Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis?

Detalhes bibliográficos
Autor(a) principal: SILVA, J. R. da
Data de Publicação: 2023
Outros Autores: SILVA, J. F. M., PEREIRA, M. F., TORRES, A. R., GONÇALVES, M. S., PRATA, M. C. de A., BRITO, M. A. V. P. e, COSTA, G. M. da, RIBEIRO, J. B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159710
https://doi.org/10.1007/s42770-023-01181-1
Resumo: Staphylococcus aureus is one of the agents of bovine mastitis of hardest control due to a complex pathogenesis comprising a variety of virulence factors, which ensures its persistence in the mammary gland, causing significant health and economic losses. Therefore, understanding the pathogenesis of this agent is imperative. Galleria mellonella has stood out as an invertebrate animal model for the study of infectious diseases that affect several hosts. This work aimed to evaluate G. mellonella larvae as an experimental model for the study of virulence phenotypes in an S. aureus population isolated from bovine mastitis. Thirty genetically divergent S. aureus strains were chosen based on PFGE analysis. After experimental infection, larvae survival rates, bacterial growth in hemolymph, melanization intensity of the dorsal vessel, and histological characteristics of the infected tissues were evaluated. The G. mellonella model showed a clear diversity in the S. aureus pathogenicity pattern, allowing the differentiation of strains with virulence phenotypes ranging from high to low degrees. Histological analysis confirmed that the strains tested were capable of inducing the formation of nodules and melanization spots in the dorsal vessels of the larvae in different magnitudes. The strains 16S-717, 19C-828, and 31S-1443 presented the highest virulence intensity among the bacteria tested and will be used further for the generation of S. aureus mutant populations to prospect genetic targets aimed to develop control strategies of bovine mastitis. Altogether, our results suggest that G. mellonella is an attractive and low-cost animal model for characterizing virulence phenotypes of large S. aureus populations.
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spelling Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis?Infecção intramamáriaVirulência bacterianaModelo de invertebradosBovinoDoença AnimalStaphylococcus aureus is one of the agents of bovine mastitis of hardest control due to a complex pathogenesis comprising a variety of virulence factors, which ensures its persistence in the mammary gland, causing significant health and economic losses. Therefore, understanding the pathogenesis of this agent is imperative. Galleria mellonella has stood out as an invertebrate animal model for the study of infectious diseases that affect several hosts. This work aimed to evaluate G. mellonella larvae as an experimental model for the study of virulence phenotypes in an S. aureus population isolated from bovine mastitis. Thirty genetically divergent S. aureus strains were chosen based on PFGE analysis. After experimental infection, larvae survival rates, bacterial growth in hemolymph, melanization intensity of the dorsal vessel, and histological characteristics of the infected tissues were evaluated. The G. mellonella model showed a clear diversity in the S. aureus pathogenicity pattern, allowing the differentiation of strains with virulence phenotypes ranging from high to low degrees. Histological analysis confirmed that the strains tested were capable of inducing the formation of nodules and melanization spots in the dorsal vessels of the larvae in different magnitudes. The strains 16S-717, 19C-828, and 31S-1443 presented the highest virulence intensity among the bacteria tested and will be used further for the generation of S. aureus mutant populations to prospect genetic targets aimed to develop control strategies of bovine mastitis. Altogether, our results suggest that G. mellonella is an attractive and low-cost animal model for characterizing virulence phenotypes of large S. aureus populations.JULIANA ROSA DA SILVA, UNIVERSIDADE FEDERAL DE LAVRAS; JOICE FÁTIMA MOREIRA SILVA, UNIVERSIDADE FEDERAL DE LAVRAS; MONALESSA FÁBIA PEREIRA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; ADALGISA RIBEIRO TORRES; MAYSA SERPA GONÇALVES, UNIVERSIDADE FEDERAL DE LAVRAS; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; MARIA APARECIDA VASCONCELOS PAIVA E BRITO; GERALDO MÁRCIO DA COSTA, UNIVERSIDADE FEDERAL DE LAVRAS; JOAO BATISTA RIBEIRO, CNPGL.SILVA, J. R. daSILVA, J. F. M.PEREIRA, M. F.TORRES, A. R.GONÇALVES, M. S.PRATA, M. C. de A.BRITO, M. A. V. P. eCOSTA, G. M. daRIBEIRO, J. B.2024-03-11T12:32:31Z2024-03-11T12:32:31Z2023-12-142024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrazilian Journal of Microbiology, v. 55, n. 1, p. 889-900, 2024.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159710https://doi.org/10.1007/s42770-023-01181-1enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2024-03-11T12:32:31Zoai:www.alice.cnptia.embrapa.br:doc/1159710Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542024-03-11T12:32:31falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542024-03-11T12:32:31Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis?
title Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis?
spellingShingle Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis?
SILVA, J. R. da
Infecção intramamária
Virulência bacteriana
Modelo de invertebrados
Bovino
Doença Animal
title_short Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis?
title_full Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis?
title_fullStr Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis?
title_full_unstemmed Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis?
title_sort Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis?
author SILVA, J. R. da
author_facet SILVA, J. R. da
SILVA, J. F. M.
PEREIRA, M. F.
TORRES, A. R.
GONÇALVES, M. S.
PRATA, M. C. de A.
BRITO, M. A. V. P. e
COSTA, G. M. da
RIBEIRO, J. B.
author_role author
author2 SILVA, J. F. M.
PEREIRA, M. F.
TORRES, A. R.
GONÇALVES, M. S.
PRATA, M. C. de A.
BRITO, M. A. V. P. e
COSTA, G. M. da
RIBEIRO, J. B.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv JULIANA ROSA DA SILVA, UNIVERSIDADE FEDERAL DE LAVRAS; JOICE FÁTIMA MOREIRA SILVA, UNIVERSIDADE FEDERAL DE LAVRAS; MONALESSA FÁBIA PEREIRA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; ADALGISA RIBEIRO TORRES; MAYSA SERPA GONÇALVES, UNIVERSIDADE FEDERAL DE LAVRAS; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; MARIA APARECIDA VASCONCELOS PAIVA E BRITO; GERALDO MÁRCIO DA COSTA, UNIVERSIDADE FEDERAL DE LAVRAS; JOAO BATISTA RIBEIRO, CNPGL.
dc.contributor.author.fl_str_mv SILVA, J. R. da
SILVA, J. F. M.
PEREIRA, M. F.
TORRES, A. R.
GONÇALVES, M. S.
PRATA, M. C. de A.
BRITO, M. A. V. P. e
COSTA, G. M. da
RIBEIRO, J. B.
dc.subject.por.fl_str_mv Infecção intramamária
Virulência bacteriana
Modelo de invertebrados
Bovino
Doença Animal
topic Infecção intramamária
Virulência bacteriana
Modelo de invertebrados
Bovino
Doença Animal
description Staphylococcus aureus is one of the agents of bovine mastitis of hardest control due to a complex pathogenesis comprising a variety of virulence factors, which ensures its persistence in the mammary gland, causing significant health and economic losses. Therefore, understanding the pathogenesis of this agent is imperative. Galleria mellonella has stood out as an invertebrate animal model for the study of infectious diseases that affect several hosts. This work aimed to evaluate G. mellonella larvae as an experimental model for the study of virulence phenotypes in an S. aureus population isolated from bovine mastitis. Thirty genetically divergent S. aureus strains were chosen based on PFGE analysis. After experimental infection, larvae survival rates, bacterial growth in hemolymph, melanization intensity of the dorsal vessel, and histological characteristics of the infected tissues were evaluated. The G. mellonella model showed a clear diversity in the S. aureus pathogenicity pattern, allowing the differentiation of strains with virulence phenotypes ranging from high to low degrees. Histological analysis confirmed that the strains tested were capable of inducing the formation of nodules and melanization spots in the dorsal vessels of the larvae in different magnitudes. The strains 16S-717, 19C-828, and 31S-1443 presented the highest virulence intensity among the bacteria tested and will be used further for the generation of S. aureus mutant populations to prospect genetic targets aimed to develop control strategies of bovine mastitis. Altogether, our results suggest that G. mellonella is an attractive and low-cost animal model for characterizing virulence phenotypes of large S. aureus populations.
publishDate 2023
dc.date.none.fl_str_mv 2023-12-14
2024-03-11T12:32:31Z
2024-03-11T12:32:31Z
2024
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Brazilian Journal of Microbiology, v. 55, n. 1, p. 889-900, 2024.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159710
https://doi.org/10.1007/s42770-023-01181-1
identifier_str_mv Brazilian Journal of Microbiology, v. 55, n. 1, p. 889-900, 2024.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159710
https://doi.org/10.1007/s42770-023-01181-1
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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