Enrichment and acclimation of an anaerobic mesophilic microorganism?s inoculum for standardization of BMP assays.

• Autor(a) principal: STEINMETZ, R. L. R.
• Data de Publicação: 2016
• Outros Autores: MEZZARI, M. P., SILVA, M. L. B. da, KUNZ, A., AMARAL, A. C., TÁPPARO, D. C., SOARES, H. M.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1058360; http://dx.doi.org/10.1016/j.biortech.2016.07.031
• topic: Digestão anaeróbica; Dejeto; Biogás; Anaerobic digestion; Waste treatment

Appropriate enrichment of anaerobic microorganism?s consortium is crucial for accurate biochemical methane potential (BMP) assays. An alternative method to produce and maintain a mesophilic methanogenic inoculum was demonstrated. Three sources of inoculum were mixed and acclimated for 857 days in order to reach steady conditions (pH = 7.90 ± 0.46; VS/TS > 50%; VFA/alkalinity = 0.16 ± 0.04 gAcetic Acid/gCaCO3 ). Biogas yield >80% was obtained after 70 days of inoculum acclimation in comparison to standard cellulose (>600 mLN/gVS). Methanogen community analysis based on 16S rDNA of the inoculum revealed Archaea concentration of 3 1012 gene copies/g (Methanobacteriales 8 1010; Methanomicrobiales 8 1010; and Methanosarcinales 4 1011 gene copies/g). The proposed method for development and maintenance of microorganism enrichment inoculum demonstrates consistent BMP data which is a requirement for dependable prediction of biogas production at field scale operations.