Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality.
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148771 https://doi.org/10.4025/actasciagron.v45i1.56908 |
Resumo: | The reduced longevity of coffee seeds has been attributed to their sensitivity to desiccation. Studies related to gene expression and enzyme activity in coffee seeds under drying are important for understanding the effects of drying on their physiological quality. The aim of this study was to investigate the molecular aspects of seeds under different drying methods and associate them with physiological quality. Coffee seeds with different water contents were dried both slowly and rapidly. Enzymatic activity was analysed, as well as the expression of genes that encode the enzymes superoxide dismutase, catalase, peroxiredoxins, isocitrate lyase, and endo-ß-mannanase. There was a significant effect of drying speed and final water content on enzyme activity and on the expression of the different genes analysed. In seeds under rapid drying, there was greater expression of the genes that encode the enzymes catalase and endo-ß-mannanase. Greater expression of the 1 CYS PRX and SOD genes and greater activity of the ICL isoenzymes were found in seeds with superior physiological quality, but greater activity of the endo-β-mannanase and CAT enzymes occurred in seeds with lower physiological quality. |
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Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality.DryingCoffea arabica var. arabicaEnzyme activityOxidative stressThe reduced longevity of coffee seeds has been attributed to their sensitivity to desiccation. Studies related to gene expression and enzyme activity in coffee seeds under drying are important for understanding the effects of drying on their physiological quality. The aim of this study was to investigate the molecular aspects of seeds under different drying methods and associate them with physiological quality. Coffee seeds with different water contents were dried both slowly and rapidly. Enzymatic activity was analysed, as well as the expression of genes that encode the enzymes superoxide dismutase, catalase, peroxiredoxins, isocitrate lyase, and endo-ß-mannanase. There was a significant effect of drying speed and final water content on enzyme activity and on the expression of the different genes analysed. In seeds under rapid drying, there was greater expression of the genes that encode the enzymes catalase and endo-ß-mannanase. Greater expression of the 1 CYS PRX and SOD genes and greater activity of the ICL isoenzymes were found in seeds with superior physiological quality, but greater activity of the endo-β-mannanase and CAT enzymes occurred in seeds with lower physiological quality.MAYARA HOLANDA DE CARVALHO; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; STEFÂNIA VIIAS BOAS COELHO, UNIVERSIDADE FEDERAL DE LAVRAS; CRISTIANE CARVALHO GUIMARÃES, UNIVERSIDADE ESTADUAL PAULISTA; RAYANA DE SÁ MARTINS, INSTITUTO DE BOTÂNICA; ALINE DA CONSOLAÇÃO SAMPAIO CLEMENTE, ONSEEDS TREINAMENTOS; LUCIANO VILELA PAIVA, UNIVERSIDADE FEDERAL DE LAVRAS.CARVALHO, M. H. deROSA, S. D. V. F. daCOELHO, S. V. B.GUIMARÃES, C. C.MARTINS, R. de S.CLEMENTE, A. da C. S.PAIVA, L. V.2022-11-25T15:01:45Z2022-11-25T15:01:45Z2022-11-252023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleActa Scientiarum: Agronomy, v. 45, e56908, 2023.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148771https://doi.org/10.4025/actasciagron.v45i1.56908enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2022-11-25T15:01:45Zoai:www.alice.cnptia.embrapa.br:doc/1148771Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542022-11-25T15:01:45falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542022-11-25T15:01:45Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality. |
title |
Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality. |
spellingShingle |
Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality. CARVALHO, M. H. de Drying Coffea arabica var. arabica Enzyme activity Oxidative stress |
title_short |
Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality. |
title_full |
Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality. |
title_fullStr |
Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality. |
title_full_unstemmed |
Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality. |
title_sort |
Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality. |
author |
CARVALHO, M. H. de |
author_facet |
CARVALHO, M. H. de ROSA, S. D. V. F. da COELHO, S. V. B. GUIMARÃES, C. C. MARTINS, R. de S. CLEMENTE, A. da C. S. PAIVA, L. V. |
author_role |
author |
author2 |
ROSA, S. D. V. F. da COELHO, S. V. B. GUIMARÃES, C. C. MARTINS, R. de S. CLEMENTE, A. da C. S. PAIVA, L. V. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
MAYARA HOLANDA DE CARVALHO; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; STEFÂNIA VIIAS BOAS COELHO, UNIVERSIDADE FEDERAL DE LAVRAS; CRISTIANE CARVALHO GUIMARÃES, UNIVERSIDADE ESTADUAL PAULISTA; RAYANA DE SÁ MARTINS, INSTITUTO DE BOTÂNICA; ALINE DA CONSOLAÇÃO SAMPAIO CLEMENTE, ONSEEDS TREINAMENTOS; LUCIANO VILELA PAIVA, UNIVERSIDADE FEDERAL DE LAVRAS. |
dc.contributor.author.fl_str_mv |
CARVALHO, M. H. de ROSA, S. D. V. F. da COELHO, S. V. B. GUIMARÃES, C. C. MARTINS, R. de S. CLEMENTE, A. da C. S. PAIVA, L. V. |
dc.subject.por.fl_str_mv |
Drying Coffea arabica var. arabica Enzyme activity Oxidative stress |
topic |
Drying Coffea arabica var. arabica Enzyme activity Oxidative stress |
description |
The reduced longevity of coffee seeds has been attributed to their sensitivity to desiccation. Studies related to gene expression and enzyme activity in coffee seeds under drying are important for understanding the effects of drying on their physiological quality. The aim of this study was to investigate the molecular aspects of seeds under different drying methods and associate them with physiological quality. Coffee seeds with different water contents were dried both slowly and rapidly. Enzymatic activity was analysed, as well as the expression of genes that encode the enzymes superoxide dismutase, catalase, peroxiredoxins, isocitrate lyase, and endo-ß-mannanase. There was a significant effect of drying speed and final water content on enzyme activity and on the expression of the different genes analysed. In seeds under rapid drying, there was greater expression of the genes that encode the enzymes catalase and endo-ß-mannanase. Greater expression of the 1 CYS PRX and SOD genes and greater activity of the ICL isoenzymes were found in seeds with superior physiological quality, but greater activity of the endo-β-mannanase and CAT enzymes occurred in seeds with lower physiological quality. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-25T15:01:45Z 2022-11-25T15:01:45Z 2022-11-25 2023 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Acta Scientiarum: Agronomy, v. 45, e56908, 2023. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148771 https://doi.org/10.4025/actasciagron.v45i1.56908 |
identifier_str_mv |
Acta Scientiarum: Agronomy, v. 45, e56908, 2023. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148771 https://doi.org/10.4025/actasciagron.v45i1.56908 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503534939471872 |