Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks (Capra hircus).
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
| Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1120171 |
Resumo: | Abstract: The Saanen goat breed has been widely explored in breeding programmes; however, there are few reports about the breed?s genetic and molecular composition. Thus, this study aimed to characterize the proteomic profile of spermatozoa from Saanen breeding goats. Five breeding animals with proven fertility were selected, the spermatozoa were collected, and the protein was extracted. Subsequently, the proteins were separated and analysed by two-dimensional electrophoresis and mass spectrometry; the proteins were then identified with the SwissProt database. A total of 31 proteins involved in reproduction were identified, including binding proteins on spermatozoa for fusion with the egg, acrosomal membrane proteins, metabolic enzymes, heat shock proteins, cytoskeletal proteins and spermatozoa motility proteins. The characterization of such proteins clarifies the molecular mechanisms of spermatogenesis and the modifications that ensure the success of fertilization. |
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Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks (Capra hircus).SpermProfilesProteomic profileBrasilBrazilian NortheastCaprinoGoatsSaanenProteomicsSpermatogenesisSemiarid zonesAbstract: The Saanen goat breed has been widely explored in breeding programmes; however, there are few reports about the breed?s genetic and molecular composition. Thus, this study aimed to characterize the proteomic profile of spermatozoa from Saanen breeding goats. Five breeding animals with proven fertility were selected, the spermatozoa were collected, and the protein was extracted. Subsequently, the proteins were separated and analysed by two-dimensional electrophoresis and mass spectrometry; the proteins were then identified with the SwissProt database. A total of 31 proteins involved in reproduction were identified, including binding proteins on spermatozoa for fusion with the egg, acrosomal membrane proteins, metabolic enzymes, heat shock proteins, cytoskeletal proteins and spermatozoa motility proteins. The characterization of such proteins clarifies the molecular mechanisms of spermatogenesis and the modifications that ensure the success of fertilization.TATIANA MARIA FARIAS PINTO, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; RAULZITO FERNANDES MOREIRA, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; MARIA NAGILA CARNEIRO MATOS, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; VITÓRIA VIRGINIA MAGALHÃES SOARES, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; MÔNICA VALERIA DE ALMEIDA AGUIAR, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; PAULO DE TARSO TELES DOURADO DE ARAGÃO, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; JOÃO GARCIA ALVES FILHO, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; FREDERICO BRUNO MENDES BATISTA MORENO, Universidade de Fortaleza (UNIFOR) - Fortaleza, CE, Brasil; ANA CRISTINA DE OLIVEIRA MONTEIRO-MOREIRA, Universidade de Fortaleza (UNIFOR) - Fortaleza, CE, Brasil; CÍNTIA RENATA ROCHA COSTA, Universidade Federal de Pernambuco Federal (UFPE) - Recife, PE, Brasil; JOSÉ LUIZ DE LIMA FILHO, Universidade Federal de Pernambuco Federal (UFPE) - Recife, PE, Brasil; ANGELA MARIA XAVIER ELOY, CNPC; RODRIGO MARANGUAPE SILVA DA CUNHA, Universidade Federal de Pernambuco Federal (UFPE) - Recife, PE, Brasil.PINTO, T. M. F.MOREIRA, R. F.MATOS, M. N. C.SOARES, V. V. M.AGUIAR, M. V. de A.ARAGÃO, P. de T. T. D. deALVES FILHO, J. G.MORENO, F. B. M. B.MONTEIRO-MOREIRA, A. C. de O.COSTA, C. R. R.LIMA FILHO, J. L. deELOY, A. M. X.CUNHA, R. M. S. da2020-02-12T18:09:12Z2020-02-12T18:09:12Z2020-02-1120192020-02-12T18:09:12Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAnimal Reproduction, v. 16, n. 4, p. 902-913, 2019.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1120171enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-02-12T18:09:18Zoai:www.alice.cnptia.embrapa.br:doc/1120171Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542020-02-12T18:09:18falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-02-12T18:09:18Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
| dc.title.none.fl_str_mv |
Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks (Capra hircus). |
| title |
Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks (Capra hircus). |
| spellingShingle |
Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks (Capra hircus). PINTO, T. M. F. Sperm Profiles Proteomic profile Brasil Brazilian Northeast Caprino Goats Saanen Proteomics Spermatogenesis Semiarid zones |
| title_short |
Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks (Capra hircus). |
| title_full |
Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks (Capra hircus). |
| title_fullStr |
Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks (Capra hircus). |
| title_full_unstemmed |
Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks (Capra hircus). |
| title_sort |
Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks (Capra hircus). |
| author |
PINTO, T. M. F. |
| author_facet |
PINTO, T. M. F. MOREIRA, R. F. MATOS, M. N. C. SOARES, V. V. M. AGUIAR, M. V. de A. ARAGÃO, P. de T. T. D. de ALVES FILHO, J. G. MORENO, F. B. M. B. MONTEIRO-MOREIRA, A. C. de O. COSTA, C. R. R. LIMA FILHO, J. L. de ELOY, A. M. X. CUNHA, R. M. S. da |
| author_role |
author |
| author2 |
MOREIRA, R. F. MATOS, M. N. C. SOARES, V. V. M. AGUIAR, M. V. de A. ARAGÃO, P. de T. T. D. de ALVES FILHO, J. G. MORENO, F. B. M. B. MONTEIRO-MOREIRA, A. C. de O. COSTA, C. R. R. LIMA FILHO, J. L. de ELOY, A. M. X. CUNHA, R. M. S. da |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
TATIANA MARIA FARIAS PINTO, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; RAULZITO FERNANDES MOREIRA, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; MARIA NAGILA CARNEIRO MATOS, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; VITÓRIA VIRGINIA MAGALHÃES SOARES, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; MÔNICA VALERIA DE ALMEIDA AGUIAR, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; PAULO DE TARSO TELES DOURADO DE ARAGÃO, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; JOÃO GARCIA ALVES FILHO, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; FREDERICO BRUNO MENDES BATISTA MORENO, Universidade de Fortaleza (UNIFOR) - Fortaleza, CE, Brasil; ANA CRISTINA DE OLIVEIRA MONTEIRO-MOREIRA, Universidade de Fortaleza (UNIFOR) - Fortaleza, CE, Brasil; CÍNTIA RENATA ROCHA COSTA, Universidade Federal de Pernambuco Federal (UFPE) - Recife, PE, Brasil; JOSÉ LUIZ DE LIMA FILHO, Universidade Federal de Pernambuco Federal (UFPE) - Recife, PE, Brasil; ANGELA MARIA XAVIER ELOY, CNPC; RODRIGO MARANGUAPE SILVA DA CUNHA, Universidade Federal de Pernambuco Federal (UFPE) - Recife, PE, Brasil. |
| dc.contributor.author.fl_str_mv |
PINTO, T. M. F. MOREIRA, R. F. MATOS, M. N. C. SOARES, V. V. M. AGUIAR, M. V. de A. ARAGÃO, P. de T. T. D. de ALVES FILHO, J. G. MORENO, F. B. M. B. MONTEIRO-MOREIRA, A. C. de O. COSTA, C. R. R. LIMA FILHO, J. L. de ELOY, A. M. X. CUNHA, R. M. S. da |
| dc.subject.por.fl_str_mv |
Sperm Profiles Proteomic profile Brasil Brazilian Northeast Caprino Goats Saanen Proteomics Spermatogenesis Semiarid zones |
| topic |
Sperm Profiles Proteomic profile Brasil Brazilian Northeast Caprino Goats Saanen Proteomics Spermatogenesis Semiarid zones |
| description |
Abstract: The Saanen goat breed has been widely explored in breeding programmes; however, there are few reports about the breed?s genetic and molecular composition. Thus, this study aimed to characterize the proteomic profile of spermatozoa from Saanen breeding goats. Five breeding animals with proven fertility were selected, the spermatozoa were collected, and the protein was extracted. Subsequently, the proteins were separated and analysed by two-dimensional electrophoresis and mass spectrometry; the proteins were then identified with the SwissProt database. A total of 31 proteins involved in reproduction were identified, including binding proteins on spermatozoa for fusion with the egg, acrosomal membrane proteins, metabolic enzymes, heat shock proteins, cytoskeletal proteins and spermatozoa motility proteins. The characterization of such proteins clarifies the molecular mechanisms of spermatogenesis and the modifications that ensure the success of fertilization. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019 2020-02-12T18:09:12Z 2020-02-12T18:09:12Z 2020-02-11 2020-02-12T18:09:12Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
Animal Reproduction, v. 16, n. 4, p. 902-913, 2019. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1120171 |
| identifier_str_mv |
Animal Reproduction, v. 16, n. 4, p. 902-913, 2019. |
| url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1120171 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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cg-riaa@embrapa.br |
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