Whitefly-tolerant transgenic common bean (Phaseolus vulgaris) line.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1146488 https://doi.org/10.3389/fpls.2022.984804 |
Resumo: | Common bean (Phaseolus vulgaris L.) is a staple food in Brazil with both nutritional and socioeconomic importance. As an orphan crop, it has not received as much research attention as the commodity crops. Crop losses are strongly related to virus diseases transmitted by the whitefly Bemisia tabaci, one of the most important agricultural pests in the world. The main method of managing whitefly-transmitted viruses has been the application of insecticides to reduce vector populations. Compared to chemical vector control, a more sustainable strategy for managing insect-borne viruses is the development of resistant/tolerant cultivars. RNA interference has been applied to develop plant lines resistant to the whitefly in other species, such as tomato, lettuce and tobacco. Still, no whitefly-resistant plant has been made commercially available to date. Common bean is a recalcitrant species to in vitro regeneration; therefore, stable genetic transformation of this plant has been achieved only at low frequencies (<1%) using particle bombardment. In the present work, two transgenic common bean lines were obtained with an intron-hairpin construct to induce post-transcriptional gene silencing against the B. tabaci vATPase (Bt-vATPase) gene, with stable expression of siRNA. Northern blot analysis revealed the presence of bands of expected size for siRNA in leaf samples of the line Bt-22.5, while in the other line (11.5), the amount of siRNA produced was significantly smaller. Bioassays were conducted with both lines, but only the line Bt-22.5 was associated with significant mortality of adult insects (97% when insects were fed on detached leaves and 59% on the whole plant). The expression of the Bt-vATPase gene was 50% lower (p < 0.05) in insects that fed on the transgenic line Bt-22.5, when compared to non-transgenic controls. The transgenic line did not affect the virus transmission ability of the insects. Moreover, no effect was observe on the reproduction of non-target organisms, such as the black aphid Aphis craccivora, the leafminer Liriomyza sp. and the whitefly parasitoid Encarsia formosa. The results presented here serve as a basis for the development of whitefly-tolerant transgenic elite common bean cultivars, with potential to contribute to the management of the whitefly and virus diseases. |
id |
EMBR_134563d1120029695777cd425e388c9b |
---|---|
oai_identifier_str |
oai:www.alice.cnptia.embrapa.br:doc/1146488 |
network_acronym_str |
EMBR |
network_name_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository_id_str |
2154 |
spelling |
Whitefly-tolerant transgenic common bean (Phaseolus vulgaris) line.Dry beanInsect pest managementVATPaseBemisia TabaciFeijãoPhaseolus VulgarisPlanta TransgênicaMosca BrancaRNA interferenceBeansTransgenic plantsCommon bean (Phaseolus vulgaris L.) is a staple food in Brazil with both nutritional and socioeconomic importance. As an orphan crop, it has not received as much research attention as the commodity crops. Crop losses are strongly related to virus diseases transmitted by the whitefly Bemisia tabaci, one of the most important agricultural pests in the world. The main method of managing whitefly-transmitted viruses has been the application of insecticides to reduce vector populations. Compared to chemical vector control, a more sustainable strategy for managing insect-borne viruses is the development of resistant/tolerant cultivars. RNA interference has been applied to develop plant lines resistant to the whitefly in other species, such as tomato, lettuce and tobacco. Still, no whitefly-resistant plant has been made commercially available to date. Common bean is a recalcitrant species to in vitro regeneration; therefore, stable genetic transformation of this plant has been achieved only at low frequencies (<1%) using particle bombardment. In the present work, two transgenic common bean lines were obtained with an intron-hairpin construct to induce post-transcriptional gene silencing against the B. tabaci vATPase (Bt-vATPase) gene, with stable expression of siRNA. Northern blot analysis revealed the presence of bands of expected size for siRNA in leaf samples of the line Bt-22.5, while in the other line (11.5), the amount of siRNA produced was significantly smaller. Bioassays were conducted with both lines, but only the line Bt-22.5 was associated with significant mortality of adult insects (97% when insects were fed on detached leaves and 59% on the whole plant). The expression of the Bt-vATPase gene was 50% lower (p < 0.05) in insects that fed on the transgenic line Bt-22.5, when compared to non-transgenic controls. The transgenic line did not affect the virus transmission ability of the insects. Moreover, no effect was observe on the reproduction of non-target organisms, such as the black aphid Aphis craccivora, the leafminer Liriomyza sp. and the whitefly parasitoid Encarsia formosa. The results presented here serve as a basis for the development of whitefly-tolerant transgenic elite common bean cultivars, with potential to contribute to the management of the whitefly and virus diseases.AMANDA LOPES FERREIRA, bolsista CNPAF; JOSIAS CORREA DE FARIA, CNPAF; MATHEUS DA COSTA MOURA, UNIVERSIDADE FEDERAL DE GOIÁS; ANTÔNIA LOPES DE MENDONÇA ZAIDEM, UNIVERSIDADE FEDERAL DE GOIÁS; CAROLINA SENHORINHO RAMALHO PIZETTA; ELÍNEA DE OLIVEIRA FREITAS; GESIMARIA RIBEIRO COSTA COELHO, CNPAF; JOSE FRANCISCO ARRUDA E SILVA, CNPAF; JOSE ALEXANDRE F BARRIGOSSI, CNPAF; LUCIA VIEIRA HOFFMANN, CNPA; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF; FRANCISCO JOSE LIMA ARAGAO, Cenargen; PATRICIA VALLE PINHEIRO, CNPAF.FERREIRA, A. L.FARIA, J. C. deMOURA, M. da C.ZAIDEM, A. L. de M.PIZETTA, C. S. R.FREITAS, E. de O.COELHO, G. R. C.SILVA, J. F. A. eBARRIGOSSI, J. A. F.HOFFMANN, L. V.SOUZA, T. L. P. O. deARAGÃO, F. J. L.PINHEIRO, P. V.2022-09-15T19:05:55Z2022-09-15T19:05:55Z2022-09-152022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFrontiers in Plant Science, v. 13, 984804, Aug. 2022.1664-462Xhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1146488https://doi.org/10.3389/fpls.2022.984804enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2022-09-15T19:06:04Zoai:www.alice.cnptia.embrapa.br:doc/1146488Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542022-09-15T19:06:04falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542022-09-15T19:06:04Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Whitefly-tolerant transgenic common bean (Phaseolus vulgaris) line. |
title |
Whitefly-tolerant transgenic common bean (Phaseolus vulgaris) line. |
spellingShingle |
Whitefly-tolerant transgenic common bean (Phaseolus vulgaris) line. FERREIRA, A. L. Dry bean Insect pest management VATPase Bemisia Tabaci Feijão Phaseolus Vulgaris Planta Transgênica Mosca Branca RNA interference Beans Transgenic plants |
title_short |
Whitefly-tolerant transgenic common bean (Phaseolus vulgaris) line. |
title_full |
Whitefly-tolerant transgenic common bean (Phaseolus vulgaris) line. |
title_fullStr |
Whitefly-tolerant transgenic common bean (Phaseolus vulgaris) line. |
title_full_unstemmed |
Whitefly-tolerant transgenic common bean (Phaseolus vulgaris) line. |
title_sort |
Whitefly-tolerant transgenic common bean (Phaseolus vulgaris) line. |
author |
FERREIRA, A. L. |
author_facet |
FERREIRA, A. L. FARIA, J. C. de MOURA, M. da C. ZAIDEM, A. L. de M. PIZETTA, C. S. R. FREITAS, E. de O. COELHO, G. R. C. SILVA, J. F. A. e BARRIGOSSI, J. A. F. HOFFMANN, L. V. SOUZA, T. L. P. O. de ARAGÃO, F. J. L. PINHEIRO, P. V. |
author_role |
author |
author2 |
FARIA, J. C. de MOURA, M. da C. ZAIDEM, A. L. de M. PIZETTA, C. S. R. FREITAS, E. de O. COELHO, G. R. C. SILVA, J. F. A. e BARRIGOSSI, J. A. F. HOFFMANN, L. V. SOUZA, T. L. P. O. de ARAGÃO, F. J. L. PINHEIRO, P. V. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
AMANDA LOPES FERREIRA, bolsista CNPAF; JOSIAS CORREA DE FARIA, CNPAF; MATHEUS DA COSTA MOURA, UNIVERSIDADE FEDERAL DE GOIÁS; ANTÔNIA LOPES DE MENDONÇA ZAIDEM, UNIVERSIDADE FEDERAL DE GOIÁS; CAROLINA SENHORINHO RAMALHO PIZETTA; ELÍNEA DE OLIVEIRA FREITAS; GESIMARIA RIBEIRO COSTA COELHO, CNPAF; JOSE FRANCISCO ARRUDA E SILVA, CNPAF; JOSE ALEXANDRE F BARRIGOSSI, CNPAF; LUCIA VIEIRA HOFFMANN, CNPA; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF; FRANCISCO JOSE LIMA ARAGAO, Cenargen; PATRICIA VALLE PINHEIRO, CNPAF. |
dc.contributor.author.fl_str_mv |
FERREIRA, A. L. FARIA, J. C. de MOURA, M. da C. ZAIDEM, A. L. de M. PIZETTA, C. S. R. FREITAS, E. de O. COELHO, G. R. C. SILVA, J. F. A. e BARRIGOSSI, J. A. F. HOFFMANN, L. V. SOUZA, T. L. P. O. de ARAGÃO, F. J. L. PINHEIRO, P. V. |
dc.subject.por.fl_str_mv |
Dry bean Insect pest management VATPase Bemisia Tabaci Feijão Phaseolus Vulgaris Planta Transgênica Mosca Branca RNA interference Beans Transgenic plants |
topic |
Dry bean Insect pest management VATPase Bemisia Tabaci Feijão Phaseolus Vulgaris Planta Transgênica Mosca Branca RNA interference Beans Transgenic plants |
description |
Common bean (Phaseolus vulgaris L.) is a staple food in Brazil with both nutritional and socioeconomic importance. As an orphan crop, it has not received as much research attention as the commodity crops. Crop losses are strongly related to virus diseases transmitted by the whitefly Bemisia tabaci, one of the most important agricultural pests in the world. The main method of managing whitefly-transmitted viruses has been the application of insecticides to reduce vector populations. Compared to chemical vector control, a more sustainable strategy for managing insect-borne viruses is the development of resistant/tolerant cultivars. RNA interference has been applied to develop plant lines resistant to the whitefly in other species, such as tomato, lettuce and tobacco. Still, no whitefly-resistant plant has been made commercially available to date. Common bean is a recalcitrant species to in vitro regeneration; therefore, stable genetic transformation of this plant has been achieved only at low frequencies (<1%) using particle bombardment. In the present work, two transgenic common bean lines were obtained with an intron-hairpin construct to induce post-transcriptional gene silencing against the B. tabaci vATPase (Bt-vATPase) gene, with stable expression of siRNA. Northern blot analysis revealed the presence of bands of expected size for siRNA in leaf samples of the line Bt-22.5, while in the other line (11.5), the amount of siRNA produced was significantly smaller. Bioassays were conducted with both lines, but only the line Bt-22.5 was associated with significant mortality of adult insects (97% when insects were fed on detached leaves and 59% on the whole plant). The expression of the Bt-vATPase gene was 50% lower (p < 0.05) in insects that fed on the transgenic line Bt-22.5, when compared to non-transgenic controls. The transgenic line did not affect the virus transmission ability of the insects. Moreover, no effect was observe on the reproduction of non-target organisms, such as the black aphid Aphis craccivora, the leafminer Liriomyza sp. and the whitefly parasitoid Encarsia formosa. The results presented here serve as a basis for the development of whitefly-tolerant transgenic elite common bean cultivars, with potential to contribute to the management of the whitefly and virus diseases. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-09-15T19:05:55Z 2022-09-15T19:05:55Z 2022-09-15 2022 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Frontiers in Plant Science, v. 13, 984804, Aug. 2022. 1664-462X http://www.alice.cnptia.embrapa.br/alice/handle/doc/1146488 https://doi.org/10.3389/fpls.2022.984804 |
identifier_str_mv |
Frontiers in Plant Science, v. 13, 984804, Aug. 2022. 1664-462X |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1146488 https://doi.org/10.3389/fpls.2022.984804 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1794503531168792576 |