Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted Functional markers for stress response.

Detalhes bibliográficos
Autor(a) principal: SILVA, C. C.
Data de Publicação: 2014
Outros Autores: MANTELLO, C. C., CAMPOS, T. de, SOUZA, L. M., GONÇALVES, P. S., SOUZA, A. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/988256
Resumo: Hevea brasiliensis is a native species of the Amazon Basin of South America and the primary source of natural rubber worldwide. Due to the occurrence of South American Leaf Blight disease in this area, rubber plantations have been extended to suboptimal regions. Rubber tree breeding is time-consuming and expensive, but molecular markers can serve as a tool for early valuation, thus reducing time and costs. In this work, we constructed six different cDNA libraries with the aim of developing gene-targeted molecular markers for the rubber tree. A total of 8,263 reads were assembled, generating 5,025 unigenes that were analyzed; 912 expressed sequence tags (ESTs) represented new transcripts, and two sequences were highly up-regulated by cold stress. These unigenes were scanned for microsatellite (SSR) regions and single nucleotide polymorphisms (SNPs). In total, 169 novel EST-SSR markers were developed; 138 loci were polymorphic in the rubber tree, and 98 % presented transferability to six other Hevea species. Locus duplication was observed in H. brasil-iensis and other species. Additionally, 43 SNP markers in 13 sequences that showed similarity to proteins involved in stress response, latex biosynthesis and developmental processes were characterized. cDNA libraries are a rich source of SSR and SNP markers and enable the identification of new transcripts. The new markers developed here will be a valuable resource for linkage mapping, QTL identification and other studies in the rubber tree and can also be used to evaluate the genetic variability of other Hevea species, which are valuable assets in rubber tree breeding.
id EMBR_1885a78b5c6763c8c38adca72f18e5da
oai_identifier_str oai:www.alice.cnptia.embrapa.br:doc/988256
network_acronym_str EMBR
network_name_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository_id_str 2154
spelling Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted Functional markers for stress response.Marcador SNPRubber treeMarcador microssatéliteBiblioteca cDNACDNA libraryFitomejoramientoMarcadores genéticosRepeticiones de microsatéliteMelhoramento genético vegetalSeringueiraHevea brasiliensisMarcador genéticoDNACauchoPlant breedingGenetic markersMicrosatellite repeatsHevea brasiliensis is a native species of the Amazon Basin of South America and the primary source of natural rubber worldwide. Due to the occurrence of South American Leaf Blight disease in this area, rubber plantations have been extended to suboptimal regions. Rubber tree breeding is time-consuming and expensive, but molecular markers can serve as a tool for early valuation, thus reducing time and costs. In this work, we constructed six different cDNA libraries with the aim of developing gene-targeted molecular markers for the rubber tree. A total of 8,263 reads were assembled, generating 5,025 unigenes that were analyzed; 912 expressed sequence tags (ESTs) represented new transcripts, and two sequences were highly up-regulated by cold stress. These unigenes were scanned for microsatellite (SSR) regions and single nucleotide polymorphisms (SNPs). In total, 169 novel EST-SSR markers were developed; 138 loci were polymorphic in the rubber tree, and 98 % presented transferability to six other Hevea species. Locus duplication was observed in H. brasil-iensis and other species. Additionally, 43 SNP markers in 13 sequences that showed similarity to proteins involved in stress response, latex biosynthesis and developmental processes were characterized. cDNA libraries are a rich source of SSR and SNP markers and enable the identification of new transcripts. The new markers developed here will be a valuable resource for linkage mapping, QTL identification and other studies in the rubber tree and can also be used to evaluate the genetic variability of other Hevea species, which are valuable assets in rubber tree breeding.Carla C. Silva, Universidade Estadual de Campinas (Unicamp); Camila C. Mantello, Universidade Estadual de Campinas (Unicamp); TATIANA DE CAMPOS, CPAF-AC; Livia M. Souza, Universidade Estadual de Campinas (Unicamp); Paulo S. Gonçalves, Instituto Agronômico de Campinas (IAC); Anete P. Souza, Universidade Estadual de Campinas (Unicamp).SILVA, C. C.MANTELLO, C. C.CAMPOS, T. deSOUZA, L. M.GONÇALVES, P. S.SOUZA, A. P.2014-06-11T11:11:11Z2014-06-11T11:11:11Z2014-06-1120142019-10-29T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMolecular Breeding, Berlim, v. 34, n. 3, p. 1035-1053, Oct. 2014.1380-3743 (impresso) / 1572-9788 (on-line)http://www.alice.cnptia.embrapa.br/alice/handle/doc/98825610.1007/s11032-014-0095-2enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T04:29:12Zoai:www.alice.cnptia.embrapa.br:doc/988256Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T04:29:12falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T04:29:12Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted Functional markers for stress response.
title Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted Functional markers for stress response.
spellingShingle Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted Functional markers for stress response.
SILVA, C. C.
Marcador SNP
Rubber tree
Marcador microssatélite
Biblioteca cDNA
CDNA library
Fitomejoramiento
Marcadores genéticos
Repeticiones de microsatélite
Melhoramento genético vegetal
Seringueira
Hevea brasiliensis
Marcador genético
DNA
Caucho
Plant breeding
Genetic markers
Microsatellite repeats
title_short Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted Functional markers for stress response.
title_full Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted Functional markers for stress response.
title_fullStr Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted Functional markers for stress response.
title_full_unstemmed Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted Functional markers for stress response.
title_sort Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted Functional markers for stress response.
author SILVA, C. C.
author_facet SILVA, C. C.
MANTELLO, C. C.
CAMPOS, T. de
SOUZA, L. M.
GONÇALVES, P. S.
SOUZA, A. P.
author_role author
author2 MANTELLO, C. C.
CAMPOS, T. de
SOUZA, L. M.
GONÇALVES, P. S.
SOUZA, A. P.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Carla C. Silva, Universidade Estadual de Campinas (Unicamp); Camila C. Mantello, Universidade Estadual de Campinas (Unicamp); TATIANA DE CAMPOS, CPAF-AC; Livia M. Souza, Universidade Estadual de Campinas (Unicamp); Paulo S. Gonçalves, Instituto Agronômico de Campinas (IAC); Anete P. Souza, Universidade Estadual de Campinas (Unicamp).
dc.contributor.author.fl_str_mv SILVA, C. C.
MANTELLO, C. C.
CAMPOS, T. de
SOUZA, L. M.
GONÇALVES, P. S.
SOUZA, A. P.
dc.subject.por.fl_str_mv Marcador SNP
Rubber tree
Marcador microssatélite
Biblioteca cDNA
CDNA library
Fitomejoramiento
Marcadores genéticos
Repeticiones de microsatélite
Melhoramento genético vegetal
Seringueira
Hevea brasiliensis
Marcador genético
DNA
Caucho
Plant breeding
Genetic markers
Microsatellite repeats
topic Marcador SNP
Rubber tree
Marcador microssatélite
Biblioteca cDNA
CDNA library
Fitomejoramiento
Marcadores genéticos
Repeticiones de microsatélite
Melhoramento genético vegetal
Seringueira
Hevea brasiliensis
Marcador genético
DNA
Caucho
Plant breeding
Genetic markers
Microsatellite repeats
description Hevea brasiliensis is a native species of the Amazon Basin of South America and the primary source of natural rubber worldwide. Due to the occurrence of South American Leaf Blight disease in this area, rubber plantations have been extended to suboptimal regions. Rubber tree breeding is time-consuming and expensive, but molecular markers can serve as a tool for early valuation, thus reducing time and costs. In this work, we constructed six different cDNA libraries with the aim of developing gene-targeted molecular markers for the rubber tree. A total of 8,263 reads were assembled, generating 5,025 unigenes that were analyzed; 912 expressed sequence tags (ESTs) represented new transcripts, and two sequences were highly up-regulated by cold stress. These unigenes were scanned for microsatellite (SSR) regions and single nucleotide polymorphisms (SNPs). In total, 169 novel EST-SSR markers were developed; 138 loci were polymorphic in the rubber tree, and 98 % presented transferability to six other Hevea species. Locus duplication was observed in H. brasil-iensis and other species. Additionally, 43 SNP markers in 13 sequences that showed similarity to proteins involved in stress response, latex biosynthesis and developmental processes were characterized. cDNA libraries are a rich source of SSR and SNP markers and enable the identification of new transcripts. The new markers developed here will be a valuable resource for linkage mapping, QTL identification and other studies in the rubber tree and can also be used to evaluate the genetic variability of other Hevea species, which are valuable assets in rubber tree breeding.
publishDate 2014
dc.date.none.fl_str_mv 2014-06-11T11:11:11Z
2014-06-11T11:11:11Z
2014-06-11
2014
2019-10-29T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Molecular Breeding, Berlim, v. 34, n. 3, p. 1035-1053, Oct. 2014.
1380-3743 (impresso) / 1572-9788 (on-line)
http://www.alice.cnptia.embrapa.br/alice/handle/doc/988256
10.1007/s11032-014-0095-2
identifier_str_mv Molecular Breeding, Berlim, v. 34, n. 3, p. 1035-1053, Oct. 2014.
1380-3743 (impresso) / 1572-9788 (on-line)
10.1007/s11032-014-0095-2
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/988256
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
_version_ 1794503437045465088