Serologies use in the identification of insects predators prey.
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1053654 |
Resumo: | This study aimed to produce antiserum for the main pests found in maize, Spodoptera frugiperda, Helicoverpa zea and armigera, Rhopalosiphum maidis, and uses it to determine their predators. Pest samples were macerated in 0.85% saline solution. The macerated were centrifuged and the supernatants were used as immunizing antigens to obtain the antiserum. For this purpose, a rabbit was immunized with 3.0 ml of the immunizing antigen on the lymph node region. Homologous serological tests were performed by double diffusion in agar. The homologous serological reactions were positive after seven days of antigen inoculation. The technique has sensitivity to detect predation of the pests studied. The tests were positive for a prey in the digestive tract of the predator to 96 hours of ingestion. Regarding the number of prey ingested there was no difference in the lines. It is concluded that after a single injection of antigen in rabbit lymph node region, it was possible to get an antiserum specific for pests of corn. Field tests showed that there was a certain food preference of Doru luteipes by S. frugiperda, Chrysoperla externa by aphid. |
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Serologies use in the identification of insects predators prey.PredaçãoZea maysAntígenoAntissoroAntigensAntiserumPredationThis study aimed to produce antiserum for the main pests found in maize, Spodoptera frugiperda, Helicoverpa zea and armigera, Rhopalosiphum maidis, and uses it to determine their predators. Pest samples were macerated in 0.85% saline solution. The macerated were centrifuged and the supernatants were used as immunizing antigens to obtain the antiserum. For this purpose, a rabbit was immunized with 3.0 ml of the immunizing antigen on the lymph node region. Homologous serological tests were performed by double diffusion in agar. The homologous serological reactions were positive after seven days of antigen inoculation. The technique has sensitivity to detect predation of the pests studied. The tests were positive for a prey in the digestive tract of the predator to 96 hours of ingestion. Regarding the number of prey ingested there was no difference in the lines. It is concluded that after a single injection of antigen in rabbit lymph node region, it was possible to get an antiserum specific for pests of corn. Field tests showed that there was a certain food preference of Doru luteipes by S. frugiperda, Chrysoperla externa by aphid.ANA CAROLINA MACIEL REDOAN, UFSCAR, São Carlos, SP.; CARLOS ROBERTO SOUSA-SILVA, UFSCAR, São Carlos, SP.; IVAN CRUZ, CNPMS.REDOAN, A. C. M.SOUSA-SILVA, C. R.CRUZ, I.2016-09-28T11:11:11Z2016-09-28T11:11:11Z2016-09-2820162017-02-14T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista Brasileira de Milho e Sorgo, Sete Lagoas, v. 15, n. 2, p. 157-170, 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/105365410.18512/1980-6477/rbms.v15n2p157-170enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T03:13:29Zoai:www.alice.cnptia.embrapa.br:doc/1053654Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T03:13:29falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T03:13:29Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Serologies use in the identification of insects predators prey. |
title |
Serologies use in the identification of insects predators prey. |
spellingShingle |
Serologies use in the identification of insects predators prey. REDOAN, A. C. M. Predação Zea mays Antígeno Antissoro Antigens Antiserum Predation |
title_short |
Serologies use in the identification of insects predators prey. |
title_full |
Serologies use in the identification of insects predators prey. |
title_fullStr |
Serologies use in the identification of insects predators prey. |
title_full_unstemmed |
Serologies use in the identification of insects predators prey. |
title_sort |
Serologies use in the identification of insects predators prey. |
author |
REDOAN, A. C. M. |
author_facet |
REDOAN, A. C. M. SOUSA-SILVA, C. R. CRUZ, I. |
author_role |
author |
author2 |
SOUSA-SILVA, C. R. CRUZ, I. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
ANA CAROLINA MACIEL REDOAN, UFSCAR, São Carlos, SP.; CARLOS ROBERTO SOUSA-SILVA, UFSCAR, São Carlos, SP.; IVAN CRUZ, CNPMS. |
dc.contributor.author.fl_str_mv |
REDOAN, A. C. M. SOUSA-SILVA, C. R. CRUZ, I. |
dc.subject.por.fl_str_mv |
Predação Zea mays Antígeno Antissoro Antigens Antiserum Predation |
topic |
Predação Zea mays Antígeno Antissoro Antigens Antiserum Predation |
description |
This study aimed to produce antiserum for the main pests found in maize, Spodoptera frugiperda, Helicoverpa zea and armigera, Rhopalosiphum maidis, and uses it to determine their predators. Pest samples were macerated in 0.85% saline solution. The macerated were centrifuged and the supernatants were used as immunizing antigens to obtain the antiserum. For this purpose, a rabbit was immunized with 3.0 ml of the immunizing antigen on the lymph node region. Homologous serological tests were performed by double diffusion in agar. The homologous serological reactions were positive after seven days of antigen inoculation. The technique has sensitivity to detect predation of the pests studied. The tests were positive for a prey in the digestive tract of the predator to 96 hours of ingestion. Regarding the number of prey ingested there was no difference in the lines. It is concluded that after a single injection of antigen in rabbit lymph node region, it was possible to get an antiserum specific for pests of corn. Field tests showed that there was a certain food preference of Doru luteipes by S. frugiperda, Chrysoperla externa by aphid. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-28T11:11:11Z 2016-09-28T11:11:11Z 2016-09-28 2016 2017-02-14T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 15, n. 2, p. 157-170, 2016. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1053654 10.18512/1980-6477/rbms.v15n2p157-170 |
identifier_str_mv |
Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 15, n. 2, p. 157-170, 2016. 10.18512/1980-6477/rbms.v15n2p157-170 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1053654 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503426019688448 |