Immature embryo rescue and in vitro development evaluation of intraspecific hybrids from Brazilian seedless grapevine Superior × Thompson clones.
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/988687 |
Resumo: | The fruit production for export is an economically significant activity in the Valley of São Francisco River, especially in the irrigated lands of Petrolina-PE/Juazeiro-BA, Brazil. The development of new genetic material most suitable to the tropical climate and the demands of the consumer market have led to the selection of new seedless grapes cultivars. In this case, the use of the embryo rescue technique has produced satisfactory results for obtaining such materials, especially in the semiarid region. This study aimed to evaluate the in vitro development of intraspecific hybrids of grapevine (Vitis vinifera L.), derived from the rescue of immature embryos resultant from the crossing of Superior Seedless and Thompson Seedless Brazilian clones. To establish and develop the cultivation, the culture media was supplemented with 30 g/L sucrose, 0.1 g/L myo-inositol, 0.002 g/L glycine, 0.1 mg/L indoleacetic acid (IAA), 6.5 g/L of agar, adjusted pH to 5.7. The experiment was evaluated after 90 days. The variables measured were: number of nodes, number of leaves, plant height (cm), number of roots and length (cm) of the root system and internodes. The period of 60 days of in vitro culture of ovules resulted in the highest values of embryos (about 50%), as well as better characterized developmental stages with higher germination (47.3%). The three types of hybrid grapes evaluated in micropropagation showed very similar values of the measured parameters, even having originated from embryos of different developmental stages. |
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Immature embryo rescue and in vitro development evaluation of intraspecific hybrids from Brazilian seedless grapevine Superior × Thompson clones.Melhoramento genéticoUva sem sementeCultivo in vitroUvaGenótipoVitis ViniferaGrapesThe fruit production for export is an economically significant activity in the Valley of São Francisco River, especially in the irrigated lands of Petrolina-PE/Juazeiro-BA, Brazil. The development of new genetic material most suitable to the tropical climate and the demands of the consumer market have led to the selection of new seedless grapes cultivars. In this case, the use of the embryo rescue technique has produced satisfactory results for obtaining such materials, especially in the semiarid region. This study aimed to evaluate the in vitro development of intraspecific hybrids of grapevine (Vitis vinifera L.), derived from the rescue of immature embryos resultant from the crossing of Superior Seedless and Thompson Seedless Brazilian clones. To establish and develop the cultivation, the culture media was supplemented with 30 g/L sucrose, 0.1 g/L myo-inositol, 0.002 g/L glycine, 0.1 mg/L indoleacetic acid (IAA), 6.5 g/L of agar, adjusted pH to 5.7. The experiment was evaluated after 90 days. The variables measured were: number of nodes, number of leaves, plant height (cm), number of roots and length (cm) of the root system and internodes. The period of 60 days of in vitro culture of ovules resulted in the highest values of embryos (about 50%), as well as better characterized developmental stages with higher germination (47.3%). The three types of hybrid grapes evaluated in micropropagation showed very similar values of the measured parameters, even having originated from embryos of different developmental stages.EIRYANNE FONSECA DE MENEZES; ELIENE MATOS E SILVA; ADRIANA MAYUMI YANO-MELO, UNIVASF; PATRICIA COELHO DE SOUZA LEAO, CPATSA; NATONIEL FRANKLIN DE MELO, CPATSA.MENEZES, E. F. deSILVA, E. M. eMELO, A. M. Y.LEAO, P. C. de S.MELO, N. F. de2014-06-20T11:11:11Z2014-06-20T11:11:11Z2014-06-2020142014-06-20T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAmerican Journal of Plant Sciences, Irvine, v. 5, p. 1956-1960, 2014.http://www.alice.cnptia.embrapa.br/alice/handle/doc/988687enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T00:41:06Zoai:www.alice.cnptia.embrapa.br:doc/988687Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T00:41:06falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T00:41:06Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Immature embryo rescue and in vitro development evaluation of intraspecific hybrids from Brazilian seedless grapevine Superior × Thompson clones. |
title |
Immature embryo rescue and in vitro development evaluation of intraspecific hybrids from Brazilian seedless grapevine Superior × Thompson clones. |
spellingShingle |
Immature embryo rescue and in vitro development evaluation of intraspecific hybrids from Brazilian seedless grapevine Superior × Thompson clones. MENEZES, E. F. de Melhoramento genético Uva sem semente Cultivo in vitro Uva Genótipo Vitis Vinifera Grapes |
title_short |
Immature embryo rescue and in vitro development evaluation of intraspecific hybrids from Brazilian seedless grapevine Superior × Thompson clones. |
title_full |
Immature embryo rescue and in vitro development evaluation of intraspecific hybrids from Brazilian seedless grapevine Superior × Thompson clones. |
title_fullStr |
Immature embryo rescue and in vitro development evaluation of intraspecific hybrids from Brazilian seedless grapevine Superior × Thompson clones. |
title_full_unstemmed |
Immature embryo rescue and in vitro development evaluation of intraspecific hybrids from Brazilian seedless grapevine Superior × Thompson clones. |
title_sort |
Immature embryo rescue and in vitro development evaluation of intraspecific hybrids from Brazilian seedless grapevine Superior × Thompson clones. |
author |
MENEZES, E. F. de |
author_facet |
MENEZES, E. F. de SILVA, E. M. e MELO, A. M. Y. LEAO, P. C. de S. MELO, N. F. de |
author_role |
author |
author2 |
SILVA, E. M. e MELO, A. M. Y. LEAO, P. C. de S. MELO, N. F. de |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
EIRYANNE FONSECA DE MENEZES; ELIENE MATOS E SILVA; ADRIANA MAYUMI YANO-MELO, UNIVASF; PATRICIA COELHO DE SOUZA LEAO, CPATSA; NATONIEL FRANKLIN DE MELO, CPATSA. |
dc.contributor.author.fl_str_mv |
MENEZES, E. F. de SILVA, E. M. e MELO, A. M. Y. LEAO, P. C. de S. MELO, N. F. de |
dc.subject.por.fl_str_mv |
Melhoramento genético Uva sem semente Cultivo in vitro Uva Genótipo Vitis Vinifera Grapes |
topic |
Melhoramento genético Uva sem semente Cultivo in vitro Uva Genótipo Vitis Vinifera Grapes |
description |
The fruit production for export is an economically significant activity in the Valley of São Francisco River, especially in the irrigated lands of Petrolina-PE/Juazeiro-BA, Brazil. The development of new genetic material most suitable to the tropical climate and the demands of the consumer market have led to the selection of new seedless grapes cultivars. In this case, the use of the embryo rescue technique has produced satisfactory results for obtaining such materials, especially in the semiarid region. This study aimed to evaluate the in vitro development of intraspecific hybrids of grapevine (Vitis vinifera L.), derived from the rescue of immature embryos resultant from the crossing of Superior Seedless and Thompson Seedless Brazilian clones. To establish and develop the cultivation, the culture media was supplemented with 30 g/L sucrose, 0.1 g/L myo-inositol, 0.002 g/L glycine, 0.1 mg/L indoleacetic acid (IAA), 6.5 g/L of agar, adjusted pH to 5.7. The experiment was evaluated after 90 days. The variables measured were: number of nodes, number of leaves, plant height (cm), number of roots and length (cm) of the root system and internodes. The period of 60 days of in vitro culture of ovules resulted in the highest values of embryos (about 50%), as well as better characterized developmental stages with higher germination (47.3%). The three types of hybrid grapes evaluated in micropropagation showed very similar values of the measured parameters, even having originated from embryos of different developmental stages. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-20T11:11:11Z 2014-06-20T11:11:11Z 2014-06-20 2014 2014-06-20T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
American Journal of Plant Sciences, Irvine, v. 5, p. 1956-1960, 2014. http://www.alice.cnptia.embrapa.br/alice/handle/doc/988687 |
identifier_str_mv |
American Journal of Plant Sciences, Irvine, v. 5, p. 1956-1960, 2014. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/988687 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503391540412416 |