Impact of oil contamination and biostimulation on the diversity of indigenous bacterial communities in soil microcosms.
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1135450 https://doi.org/10.1016/j.femsec.2004.04.007 |
Resumo: | The aim of this study was to analyse the effect of oil contamination and biostimulation (soil pH raise, and nitrogen, phosphate and sulphur addition) on the diversity of a bacterial community of an acidic Cambisol under Atlantic Forest. The experiment was based on the enumeration of bacterial populations and hydrocarbon degraders in microcosms through the use of conventional plating techniques and molecular fingerprinting of samples directly from the environment. PCR followed by denaturing gradient gel electrophoresis (DGGE) was used to generate microbial community fingerprints employing 16S rRNA gene as molecular marker. Biostimulation led to increases of soil pH (to 7.0) and of the levels of phosphorus and K, Ca, and Mg. Oil contamination caused an increase in soil organic carbon (170-190% higher than control soil). Total bacterial counts were stable throughout the experiment, while MPN counts of hydrocarbon degraders showed an increase in the biostimulated and oil-contaminated soil samples. Molecular fingerprinting performed with 16S rRNA gene PCR and DGGE analysis revealed stable patterns along the 360 days of experiment, showing little change in oil-contaminated microcosms after 90 days. The DGGE patterns of the biostimulated samples showed severe changes due to decreases in the number of bands as compared to the control samples as from 15 days after addition of nutrients to the soil. Results obtained in the present study indicate that the addition of inorganic compounds to soil in conjunction with oil contamination has a greater impact on the bacterial community than oil contamination only. |
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Impact of oil contamination and biostimulation on the diversity of indigenous bacterial communities in soil microcosms.Atlantic forestBacterial diversityBiostimulationCrude oilDGGE16S rRNA geneSoil microcosmsCambisolsThe aim of this study was to analyse the effect of oil contamination and biostimulation (soil pH raise, and nitrogen, phosphate and sulphur addition) on the diversity of a bacterial community of an acidic Cambisol under Atlantic Forest. The experiment was based on the enumeration of bacterial populations and hydrocarbon degraders in microcosms through the use of conventional plating techniques and molecular fingerprinting of samples directly from the environment. PCR followed by denaturing gradient gel electrophoresis (DGGE) was used to generate microbial community fingerprints employing 16S rRNA gene as molecular marker. Biostimulation led to increases of soil pH (to 7.0) and of the levels of phosphorus and K, Ca, and Mg. Oil contamination caused an increase in soil organic carbon (170-190% higher than control soil). Total bacterial counts were stable throughout the experiment, while MPN counts of hydrocarbon degraders showed an increase in the biostimulated and oil-contaminated soil samples. Molecular fingerprinting performed with 16S rRNA gene PCR and DGGE analysis revealed stable patterns along the 360 days of experiment, showing little change in oil-contaminated microcosms after 90 days. The DGGE patterns of the biostimulated samples showed severe changes due to decreases in the number of bands as compared to the control samples as from 15 days after addition of nutrients to the soil. Results obtained in the present study indicate that the addition of inorganic compounds to soil in conjunction with oil contamination has a greater impact on the bacterial community than oil contamination only.Na publicação: Pedro L. O. A. Machado.FLAVIA F. EVANS, UFRJ; ALEXANDRE S. ROSADO, UFRJ; GINA V. SEBASTIÁN, CENPES/PETROBRAS; RENATA CASELLA, CENPES/PETROBRAS; PEDRO LUIZ OLIVEIRA DE A MACHADO, CNPS; CAROLA HOLMSTRÖM, UNIVERSITY OF NEW SOUTH WALES; STAFFAN KJELLEBERG, UNIVERSITY OF NEW SOUTH WALES; JAN D. VAN ELSAS, GRONINGEN UNIVERSITY; LUCY SELDIN, UFRJ.EVANS, F. F.ROSADO, A. S.SEBASTIÁN, G. V.CASELLA, R.MACHADO, P. L. O. de A.HOLMSTRÖM, C.KJELLEBERG, S.ELSAS, J. D. vanSELDIN, L.2021-10-20T02:11:34Z2021-10-20T02:11:34Z2021-10-192004info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFEMS Microbiology Ecology, v. 49, n. 2, p. 295-305, Aug. 2004.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1135450https://doi.org/10.1016/j.femsec.2004.04.007enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2021-10-20T02:11:42Zoai:www.alice.cnptia.embrapa.br:doc/1135450Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542021-10-20T02:11:42falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542021-10-20T02:11:42Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Impact of oil contamination and biostimulation on the diversity of indigenous bacterial communities in soil microcosms. |
title |
Impact of oil contamination and biostimulation on the diversity of indigenous bacterial communities in soil microcosms. |
spellingShingle |
Impact of oil contamination and biostimulation on the diversity of indigenous bacterial communities in soil microcosms. EVANS, F. F. Atlantic forest Bacterial diversity Biostimulation Crude oil DGGE 16S rRNA gene Soil microcosms Cambisols |
title_short |
Impact of oil contamination and biostimulation on the diversity of indigenous bacterial communities in soil microcosms. |
title_full |
Impact of oil contamination and biostimulation on the diversity of indigenous bacterial communities in soil microcosms. |
title_fullStr |
Impact of oil contamination and biostimulation on the diversity of indigenous bacterial communities in soil microcosms. |
title_full_unstemmed |
Impact of oil contamination and biostimulation on the diversity of indigenous bacterial communities in soil microcosms. |
title_sort |
Impact of oil contamination and biostimulation on the diversity of indigenous bacterial communities in soil microcosms. |
author |
EVANS, F. F. |
author_facet |
EVANS, F. F. ROSADO, A. S. SEBASTIÁN, G. V. CASELLA, R. MACHADO, P. L. O. de A. HOLMSTRÖM, C. KJELLEBERG, S. ELSAS, J. D. van SELDIN, L. |
author_role |
author |
author2 |
ROSADO, A. S. SEBASTIÁN, G. V. CASELLA, R. MACHADO, P. L. O. de A. HOLMSTRÖM, C. KJELLEBERG, S. ELSAS, J. D. van SELDIN, L. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
FLAVIA F. EVANS, UFRJ; ALEXANDRE S. ROSADO, UFRJ; GINA V. SEBASTIÁN, CENPES/PETROBRAS; RENATA CASELLA, CENPES/PETROBRAS; PEDRO LUIZ OLIVEIRA DE A MACHADO, CNPS; CAROLA HOLMSTRÖM, UNIVERSITY OF NEW SOUTH WALES; STAFFAN KJELLEBERG, UNIVERSITY OF NEW SOUTH WALES; JAN D. VAN ELSAS, GRONINGEN UNIVERSITY; LUCY SELDIN, UFRJ. |
dc.contributor.author.fl_str_mv |
EVANS, F. F. ROSADO, A. S. SEBASTIÁN, G. V. CASELLA, R. MACHADO, P. L. O. de A. HOLMSTRÖM, C. KJELLEBERG, S. ELSAS, J. D. van SELDIN, L. |
dc.subject.por.fl_str_mv |
Atlantic forest Bacterial diversity Biostimulation Crude oil DGGE 16S rRNA gene Soil microcosms Cambisols |
topic |
Atlantic forest Bacterial diversity Biostimulation Crude oil DGGE 16S rRNA gene Soil microcosms Cambisols |
description |
The aim of this study was to analyse the effect of oil contamination and biostimulation (soil pH raise, and nitrogen, phosphate and sulphur addition) on the diversity of a bacterial community of an acidic Cambisol under Atlantic Forest. The experiment was based on the enumeration of bacterial populations and hydrocarbon degraders in microcosms through the use of conventional plating techniques and molecular fingerprinting of samples directly from the environment. PCR followed by denaturing gradient gel electrophoresis (DGGE) was used to generate microbial community fingerprints employing 16S rRNA gene as molecular marker. Biostimulation led to increases of soil pH (to 7.0) and of the levels of phosphorus and K, Ca, and Mg. Oil contamination caused an increase in soil organic carbon (170-190% higher than control soil). Total bacterial counts were stable throughout the experiment, while MPN counts of hydrocarbon degraders showed an increase in the biostimulated and oil-contaminated soil samples. Molecular fingerprinting performed with 16S rRNA gene PCR and DGGE analysis revealed stable patterns along the 360 days of experiment, showing little change in oil-contaminated microcosms after 90 days. The DGGE patterns of the biostimulated samples showed severe changes due to decreases in the number of bands as compared to the control samples as from 15 days after addition of nutrients to the soil. Results obtained in the present study indicate that the addition of inorganic compounds to soil in conjunction with oil contamination has a greater impact on the bacterial community than oil contamination only. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004 2021-10-20T02:11:34Z 2021-10-20T02:11:34Z 2021-10-19 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
FEMS Microbiology Ecology, v. 49, n. 2, p. 295-305, Aug. 2004. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1135450 https://doi.org/10.1016/j.femsec.2004.04.007 |
identifier_str_mv |
FEMS Microbiology Ecology, v. 49, n. 2, p. 295-305, Aug. 2004. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1135450 https://doi.org/10.1016/j.femsec.2004.04.007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503510875701248 |