Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo.

Detalhes bibliográficos
Autor(a) principal: MONTE, A. P. O. do
Data de Publicação: 2015
Outros Autores: LOIOLA FILHO, J. B., SOUZA, T. T. dos S., MIRANDA, M. de S., MAGALHÃES, L. C., BARROS, C. H. S. C., SILVA, A. A. de A., SANTOS, A. O., GUIMARÃES, A. de S. L., COSTA, J. M. da S., SOUSA, P. H. F. de, NOGUEIRA, D. M., CORDEIRO, M. F., LOPES JÚNIOR, E. S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1036144
Resumo: This study aimed to evaluate the efficacy of adding sucrose in vitrification solution of ovine embryos produced in vivo. Forty Dorper ewes were selected and superovulated. Immediately prior to the embryo collection by laparotomy, a laparoscopy was erformed to verify the superovulatory response. The recovered flushing was followed by embryo evaluation and embryos were divided in two experimental groups where embryos from Control group were submitted to a traditional vitrification protocol and embryos from ucrose group to a modified vitrification protocol with sucrose. After warming, embryos were again divided regarding cryoprotectant removal (Indirect) or not (Direct). The embryo quality was classified as embryos of degrees I (excellent or good), II (regular), III (poor) and IV (dead or degenerate). It was also verified the homogeneity of mass, occurrence of embryonic mass retraction and rupture of pellucid zone. The results were expressed as percentages and were subjected to Chi-square test with P < 0.05. The embryos vitrified in the presence of sucrose had lower proportions of lower-quality embryos after warming (22.20 vs. 44.50%), higher percentages of homogeneous embryos after warming (63.89 vs. 38.89 %) while concerning other parameters there was no difference between these groups. It can be concluded that the addition of 0.4 M sucrose during vitrification improves the embryo quality.
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spelling Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo.Raça DorperProdução animalOvinoCana de AçúcarCriopreservaçãoOvulaçãoAnimal productionSucroseThis study aimed to evaluate the efficacy of adding sucrose in vitrification solution of ovine embryos produced in vivo. Forty Dorper ewes were selected and superovulated. Immediately prior to the embryo collection by laparotomy, a laparoscopy was erformed to verify the superovulatory response. The recovered flushing was followed by embryo evaluation and embryos were divided in two experimental groups where embryos from Control group were submitted to a traditional vitrification protocol and embryos from ucrose group to a modified vitrification protocol with sucrose. After warming, embryos were again divided regarding cryoprotectant removal (Indirect) or not (Direct). The embryo quality was classified as embryos of degrees I (excellent or good), II (regular), III (poor) and IV (dead or degenerate). It was also verified the homogeneity of mass, occurrence of embryonic mass retraction and rupture of pellucid zone. The results were expressed as percentages and were subjected to Chi-square test with P < 0.05. The embryos vitrified in the presence of sucrose had lower proportions of lower-quality embryos after warming (22.20 vs. 44.50%), higher percentages of homogeneous embryos after warming (63.89 vs. 38.89 %) while concerning other parameters there was no difference between these groups. It can be concluded that the addition of 0.4 M sucrose during vitrification improves the embryo quality.Suplemento 2.ALANE PAINS OLIVEIRA DO MONTE, Mestranda da UNIVASF; JOÃO BOSCO LOIOLA FILHO, Mestrando da UNIVASF; THAIS THATIANE DOS SANTOS SOUZA, Mestranda da UNIVASF; MAYARA DE SOUZA MIRANDA, Mestranda da UNIVASF; LÍVIA CORREIA MAGALHÃES, Mestranda da UNIVASF; CELSO HENRIQUE SOUZA COSTA BARROS, Estudante da UNIVASF; ANA ARLETE DE AMORIM SILVA, Estudante da UNIVASF; ADRIANO OLIVEIRA SANTOS, Estudante da UNIVASF; AIONNE DE SOUZA LEITE GUIMARÃES, Estudante da UNIVASF; JONATHAN MAIA DA SILVA COSTA, Estudante da UNIVASF; PEDRO HUMBERTO FÉLIX DE SOUSA, Professor da Universidade do Estado da Bahia, UNEB, Juazeiro, BA; DANIEL MAIA NOGUEIRA, CPATSA; MABEL FREITAS CORDEIRO, Professora da UNIVASF; EDILSON SOARES LOPES JÚNIOR, Professor da UNIVASF.MONTE, A. P. O. doLOIOLA FILHO, J. B.SOUZA, T. T. dos S.MIRANDA, M. de S.MAGALHÃES, L. C.BARROS, C. H. S. C.SILVA, A. A. de A.SANTOS, A. O.GUIMARÃES, A. de S. L.COSTA, J. M. da S.SOUSA, P. H. F. deNOGUEIRA, D. M.CORDEIRO, M. F.LOPES JÚNIOR, E. S.2016-02-04T11:11:11Z2016-02-04T11:11:11Z2016-02-0420152016-03-29T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSemina: Ciências Agrárias, Londrina, v. 36, n. 6, p. 4257-4268, 2015.http://www.alice.cnptia.embrapa.br/alice/handle/doc/103614410.5433/1679-0359.2015v36n6Supl2p4257enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T03:40:07Zoai:www.alice.cnptia.embrapa.br:doc/1036144Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T03:40:07falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T03:40:07Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo.
title Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo.
spellingShingle Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo.
MONTE, A. P. O. do
Raça Dorper
Produção animal
Ovino
Cana de Açúcar
Criopreservação
Ovulação
Animal production
Sucrose
title_short Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo.
title_full Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo.
title_fullStr Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo.
title_full_unstemmed Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo.
title_sort Addition of sucrose on cryoprotectant solution of vitrification enhances the quality of Dorper sheep embryos produced in vivo.
author MONTE, A. P. O. do
author_facet MONTE, A. P. O. do
LOIOLA FILHO, J. B.
SOUZA, T. T. dos S.
MIRANDA, M. de S.
MAGALHÃES, L. C.
BARROS, C. H. S. C.
SILVA, A. A. de A.
SANTOS, A. O.
GUIMARÃES, A. de S. L.
COSTA, J. M. da S.
SOUSA, P. H. F. de
NOGUEIRA, D. M.
CORDEIRO, M. F.
LOPES JÚNIOR, E. S.
author_role author
author2 LOIOLA FILHO, J. B.
SOUZA, T. T. dos S.
MIRANDA, M. de S.
MAGALHÃES, L. C.
BARROS, C. H. S. C.
SILVA, A. A. de A.
SANTOS, A. O.
GUIMARÃES, A. de S. L.
COSTA, J. M. da S.
SOUSA, P. H. F. de
NOGUEIRA, D. M.
CORDEIRO, M. F.
LOPES JÚNIOR, E. S.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv ALANE PAINS OLIVEIRA DO MONTE, Mestranda da UNIVASF; JOÃO BOSCO LOIOLA FILHO, Mestrando da UNIVASF; THAIS THATIANE DOS SANTOS SOUZA, Mestranda da UNIVASF; MAYARA DE SOUZA MIRANDA, Mestranda da UNIVASF; LÍVIA CORREIA MAGALHÃES, Mestranda da UNIVASF; CELSO HENRIQUE SOUZA COSTA BARROS, Estudante da UNIVASF; ANA ARLETE DE AMORIM SILVA, Estudante da UNIVASF; ADRIANO OLIVEIRA SANTOS, Estudante da UNIVASF; AIONNE DE SOUZA LEITE GUIMARÃES, Estudante da UNIVASF; JONATHAN MAIA DA SILVA COSTA, Estudante da UNIVASF; PEDRO HUMBERTO FÉLIX DE SOUSA, Professor da Universidade do Estado da Bahia, UNEB, Juazeiro, BA; DANIEL MAIA NOGUEIRA, CPATSA; MABEL FREITAS CORDEIRO, Professora da UNIVASF; EDILSON SOARES LOPES JÚNIOR, Professor da UNIVASF.
dc.contributor.author.fl_str_mv MONTE, A. P. O. do
LOIOLA FILHO, J. B.
SOUZA, T. T. dos S.
MIRANDA, M. de S.
MAGALHÃES, L. C.
BARROS, C. H. S. C.
SILVA, A. A. de A.
SANTOS, A. O.
GUIMARÃES, A. de S. L.
COSTA, J. M. da S.
SOUSA, P. H. F. de
NOGUEIRA, D. M.
CORDEIRO, M. F.
LOPES JÚNIOR, E. S.
dc.subject.por.fl_str_mv Raça Dorper
Produção animal
Ovino
Cana de Açúcar
Criopreservação
Ovulação
Animal production
Sucrose
topic Raça Dorper
Produção animal
Ovino
Cana de Açúcar
Criopreservação
Ovulação
Animal production
Sucrose
description This study aimed to evaluate the efficacy of adding sucrose in vitrification solution of ovine embryos produced in vivo. Forty Dorper ewes were selected and superovulated. Immediately prior to the embryo collection by laparotomy, a laparoscopy was erformed to verify the superovulatory response. The recovered flushing was followed by embryo evaluation and embryos were divided in two experimental groups where embryos from Control group were submitted to a traditional vitrification protocol and embryos from ucrose group to a modified vitrification protocol with sucrose. After warming, embryos were again divided regarding cryoprotectant removal (Indirect) or not (Direct). The embryo quality was classified as embryos of degrees I (excellent or good), II (regular), III (poor) and IV (dead or degenerate). It was also verified the homogeneity of mass, occurrence of embryonic mass retraction and rupture of pellucid zone. The results were expressed as percentages and were subjected to Chi-square test with P < 0.05. The embryos vitrified in the presence of sucrose had lower proportions of lower-quality embryos after warming (22.20 vs. 44.50%), higher percentages of homogeneous embryos after warming (63.89 vs. 38.89 %) while concerning other parameters there was no difference between these groups. It can be concluded that the addition of 0.4 M sucrose during vitrification improves the embryo quality.
publishDate 2015
dc.date.none.fl_str_mv 2015
2016-02-04T11:11:11Z
2016-02-04T11:11:11Z
2016-02-04
2016-03-29T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Semina: Ciências Agrárias, Londrina, v. 36, n. 6, p. 4257-4268, 2015.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1036144
10.5433/1679-0359.2015v36n6Supl2p4257
identifier_str_mv Semina: Ciências Agrárias, Londrina, v. 36, n. 6, p. 4257-4268, 2015.
10.5433/1679-0359.2015v36n6Supl2p4257
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1036144
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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