A Non-synonymous point mutation in a WD-40 domain repeat of EML5 leads to decreased bovine sperm quality and fertility.
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1155065 https://doi.org/10.3389/fcell.2022.872740 |
Resumo: | This study is part of a concerted effort to identify and phenotype rare, deleterious mutations that adversely affect sperm quality, or convey high developmental and fertility potential to embryos and ensuing progeny. A rare, homozygous mutation in EML5 (EML5R1654W), which encodes a microtubule-associated protein with high expression in testis and brain was identified in an Angus bull used extensively in artificial insemination (AI) for its outstanding progeny production traits. The bull?s fertility was low in cross-breeding timed AI (TAI) (Pregnancy/TAI = 25.2%; n = 222) and, in general, AI breeding to Nellore cows (41%; n = 822). A search of the 1,000 Bull Genomes Run9 database revealed an additional 74 heterozygous animals and 8 homozygous animals harboring this exact mutation across several different breeds (0.7% frequency within the 6,191 sequenced animals). Phenotypically, spermatozoa from the homozygous Angus bull displayed prominent piriform and tapered heads, and outwardly protruding knobbed acrosomes. Additionally, an increased retention of EML5 was also observed in the sperm head of both homozygous and heterozygous Angus bulls compared to wild-type animals. This non-synonymous point mutation is located within a WD40 signaling domain repeat of EML5 and is predicted to be detrimental to overall protein function by genomic single nucleotide polymorphism (SNP) analysis and protein modeling. Future work will examine how this rare mutation affects field AI fertility and will characterize the role of EML5 in spermatogenesis. |
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A Non-synonymous point mutation in a WD-40 domain repeat of EML5 leads to decreased bovine sperm quality and fertility.EspermatozóideFertilidade AnimalSpermiogenesisFertilization (reproduction)BiogenesisSpermatozoaAnimal fertilityAcrosomeThis study is part of a concerted effort to identify and phenotype rare, deleterious mutations that adversely affect sperm quality, or convey high developmental and fertility potential to embryos and ensuing progeny. A rare, homozygous mutation in EML5 (EML5R1654W), which encodes a microtubule-associated protein with high expression in testis and brain was identified in an Angus bull used extensively in artificial insemination (AI) for its outstanding progeny production traits. The bull?s fertility was low in cross-breeding timed AI (TAI) (Pregnancy/TAI = 25.2%; n = 222) and, in general, AI breeding to Nellore cows (41%; n = 822). A search of the 1,000 Bull Genomes Run9 database revealed an additional 74 heterozygous animals and 8 homozygous animals harboring this exact mutation across several different breeds (0.7% frequency within the 6,191 sequenced animals). Phenotypically, spermatozoa from the homozygous Angus bull displayed prominent piriform and tapered heads, and outwardly protruding knobbed acrosomes. Additionally, an increased retention of EML5 was also observed in the sperm head of both homozygous and heterozygous Angus bulls compared to wild-type animals. This non-synonymous point mutation is located within a WD40 signaling domain repeat of EML5 and is predicted to be detrimental to overall protein function by genomic single nucleotide polymorphism (SNP) analysis and protein modeling. Future work will examine how this rare mutation affects field AI fertility and will characterize the role of EML5 in spermatogenesis.ERIKLIS NOGUEIRA, CPAP; FILIP TIRPÁK, Slovak University of Agriculture; LAUREN HAMILTON, UNIVERISTY OF MISSOURI; MICHAL ZIGO, UNIVERSITY OF MISSOURI; KARL KERNS, UNIVERSITY OF MISSOURI; MIRIAM SUTOVSKY, UNIVERSITY OF MISSOURI; JAEWOO KIM, UNIVERSITY OF MISSOURI; DIETRICH VOLKMANN, UNIVERSITY OF MISSOURI; LUCA JOVINE, KAROLINSKA INSTITUTET; JEREMY F. TAYLOR, UNIVERSITY OF MISSOURI; ROBERT D. SCHNABEL, UNIVERSITY OF MISSOURI; PETER SUTOVSKY, UNIVERSITY OF MISSOURI.NOGUEIRA, E.TIRPÁK, F.HAMILTON, L.ZIGO, M.KERNS, K.SUTOVSKY, M.KIM, J.VOLKMANN, D.JOVINE, L.TAYLOR, J. F.SCHNABEL, R. D.SUTOVSKY, P.2023-07-19T19:24:03Z2023-07-19T19:24:03Z2023-07-192022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFrontiers in Cell and Developmental Biology, v. 10, 872740, Apr. 2022.2296-634Xhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1155065https://doi.org/10.3389/fcell.2022.872740enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-07-19T19:24:03Zoai:www.alice.cnptia.embrapa.br:doc/1155065Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-07-19T19:24:03falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-07-19T19:24:03Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
A Non-synonymous point mutation in a WD-40 domain repeat of EML5 leads to decreased bovine sperm quality and fertility. |
title |
A Non-synonymous point mutation in a WD-40 domain repeat of EML5 leads to decreased bovine sperm quality and fertility. |
spellingShingle |
A Non-synonymous point mutation in a WD-40 domain repeat of EML5 leads to decreased bovine sperm quality and fertility. NOGUEIRA, E. Espermatozóide Fertilidade Animal Spermiogenesis Fertilization (reproduction) Biogenesis Spermatozoa Animal fertility Acrosome |
title_short |
A Non-synonymous point mutation in a WD-40 domain repeat of EML5 leads to decreased bovine sperm quality and fertility. |
title_full |
A Non-synonymous point mutation in a WD-40 domain repeat of EML5 leads to decreased bovine sperm quality and fertility. |
title_fullStr |
A Non-synonymous point mutation in a WD-40 domain repeat of EML5 leads to decreased bovine sperm quality and fertility. |
title_full_unstemmed |
A Non-synonymous point mutation in a WD-40 domain repeat of EML5 leads to decreased bovine sperm quality and fertility. |
title_sort |
A Non-synonymous point mutation in a WD-40 domain repeat of EML5 leads to decreased bovine sperm quality and fertility. |
author |
NOGUEIRA, E. |
author_facet |
NOGUEIRA, E. TIRPÁK, F. HAMILTON, L. ZIGO, M. KERNS, K. SUTOVSKY, M. KIM, J. VOLKMANN, D. JOVINE, L. TAYLOR, J. F. SCHNABEL, R. D. SUTOVSKY, P. |
author_role |
author |
author2 |
TIRPÁK, F. HAMILTON, L. ZIGO, M. KERNS, K. SUTOVSKY, M. KIM, J. VOLKMANN, D. JOVINE, L. TAYLOR, J. F. SCHNABEL, R. D. SUTOVSKY, P. |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
ERIKLIS NOGUEIRA, CPAP; FILIP TIRPÁK, Slovak University of Agriculture; LAUREN HAMILTON, UNIVERISTY OF MISSOURI; MICHAL ZIGO, UNIVERSITY OF MISSOURI; KARL KERNS, UNIVERSITY OF MISSOURI; MIRIAM SUTOVSKY, UNIVERSITY OF MISSOURI; JAEWOO KIM, UNIVERSITY OF MISSOURI; DIETRICH VOLKMANN, UNIVERSITY OF MISSOURI; LUCA JOVINE, KAROLINSKA INSTITUTET; JEREMY F. TAYLOR, UNIVERSITY OF MISSOURI; ROBERT D. SCHNABEL, UNIVERSITY OF MISSOURI; PETER SUTOVSKY, UNIVERSITY OF MISSOURI. |
dc.contributor.author.fl_str_mv |
NOGUEIRA, E. TIRPÁK, F. HAMILTON, L. ZIGO, M. KERNS, K. SUTOVSKY, M. KIM, J. VOLKMANN, D. JOVINE, L. TAYLOR, J. F. SCHNABEL, R. D. SUTOVSKY, P. |
dc.subject.por.fl_str_mv |
Espermatozóide Fertilidade Animal Spermiogenesis Fertilization (reproduction) Biogenesis Spermatozoa Animal fertility Acrosome |
topic |
Espermatozóide Fertilidade Animal Spermiogenesis Fertilization (reproduction) Biogenesis Spermatozoa Animal fertility Acrosome |
description |
This study is part of a concerted effort to identify and phenotype rare, deleterious mutations that adversely affect sperm quality, or convey high developmental and fertility potential to embryos and ensuing progeny. A rare, homozygous mutation in EML5 (EML5R1654W), which encodes a microtubule-associated protein with high expression in testis and brain was identified in an Angus bull used extensively in artificial insemination (AI) for its outstanding progeny production traits. The bull?s fertility was low in cross-breeding timed AI (TAI) (Pregnancy/TAI = 25.2%; n = 222) and, in general, AI breeding to Nellore cows (41%; n = 822). A search of the 1,000 Bull Genomes Run9 database revealed an additional 74 heterozygous animals and 8 homozygous animals harboring this exact mutation across several different breeds (0.7% frequency within the 6,191 sequenced animals). Phenotypically, spermatozoa from the homozygous Angus bull displayed prominent piriform and tapered heads, and outwardly protruding knobbed acrosomes. Additionally, an increased retention of EML5 was also observed in the sperm head of both homozygous and heterozygous Angus bulls compared to wild-type animals. This non-synonymous point mutation is located within a WD40 signaling domain repeat of EML5 and is predicted to be detrimental to overall protein function by genomic single nucleotide polymorphism (SNP) analysis and protein modeling. Future work will examine how this rare mutation affects field AI fertility and will characterize the role of EML5 in spermatogenesis. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022 2023-07-19T19:24:03Z 2023-07-19T19:24:03Z 2023-07-19 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Frontiers in Cell and Developmental Biology, v. 10, 872740, Apr. 2022. 2296-634X http://www.alice.cnptia.embrapa.br/alice/handle/doc/1155065 https://doi.org/10.3389/fcell.2022.872740 |
identifier_str_mv |
Frontiers in Cell and Developmental Biology, v. 10, 872740, Apr. 2022. 2296-634X |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1155065 https://doi.org/10.3389/fcell.2022.872740 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503547409137664 |