants. This paper describes an efficient Agrobacterium tumefaciens-mediated transformation (ATMT) system for the successful delivery of T-DNA, transferring the genes of green fluorescent protein (gfp) and hygromycin B phosphotransferase (hph) to L. theobromae. When the fungal pycnidiospores were co-cultured with A. tumefaciens harboring the binary vector with hph-gfp gene, hygromycin-resistant fungus only developed with acetosyringone supplementation.
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spelling Agrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants.Cashew plantsMediated transformationAgrobacterium TumefaciensLasiodiplodia TheobromaegummosisLasiodiplodia theobromae is a major pathogen of many different crop cultures, including cashew nut plants. This paper describes an efficient Agrobacterium tumefaciens-mediated transformation (ATMT) system for the successful delivery of T-DNA, transferring the genes of green fluorescent protein (gfp) and hygromycin B phosphotransferase (hph) to L. theobromae. When the fungal pycnidiospores were co-cultured with A. tumefaciens harboring the binary vector with hph-gfp gene, hygromycin-resistant fungus only developed with acetosyringone supplementation.CELLI RODRIGUES MUNIZ, CNPAT; GILVAN FERREIRA DA SILVA, CPAA; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE; FRANCISCO DAS CHAGAS O FREIRE, CNPAT; G. H. J. Kema, Biointeractions and Plant Health; M. I. F. Guedes, Universidade Estadual do Ceará.MUNIZ, C. R.SILVA, G. F. daSOUZA JUNIOR, M. T.FREIRE, F. das C. O.KEMA, G. H. J.GUEDES, M. I. F.2014-05-13T01:35:27Z2014-05-13T01:35:27Z2014-05-1220142014-05-13T01:35:27Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, v. 13, n. 2, p. 2906-2913, 2014.http://www.alice.cnptia.embrapa.br/alice/handle/doc/985915DOI http://dx.doi.org/10.4238/2014.February.21.8enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T00:16:50Zoai:www.alice.cnptia.embrapa.br:doc/985915Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T00:16:50Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Agrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants.
title Agrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants.
spellingShingle Agrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants.
MUNIZ, C. R.
Cashew plants
Mediated transformation
Agrobacterium Tumefaciens
Lasiodiplodia Theobromae
gummosis
title_short Agrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants.
title_full Agrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants.
title_fullStr Agrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants.
title_full_unstemmed Agrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants.
title_sort Agrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants.
author MUNIZ, C. R.
author_facet MUNIZ, C. R.
SILVA, G. F. da
SOUZA JUNIOR, M. T.
FREIRE, F. das C. O.
KEMA, G. H. J.
GUEDES, M. I. F.
author_role author
author2 SILVA, G. F. da
SOUZA JUNIOR, M. T.
FREIRE, F. das C. O.
KEMA, G. H. J.
GUEDES, M. I. F.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv CELLI RODRIGUES MUNIZ, CNPAT; GILVAN FERREIRA DA SILVA, CPAA; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE; FRANCISCO DAS CHAGAS O FREIRE, CNPAT; G. H. J. Kema, Biointeractions and Plant Health; M. I. F. Guedes, Universidade Estadual do Ceará.
dc.contributor.author.fl_str_mv MUNIZ, C. R.
SILVA, G. F. da
SOUZA JUNIOR, M. T.
FREIRE, F. das C. O.
KEMA, G. H. J.
GUEDES, M. I. F.
dc.subject.por.fl_str_mv Cashew plants
Mediated transformation
Agrobacterium Tumefaciens
Lasiodiplodia Theobromae
gummosis
topic Cashew plants
Mediated transformation
Agrobacterium Tumefaciens
Lasiodiplodia Theobromae
gummosis
description Lasiodiplodia theobromae is a major pathogen of many different crop cultures, including cashew nut plants. This paper describes an efficient Agrobacterium tumefaciens-mediated transformation (ATMT) system for the successful delivery of T-DNA, transferring the genes of green fluorescent protein (gfp) and hygromycin B phosphotransferase (hph) to L. theobromae. When the fungal pycnidiospores were co-cultured with A. tumefaciens harboring the binary vector with hph-gfp gene, hygromycin-resistant fungus only developed with acetosyringone supplementation.
publishDate 2014
dc.date.none.fl_str_mv 2014-05-13T01:35:27Z
2014-05-13T01:35:27Z
2014-05-12
2014
2014-05-13T01:35:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Genetics and Molecular Research, v. 13, n. 2, p. 2906-2913, 2014.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/985915
DOI http://dx.doi.org/10.4238/2014.February.21.8
identifier_str_mv Genetics and Molecular Research, v. 13, n. 2, p. 2906-2913, 2014.
DOI http://dx.doi.org/10.4238/2014.February.21.8
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/985915
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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