Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis?
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159710 https://doi.org/10.1007/s42770-023-01181-1 |
Resumo: | Staphylococcus aureus is one of the agents of bovine mastitis of hardest control due to a complex pathogenesis comprising a variety of virulence factors, which ensures its persistence in the mammary gland, causing significant health and economic losses. Therefore, understanding the pathogenesis of this agent is imperative. Galleria mellonella has stood out as an invertebrate animal model for the study of infectious diseases that affect several hosts. This work aimed to evaluate G. mellonella larvae as an experimental model for the study of virulence phenotypes in an S. aureus population isolated from bovine mastitis. Thirty genetically divergent S. aureus strains were chosen based on PFGE analysis. After experimental infection, larvae survival rates, bacterial growth in hemolymph, melanization intensity of the dorsal vessel, and histological characteristics of the infected tissues were evaluated. The G. mellonella model showed a clear diversity in the S. aureus pathogenicity pattern, allowing the differentiation of strains with virulence phenotypes ranging from high to low degrees. Histological analysis confirmed that the strains tested were capable of inducing the formation of nodules and melanization spots in the dorsal vessels of the larvae in different magnitudes. The strains 16S-717, 19C-828, and 31S-1443 presented the highest virulence intensity among the bacteria tested and will be used further for the generation of S. aureus mutant populations to prospect genetic targets aimed to develop control strategies of bovine mastitis. Altogether, our results suggest that G. mellonella is an attractive and low-cost animal model for characterizing virulence phenotypes of large S. aureus populations. |
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Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis?Infecção intramamáriaVirulência bacterianaModelo de invertebradosBovinoDoença AnimalStaphylococcus aureus is one of the agents of bovine mastitis of hardest control due to a complex pathogenesis comprising a variety of virulence factors, which ensures its persistence in the mammary gland, causing significant health and economic losses. Therefore, understanding the pathogenesis of this agent is imperative. Galleria mellonella has stood out as an invertebrate animal model for the study of infectious diseases that affect several hosts. This work aimed to evaluate G. mellonella larvae as an experimental model for the study of virulence phenotypes in an S. aureus population isolated from bovine mastitis. Thirty genetically divergent S. aureus strains were chosen based on PFGE analysis. After experimental infection, larvae survival rates, bacterial growth in hemolymph, melanization intensity of the dorsal vessel, and histological characteristics of the infected tissues were evaluated. The G. mellonella model showed a clear diversity in the S. aureus pathogenicity pattern, allowing the differentiation of strains with virulence phenotypes ranging from high to low degrees. Histological analysis confirmed that the strains tested were capable of inducing the formation of nodules and melanization spots in the dorsal vessels of the larvae in different magnitudes. The strains 16S-717, 19C-828, and 31S-1443 presented the highest virulence intensity among the bacteria tested and will be used further for the generation of S. aureus mutant populations to prospect genetic targets aimed to develop control strategies of bovine mastitis. Altogether, our results suggest that G. mellonella is an attractive and low-cost animal model for characterizing virulence phenotypes of large S. aureus populations.JULIANA ROSA DA SILVA, UNIVERSIDADE FEDERAL DE LAVRAS; JOICE FÁTIMA MOREIRA SILVA, UNIVERSIDADE FEDERAL DE LAVRAS; MONALESSA FÁBIA PEREIRA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; ADALGISA RIBEIRO TORRES; MAYSA SERPA GONÇALVES, UNIVERSIDADE FEDERAL DE LAVRAS; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; MARIA APARECIDA VASCONCELOS PAIVA E BRITO; GERALDO MÁRCIO DA COSTA, UNIVERSIDADE FEDERAL DE LAVRAS; JOAO BATISTA RIBEIRO, CNPGL.SILVA, J. R. daSILVA, J. F. M.PEREIRA, M. F.TORRES, A. R.GONÇALVES, M. S.PRATA, M. C. de A.BRITO, M. A. V. P. eCOSTA, G. M. daRIBEIRO, J. B.2024-03-11T12:32:31Z2024-03-11T12:32:31Z2023-12-142024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrazilian Journal of Microbiology, v. 55, n. 1, p. 889-900, 2024.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159710https://doi.org/10.1007/s42770-023-01181-1enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2024-03-11T12:32:31Zoai:www.alice.cnptia.embrapa.br:doc/1159710Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542024-03-11T12:32:31falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542024-03-11T12:32:31Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis? |
title |
Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis? |
spellingShingle |
Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis? SILVA, J. R. da Infecção intramamária Virulência bacteriana Modelo de invertebrados Bovino Doença Animal |
title_short |
Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis? |
title_full |
Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis? |
title_fullStr |
Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis? |
title_full_unstemmed |
Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis? |
title_sort |
Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis? |
author |
SILVA, J. R. da |
author_facet |
SILVA, J. R. da SILVA, J. F. M. PEREIRA, M. F. TORRES, A. R. GONÇALVES, M. S. PRATA, M. C. de A. BRITO, M. A. V. P. e COSTA, G. M. da RIBEIRO, J. B. |
author_role |
author |
author2 |
SILVA, J. F. M. PEREIRA, M. F. TORRES, A. R. GONÇALVES, M. S. PRATA, M. C. de A. BRITO, M. A. V. P. e COSTA, G. M. da RIBEIRO, J. B. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
JULIANA ROSA DA SILVA, UNIVERSIDADE FEDERAL DE LAVRAS; JOICE FÁTIMA MOREIRA SILVA, UNIVERSIDADE FEDERAL DE LAVRAS; MONALESSA FÁBIA PEREIRA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; ADALGISA RIBEIRO TORRES; MAYSA SERPA GONÇALVES, UNIVERSIDADE FEDERAL DE LAVRAS; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; MARIA APARECIDA VASCONCELOS PAIVA E BRITO; GERALDO MÁRCIO DA COSTA, UNIVERSIDADE FEDERAL DE LAVRAS; JOAO BATISTA RIBEIRO, CNPGL. |
dc.contributor.author.fl_str_mv |
SILVA, J. R. da SILVA, J. F. M. PEREIRA, M. F. TORRES, A. R. GONÇALVES, M. S. PRATA, M. C. de A. BRITO, M. A. V. P. e COSTA, G. M. da RIBEIRO, J. B. |
dc.subject.por.fl_str_mv |
Infecção intramamária Virulência bacteriana Modelo de invertebrados Bovino Doença Animal |
topic |
Infecção intramamária Virulência bacteriana Modelo de invertebrados Bovino Doença Animal |
description |
Staphylococcus aureus is one of the agents of bovine mastitis of hardest control due to a complex pathogenesis comprising a variety of virulence factors, which ensures its persistence in the mammary gland, causing significant health and economic losses. Therefore, understanding the pathogenesis of this agent is imperative. Galleria mellonella has stood out as an invertebrate animal model for the study of infectious diseases that affect several hosts. This work aimed to evaluate G. mellonella larvae as an experimental model for the study of virulence phenotypes in an S. aureus population isolated from bovine mastitis. Thirty genetically divergent S. aureus strains were chosen based on PFGE analysis. After experimental infection, larvae survival rates, bacterial growth in hemolymph, melanization intensity of the dorsal vessel, and histological characteristics of the infected tissues were evaluated. The G. mellonella model showed a clear diversity in the S. aureus pathogenicity pattern, allowing the differentiation of strains with virulence phenotypes ranging from high to low degrees. Histological analysis confirmed that the strains tested were capable of inducing the formation of nodules and melanization spots in the dorsal vessels of the larvae in different magnitudes. The strains 16S-717, 19C-828, and 31S-1443 presented the highest virulence intensity among the bacteria tested and will be used further for the generation of S. aureus mutant populations to prospect genetic targets aimed to develop control strategies of bovine mastitis. Altogether, our results suggest that G. mellonella is an attractive and low-cost animal model for characterizing virulence phenotypes of large S. aureus populations. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-12-14 2024-03-11T12:32:31Z 2024-03-11T12:32:31Z 2024 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Brazilian Journal of Microbiology, v. 55, n. 1, p. 889-900, 2024. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159710 https://doi.org/10.1007/s42770-023-01181-1 |
identifier_str_mv |
Brazilian Journal of Microbiology, v. 55, n. 1, p. 889-900, 2024. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159710 https://doi.org/10.1007/s42770-023-01181-1 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503558956056576 |