Identification of a Simple Sequence Repeat molecular-marker set for large-scale analyses of pear germplasm.

Detalhes bibliográficos
Autor(a) principal: DEQUIGIOVANNI
Data de Publicação: 2012
Outros Autores: RECH, F., GOMES, F. G. G., CEROTTI, I. S., FAORO, I., OLIVEIRA, P. R. D. de, QUECINI, V., RITSCHEL, P. S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/927728
Resumo: Simple Sequence Repeats (SSR) are molecular markers suitable to assess the genetic variation of germplasm resources; however, large-scale SSR use requires protocol optimization. The present work aimed to identify SSR markers, developed for pear and other fruit species that are effective in characterizing pear germplasm collections and in demonstrating their use in providing support for genetic breeding programs. From a total of 62 SSR markers investigated, 23 yielding reproducible and polymorphic patterns were used to genotype a sample of 42 pear accessions of the Brazilian Pear Germplasm Bank (PGB). When compared to these 23 SSR markers, a subset of eleven markers, selected based on He, PIC and PId, was used to distinguish individual accessions and perform cluster analysis with similar efficacy. Genetic diversity analysis clustered the European, Japanese and Chinese accessions in distinct groups. This markers subset constitutes a valuable tool for several applications related to pear genetic resources management and breeding.
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spelling Identification of a Simple Sequence Repeat molecular-marker set for large-scale analyses of pear germplasm.Identificação genéticaDiversidade genéticaSimple Sequence RepeatsMelhoramento genéticoFruticulturaPeraRecurso genéticoVariedadeMarcador MolecularSimple Sequence Repeats (SSR) are molecular markers suitable to assess the genetic variation of germplasm resources; however, large-scale SSR use requires protocol optimization. The present work aimed to identify SSR markers, developed for pear and other fruit species that are effective in characterizing pear germplasm collections and in demonstrating their use in providing support for genetic breeding programs. From a total of 62 SSR markers investigated, 23 yielding reproducible and polymorphic patterns were used to genotype a sample of 42 pear accessions of the Brazilian Pear Germplasm Bank (PGB). When compared to these 23 SSR markers, a subset of eleven markers, selected based on He, PIC and PId, was used to distinguish individual accessions and perform cluster analysis with similar efficacy. Genetic diversity analysis clustered the European, Japanese and Chinese accessions in distinct groups. This markers subset constitutes a valuable tool for several applications related to pear genetic resources management and breeding.Gabriel Dequigiovanni, CNPUV (BOLSISTA); Fernanda Rech, CNPUV (BOLSISTA); Felippe George Gatti Gomes, CNPUV (BOLSISTA); Ivan Somensi Cerotti, CNPUV (BOLSISTA); Ivan Faoro, EPAGRI; PAULO RICARDO DIAS DE OLIVEIRA, CNPUV; VERA MARIA QUECINI, CNPUV; PATRICIA SILVA RITSCHEL, CNPUV.DEQUIGIOVANNIRECH, F.GOMES, F. G. G.CEROTTI, I. S.FAORO, I.OLIVEIRA, P. R. D. deQUECINI, V.RITSCHEL, P. S.2012-07-05T11:11:11Z2012-07-05T11:11:11Z2012-07-0520122012-07-05T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCrop Breeding and Applied Biotechnology, Viçosa, v. 12, n. 2, p. 118-125, abr./jun. 2012.http://www.alice.cnptia.embrapa.br/alice/handle/doc/927728enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T00:38:30Zoai:www.alice.cnptia.embrapa.br:doc/927728Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T00:38:30falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T00:38:30Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Identification of a Simple Sequence Repeat molecular-marker set for large-scale analyses of pear germplasm.
title Identification of a Simple Sequence Repeat molecular-marker set for large-scale analyses of pear germplasm.
spellingShingle Identification of a Simple Sequence Repeat molecular-marker set for large-scale analyses of pear germplasm.
DEQUIGIOVANNI
Identificação genética
Diversidade genética
Simple Sequence Repeats
Melhoramento genético
Fruticultura
Pera
Recurso genético
Variedade
Marcador Molecular
title_short Identification of a Simple Sequence Repeat molecular-marker set for large-scale analyses of pear germplasm.
title_full Identification of a Simple Sequence Repeat molecular-marker set for large-scale analyses of pear germplasm.
title_fullStr Identification of a Simple Sequence Repeat molecular-marker set for large-scale analyses of pear germplasm.
title_full_unstemmed Identification of a Simple Sequence Repeat molecular-marker set for large-scale analyses of pear germplasm.
title_sort Identification of a Simple Sequence Repeat molecular-marker set for large-scale analyses of pear germplasm.
author DEQUIGIOVANNI
author_facet DEQUIGIOVANNI
RECH, F.
GOMES, F. G. G.
CEROTTI, I. S.
FAORO, I.
OLIVEIRA, P. R. D. de
QUECINI, V.
RITSCHEL, P. S.
author_role author
author2 RECH, F.
GOMES, F. G. G.
CEROTTI, I. S.
FAORO, I.
OLIVEIRA, P. R. D. de
QUECINI, V.
RITSCHEL, P. S.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Gabriel Dequigiovanni, CNPUV (BOLSISTA); Fernanda Rech, CNPUV (BOLSISTA); Felippe George Gatti Gomes, CNPUV (BOLSISTA); Ivan Somensi Cerotti, CNPUV (BOLSISTA); Ivan Faoro, EPAGRI; PAULO RICARDO DIAS DE OLIVEIRA, CNPUV; VERA MARIA QUECINI, CNPUV; PATRICIA SILVA RITSCHEL, CNPUV.
dc.contributor.author.fl_str_mv DEQUIGIOVANNI
RECH, F.
GOMES, F. G. G.
CEROTTI, I. S.
FAORO, I.
OLIVEIRA, P. R. D. de
QUECINI, V.
RITSCHEL, P. S.
dc.subject.por.fl_str_mv Identificação genética
Diversidade genética
Simple Sequence Repeats
Melhoramento genético
Fruticultura
Pera
Recurso genético
Variedade
Marcador Molecular
topic Identificação genética
Diversidade genética
Simple Sequence Repeats
Melhoramento genético
Fruticultura
Pera
Recurso genético
Variedade
Marcador Molecular
description Simple Sequence Repeats (SSR) are molecular markers suitable to assess the genetic variation of germplasm resources; however, large-scale SSR use requires protocol optimization. The present work aimed to identify SSR markers, developed for pear and other fruit species that are effective in characterizing pear germplasm collections and in demonstrating their use in providing support for genetic breeding programs. From a total of 62 SSR markers investigated, 23 yielding reproducible and polymorphic patterns were used to genotype a sample of 42 pear accessions of the Brazilian Pear Germplasm Bank (PGB). When compared to these 23 SSR markers, a subset of eleven markers, selected based on He, PIC and PId, was used to distinguish individual accessions and perform cluster analysis with similar efficacy. Genetic diversity analysis clustered the European, Japanese and Chinese accessions in distinct groups. This markers subset constitutes a valuable tool for several applications related to pear genetic resources management and breeding.
publishDate 2012
dc.date.none.fl_str_mv 2012-07-05T11:11:11Z
2012-07-05T11:11:11Z
2012-07-05
2012
2012-07-05T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Crop Breeding and Applied Biotechnology, Viçosa, v. 12, n. 2, p. 118-125, abr./jun. 2012.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/927728
identifier_str_mv Crop Breeding and Applied Biotechnology, Viçosa, v. 12, n. 2, p. 118-125, abr./jun. 2012.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/927728
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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