Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo.
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1036144 |
Resumo: | This study aimed to evaluate the efficacy of adding sucrose in vitrification solution of ovine embryos produced in vivo. Forty Dorper ewes were selected and superovulated. Immediately prior to the embryo collection by laparotomy, a laparoscopy was erformed to verify the superovulatory response. The recovered flushing was followed by embryo evaluation and embryos were divided in two experimental groups where embryos from Control group were submitted to a traditional vitrification protocol and embryos from ucrose group to a modified vitrification protocol with sucrose. After warming, embryos were again divided regarding cryoprotectant removal (Indirect) or not (Direct). The embryo quality was classified as embryos of degrees I (excellent or good), II (regular), III (poor) and IV (dead or degenerate). It was also verified the homogeneity of mass, occurrence of embryonic mass retraction and rupture of pellucid zone. The results were expressed as percentages and were subjected to Chi-square test with P < 0.05. The embryos vitrified in the presence of sucrose had lower proportions of lower-quality embryos after warming (22.20 vs. 44.50%), higher percentages of homogeneous embryos after warming (63.89 vs. 38.89 %) while concerning other parameters there was no difference between these groups. It can be concluded that the addition of 0.4 M sucrose during vitrification improves the embryo quality. |
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Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo.Raça DorperProdução animalOvinoCana de AçúcarCriopreservaçãoOvulaçãoAnimal productionSucroseThis study aimed to evaluate the efficacy of adding sucrose in vitrification solution of ovine embryos produced in vivo. Forty Dorper ewes were selected and superovulated. Immediately prior to the embryo collection by laparotomy, a laparoscopy was erformed to verify the superovulatory response. The recovered flushing was followed by embryo evaluation and embryos were divided in two experimental groups where embryos from Control group were submitted to a traditional vitrification protocol and embryos from ucrose group to a modified vitrification protocol with sucrose. After warming, embryos were again divided regarding cryoprotectant removal (Indirect) or not (Direct). The embryo quality was classified as embryos of degrees I (excellent or good), II (regular), III (poor) and IV (dead or degenerate). It was also verified the homogeneity of mass, occurrence of embryonic mass retraction and rupture of pellucid zone. The results were expressed as percentages and were subjected to Chi-square test with P < 0.05. The embryos vitrified in the presence of sucrose had lower proportions of lower-quality embryos after warming (22.20 vs. 44.50%), higher percentages of homogeneous embryos after warming (63.89 vs. 38.89 %) while concerning other parameters there was no difference between these groups. It can be concluded that the addition of 0.4 M sucrose during vitrification improves the embryo quality.Suplemento 2.ALANE PAINS OLIVEIRA DO MONTE, Mestranda da UNIVASF; JOÃO BOSCO LOIOLA FILHO, Mestrando da UNIVASF; THAIS THATIANE DOS SANTOS SOUZA, Mestranda da UNIVASF; MAYARA DE SOUZA MIRANDA, Mestranda da UNIVASF; LÍVIA CORREIA MAGALHÃES, Mestranda da UNIVASF; CELSO HENRIQUE SOUZA COSTA BARROS, Estudante da UNIVASF; ANA ARLETE DE AMORIM SILVA, Estudante da UNIVASF; ADRIANO OLIVEIRA SANTOS, Estudante da UNIVASF; AIONNE DE SOUZA LEITE GUIMARÃES, Estudante da UNIVASF; JONATHAN MAIA DA SILVA COSTA, Estudante da UNIVASF; PEDRO HUMBERTO FÉLIX DE SOUSA, Professor da Universidade do Estado da Bahia, UNEB, Juazeiro, BA; DANIEL MAIA NOGUEIRA, CPATSA; MABEL FREITAS CORDEIRO, Professora da UNIVASF; EDILSON SOARES LOPES JÚNIOR, Professor da UNIVASF.MONTE, A. P. O. doLOIOLA FILHO, J. B.SOUZA, T. T. dos S.MIRANDA, M. de S.MAGALHÃES, L. C.BARROS, C. H. S. C.SILVA, A. A. de A.SANTOS, A. O.GUIMARÃES, A. de S. L.COSTA, J. M. da S.SOUSA, P. H. F. deNOGUEIRA, D. M.CORDEIRO, M. F.LOPES JÚNIOR, E. S.2016-02-04T11:11:11Z2016-02-04T11:11:11Z2016-02-0420152016-03-29T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSemina: Ciências Agrárias, Londrina, v. 36, n. 6, p. 4257-4268, 2015.http://www.alice.cnptia.embrapa.br/alice/handle/doc/103614410.5433/1679-0359.2015v36n6Supl2p4257enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T03:40:07Zoai:www.alice.cnptia.embrapa.br:doc/1036144Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T03:40:07falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T03:40:07Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo. |
title |
Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo. |
spellingShingle |
Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo. MONTE, A. P. O. do Raça Dorper Produção animal Ovino Cana de Açúcar Criopreservação Ovulação Animal production Sucrose |
title_short |
Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo. |
title_full |
Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo. |
title_fullStr |
Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo. |
title_full_unstemmed |
Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo. |
title_sort |
Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo. |
author |
MONTE, A. P. O. do |
author_facet |
MONTE, A. P. O. do LOIOLA FILHO, J. B. SOUZA, T. T. dos S. MIRANDA, M. de S. MAGALHÃES, L. C. BARROS, C. H. S. C. SILVA, A. A. de A. SANTOS, A. O. GUIMARÃES, A. de S. L. COSTA, J. M. da S. SOUSA, P. H. F. de NOGUEIRA, D. M. CORDEIRO, M. F. LOPES JÚNIOR, E. S. |
author_role |
author |
author2 |
LOIOLA FILHO, J. B. SOUZA, T. T. dos S. MIRANDA, M. de S. MAGALHÃES, L. C. BARROS, C. H. S. C. SILVA, A. A. de A. SANTOS, A. O. GUIMARÃES, A. de S. L. COSTA, J. M. da S. SOUSA, P. H. F. de NOGUEIRA, D. M. CORDEIRO, M. F. LOPES JÚNIOR, E. S. |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
ALANE PAINS OLIVEIRA DO MONTE, Mestranda da UNIVASF; JOÃO BOSCO LOIOLA FILHO, Mestrando da UNIVASF; THAIS THATIANE DOS SANTOS SOUZA, Mestranda da UNIVASF; MAYARA DE SOUZA MIRANDA, Mestranda da UNIVASF; LÍVIA CORREIA MAGALHÃES, Mestranda da UNIVASF; CELSO HENRIQUE SOUZA COSTA BARROS, Estudante da UNIVASF; ANA ARLETE DE AMORIM SILVA, Estudante da UNIVASF; ADRIANO OLIVEIRA SANTOS, Estudante da UNIVASF; AIONNE DE SOUZA LEITE GUIMARÃES, Estudante da UNIVASF; JONATHAN MAIA DA SILVA COSTA, Estudante da UNIVASF; PEDRO HUMBERTO FÉLIX DE SOUSA, Professor da Universidade do Estado da Bahia, UNEB, Juazeiro, BA; DANIEL MAIA NOGUEIRA, CPATSA; MABEL FREITAS CORDEIRO, Professora da UNIVASF; EDILSON SOARES LOPES JÚNIOR, Professor da UNIVASF. |
dc.contributor.author.fl_str_mv |
MONTE, A. P. O. do LOIOLA FILHO, J. B. SOUZA, T. T. dos S. MIRANDA, M. de S. MAGALHÃES, L. C. BARROS, C. H. S. C. SILVA, A. A. de A. SANTOS, A. O. GUIMARÃES, A. de S. L. COSTA, J. M. da S. SOUSA, P. H. F. de NOGUEIRA, D. M. CORDEIRO, M. F. LOPES JÚNIOR, E. S. |
dc.subject.por.fl_str_mv |
Raça Dorper Produção animal Ovino Cana de Açúcar Criopreservação Ovulação Animal production Sucrose |
topic |
Raça Dorper Produção animal Ovino Cana de Açúcar Criopreservação Ovulação Animal production Sucrose |
description |
This study aimed to evaluate the efficacy of adding sucrose in vitrification solution of ovine embryos produced in vivo. Forty Dorper ewes were selected and superovulated. Immediately prior to the embryo collection by laparotomy, a laparoscopy was erformed to verify the superovulatory response. The recovered flushing was followed by embryo evaluation and embryos were divided in two experimental groups where embryos from Control group were submitted to a traditional vitrification protocol and embryos from ucrose group to a modified vitrification protocol with sucrose. After warming, embryos were again divided regarding cryoprotectant removal (Indirect) or not (Direct). The embryo quality was classified as embryos of degrees I (excellent or good), II (regular), III (poor) and IV (dead or degenerate). It was also verified the homogeneity of mass, occurrence of embryonic mass retraction and rupture of pellucid zone. The results were expressed as percentages and were subjected to Chi-square test with P < 0.05. The embryos vitrified in the presence of sucrose had lower proportions of lower-quality embryos after warming (22.20 vs. 44.50%), higher percentages of homogeneous embryos after warming (63.89 vs. 38.89 %) while concerning other parameters there was no difference between these groups. It can be concluded that the addition of 0.4 M sucrose during vitrification improves the embryo quality. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2016-02-04T11:11:11Z 2016-02-04T11:11:11Z 2016-02-04 2016-03-29T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Semina: Ciências Agrárias, Londrina, v. 36, n. 6, p. 4257-4268, 2015. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1036144 10.5433/1679-0359.2015v36n6Supl2p4257 |
identifier_str_mv |
Semina: Ciências Agrárias, Londrina, v. 36, n. 6, p. 4257-4268, 2015. 10.5433/1679-0359.2015v36n6Supl2p4257 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1036144 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503417559777280 |