Transcriptome analysis identifies candidate target genes involved in glyphosate-resistance mechanism in lolium multiflorum.

Detalhes bibliográficos
Autor(a) principal: CECHIN, J.
Data de Publicação: 2020
Outros Autores: PIASECKI, C., BENEMANN, D. P., KREMER, F. S., GALLI, V., MAIA, L. C., AGOSTINETTO, D., VARGAS, L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1129871
Resumo: Italian ryegrass (Lolium multiflorum; LOLMU) is one of the most troublesome weeds in temperate regions in the world. This weed species interfere with wheat, corn, rye, and oat, causing significant crop yield losses. This species has evolved glyphosate resistance, making it difcult to control. The mechanisms of glyphosate resistance are still unknown, and an understanding thereof will favor the development of new strategies of management. The present study is the first transcriptome study in LOLMU using glyphosate-resistant and -sensitive biotypes, aiming to identify and to provide a list of the candidate target genes related to glyphosate resistance mechanism. The transcriptome was assembled de novo, producing 87,433 contigs with an N50 of 740 bp and an average length of 575 bp. There were 92 and 54 up- and down-regulated genes, respectively, in the resistant biotype, while a total of 1683 were diferentially expressed in the sensitive biotype in response to glyphosate treatment. We selected 14 highly induced genes and seven with repressed expression in the resistant biotype in response to glyphosate. Of these genes, a significant proportion were related to the plasma membrane, indicating that there is a barrier making it difcult for glyphosate to enter the cell.
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spelling Transcriptome analysis identifies candidate target genes involved in glyphosate-resistance mechanism in lolium multiflorum.Italian ryegrassNext-generation sequencingRNA-SeqDiferential gene expressionResistance mechanismItalian ryegrass (Lolium multiflorum; LOLMU) is one of the most troublesome weeds in temperate regions in the world. This weed species interfere with wheat, corn, rye, and oat, causing significant crop yield losses. This species has evolved glyphosate resistance, making it difcult to control. The mechanisms of glyphosate resistance are still unknown, and an understanding thereof will favor the development of new strategies of management. The present study is the first transcriptome study in LOLMU using glyphosate-resistant and -sensitive biotypes, aiming to identify and to provide a list of the candidate target genes related to glyphosate resistance mechanism. The transcriptome was assembled de novo, producing 87,433 contigs with an N50 of 740 bp and an average length of 575 bp. There were 92 and 54 up- and down-regulated genes, respectively, in the resistant biotype, while a total of 1683 were diferentially expressed in the sensitive biotype in response to glyphosate treatment. We selected 14 highly induced genes and seven with repressed expression in the resistant biotype in response to glyphosate. Of these genes, a significant proportion were related to the plasma membrane, indicating that there is a barrier making it difcult for glyphosate to enter the cell.JOANEI CECHIN, Department of Crop Protection, Federal University of Pelotas, Pelotas, RS 96160-000, Brazil; jcechin@ufpel.edu.br (J.C.); daiane.benemann@ufpel.edu.br (D.P.B.); dirceu_agostinetto@ufpel.edu.br (D.A.); CRISTIANO PIASECKI, Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996, USA; DAIANE P. BENEANNM, Department of Crop Protection, Federal University of Pelotas, Pelotas, RS 96160-000, Brazil; jcechin@ufpel.edu.br (J.C.); daiane.benemann@ufpel.edu.br (D.P.B.); dirceu_agostinetto@ufpel.edu.br (D.A.); FREDERICO S. KREMER, Center for Technological Development, Federal University of Pelotas (UFPel), Pelotas, RS 96010-610, Brazil; frederico.kremer@thrivedatascience.com (F.S.K.); vanessa.galli@ufpel.edu.br (V.G.); VANESSA GALLI, Center for Technological Development, Federal University of Pelotas (UFPel), Pelotas, RS 96010-610, Brazil; frederico.kremer@thrivedatascience.com (F.S.K.); vanessa.galli@ufpel.edu.br (V.G.); LUCIANO C. MAIA, Department of Plant Breeding, Federal University of Pelotas (UFPel), Pelotas, RS 96010-610, Brazil; luciano.maia@ufpel.edu.br; DIRCEU AGOSTINETTO, Department of Crop Protection, Federal University of Pelotas, Pelotas, RS 96160-000, Brazil; jcechin@ufpel.edu.br (J.C.); daiane.benemann@ufpel.edu.br (D.P.B.); dirceu_agostinetto@ufpel.edu.br (D.A.); LEANDRO VARGAS, CNPT.CECHIN, J.PIASECKI, C.BENEMANN, D. P.KREMER, F. S.GALLI, V.MAIA, L. C.AGOSTINETTO, D.VARGAS, L.2021-02-09T13:17:50Z2021-02-09T13:17:50Z2021-02-092020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlants, v. 9, p. 685, 2020.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1129871enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2021-02-09T13:18:00Zoai:www.alice.cnptia.embrapa.br:doc/1129871Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542021-02-09T13:18falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542021-02-09T13:18Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Transcriptome analysis identifies candidate target genes involved in glyphosate-resistance mechanism in lolium multiflorum.
title Transcriptome analysis identifies candidate target genes involved in glyphosate-resistance mechanism in lolium multiflorum.
spellingShingle Transcriptome analysis identifies candidate target genes involved in glyphosate-resistance mechanism in lolium multiflorum.
CECHIN, J.
Italian ryegrass
Next-generation sequencing
RNA-Seq
Diferential gene expression
Resistance mechanism
title_short Transcriptome analysis identifies candidate target genes involved in glyphosate-resistance mechanism in lolium multiflorum.
title_full Transcriptome analysis identifies candidate target genes involved in glyphosate-resistance mechanism in lolium multiflorum.
title_fullStr Transcriptome analysis identifies candidate target genes involved in glyphosate-resistance mechanism in lolium multiflorum.
title_full_unstemmed Transcriptome analysis identifies candidate target genes involved in glyphosate-resistance mechanism in lolium multiflorum.
title_sort Transcriptome analysis identifies candidate target genes involved in glyphosate-resistance mechanism in lolium multiflorum.
author CECHIN, J.
author_facet CECHIN, J.
PIASECKI, C.
BENEMANN, D. P.
KREMER, F. S.
GALLI, V.
MAIA, L. C.
AGOSTINETTO, D.
VARGAS, L.
author_role author
author2 PIASECKI, C.
BENEMANN, D. P.
KREMER, F. S.
GALLI, V.
MAIA, L. C.
AGOSTINETTO, D.
VARGAS, L.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv JOANEI CECHIN, Department of Crop Protection, Federal University of Pelotas, Pelotas, RS 96160-000, Brazil; jcechin@ufpel.edu.br (J.C.); daiane.benemann@ufpel.edu.br (D.P.B.); dirceu_agostinetto@ufpel.edu.br (D.A.); CRISTIANO PIASECKI, Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996, USA; DAIANE P. BENEANNM, Department of Crop Protection, Federal University of Pelotas, Pelotas, RS 96160-000, Brazil; jcechin@ufpel.edu.br (J.C.); daiane.benemann@ufpel.edu.br (D.P.B.); dirceu_agostinetto@ufpel.edu.br (D.A.); FREDERICO S. KREMER, Center for Technological Development, Federal University of Pelotas (UFPel), Pelotas, RS 96010-610, Brazil; frederico.kremer@thrivedatascience.com (F.S.K.); vanessa.galli@ufpel.edu.br (V.G.); VANESSA GALLI, Center for Technological Development, Federal University of Pelotas (UFPel), Pelotas, RS 96010-610, Brazil; frederico.kremer@thrivedatascience.com (F.S.K.); vanessa.galli@ufpel.edu.br (V.G.); LUCIANO C. MAIA, Department of Plant Breeding, Federal University of Pelotas (UFPel), Pelotas, RS 96010-610, Brazil; luciano.maia@ufpel.edu.br; DIRCEU AGOSTINETTO, Department of Crop Protection, Federal University of Pelotas, Pelotas, RS 96160-000, Brazil; jcechin@ufpel.edu.br (J.C.); daiane.benemann@ufpel.edu.br (D.P.B.); dirceu_agostinetto@ufpel.edu.br (D.A.); LEANDRO VARGAS, CNPT.
dc.contributor.author.fl_str_mv CECHIN, J.
PIASECKI, C.
BENEMANN, D. P.
KREMER, F. S.
GALLI, V.
MAIA, L. C.
AGOSTINETTO, D.
VARGAS, L.
dc.subject.por.fl_str_mv Italian ryegrass
Next-generation sequencing
RNA-Seq
Diferential gene expression
Resistance mechanism
topic Italian ryegrass
Next-generation sequencing
RNA-Seq
Diferential gene expression
Resistance mechanism
description Italian ryegrass (Lolium multiflorum; LOLMU) is one of the most troublesome weeds in temperate regions in the world. This weed species interfere with wheat, corn, rye, and oat, causing significant crop yield losses. This species has evolved glyphosate resistance, making it difcult to control. The mechanisms of glyphosate resistance are still unknown, and an understanding thereof will favor the development of new strategies of management. The present study is the first transcriptome study in LOLMU using glyphosate-resistant and -sensitive biotypes, aiming to identify and to provide a list of the candidate target genes related to glyphosate resistance mechanism. The transcriptome was assembled de novo, producing 87,433 contigs with an N50 of 740 bp and an average length of 575 bp. There were 92 and 54 up- and down-regulated genes, respectively, in the resistant biotype, while a total of 1683 were diferentially expressed in the sensitive biotype in response to glyphosate treatment. We selected 14 highly induced genes and seven with repressed expression in the resistant biotype in response to glyphosate. Of these genes, a significant proportion were related to the plasma membrane, indicating that there is a barrier making it difcult for glyphosate to enter the cell.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021-02-09T13:17:50Z
2021-02-09T13:17:50Z
2021-02-09
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Plants, v. 9, p. 685, 2020.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1129871
identifier_str_mv Plants, v. 9, p. 685, 2020.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1129871
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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