Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality.

Detalhes bibliográficos
Autor(a) principal: CARVALHO, M. H. de
Data de Publicação: 2022
Outros Autores: ROSA, S. D. V. F. da, COELHO, S. V. B., GUIMARÃES, C. C., MARTINS, R. de S., CLEMENTE, A. da C. S., PAIVA, L. V.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148771
https://doi.org/10.4025/actasciagron.v45i1.56908
Resumo: The reduced longevity of coffee seeds has been attributed to their sensitivity to desiccation. Studies related to gene expression and enzyme activity in coffee seeds under drying are important for understanding the effects of drying on their physiological quality. The aim of this study was to investigate the molecular aspects of seeds under different drying methods and associate them with physiological quality. Coffee seeds with different water contents were dried both slowly and rapidly. Enzymatic activity was analysed, as well as the expression of genes that encode the enzymes superoxide dismutase, catalase, peroxiredoxins, isocitrate lyase, and endo-ß-mannanase. There was a significant effect of drying speed and final water content on enzyme activity and on the expression of the different genes analysed. In seeds under rapid drying, there was greater expression of the genes that encode the enzymes catalase and endo-ß-mannanase. Greater expression of the 1 CYS PRX and SOD genes and greater activity of the ICL isoenzymes were found in seeds with superior physiological quality, but greater activity of the endo-β-mannanase and CAT enzymes occurred in seeds with lower physiological quality.
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spelling Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality.DryingCoffea arabica var. arabicaEnzyme activityOxidative stressThe reduced longevity of coffee seeds has been attributed to their sensitivity to desiccation. Studies related to gene expression and enzyme activity in coffee seeds under drying are important for understanding the effects of drying on their physiological quality. The aim of this study was to investigate the molecular aspects of seeds under different drying methods and associate them with physiological quality. Coffee seeds with different water contents were dried both slowly and rapidly. Enzymatic activity was analysed, as well as the expression of genes that encode the enzymes superoxide dismutase, catalase, peroxiredoxins, isocitrate lyase, and endo-ß-mannanase. There was a significant effect of drying speed and final water content on enzyme activity and on the expression of the different genes analysed. In seeds under rapid drying, there was greater expression of the genes that encode the enzymes catalase and endo-ß-mannanase. Greater expression of the 1 CYS PRX and SOD genes and greater activity of the ICL isoenzymes were found in seeds with superior physiological quality, but greater activity of the endo-β-mannanase and CAT enzymes occurred in seeds with lower physiological quality.MAYARA HOLANDA DE CARVALHO; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; STEFÂNIA VIIAS BOAS COELHO, UNIVERSIDADE FEDERAL DE LAVRAS; CRISTIANE CARVALHO GUIMARÃES, UNIVERSIDADE ESTADUAL PAULISTA; RAYANA DE SÁ MARTINS, INSTITUTO DE BOTÂNICA; ALINE DA CONSOLAÇÃO SAMPAIO CLEMENTE, ONSEEDS TREINAMENTOS; LUCIANO VILELA PAIVA, UNIVERSIDADE FEDERAL DE LAVRAS.CARVALHO, M. H. deROSA, S. D. V. F. daCOELHO, S. V. B.GUIMARÃES, C. C.MARTINS, R. de S.CLEMENTE, A. da C. S.PAIVA, L. V.2022-11-25T15:01:45Z2022-11-25T15:01:45Z2022-11-252023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleActa Scientiarum: Agronomy, v. 45, e56908, 2023.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148771https://doi.org/10.4025/actasciagron.v45i1.56908enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2022-11-25T15:01:45Zoai:www.alice.cnptia.embrapa.br:doc/1148771Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542022-11-25T15:01:45falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542022-11-25T15:01:45Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality.
title Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality.
spellingShingle Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality.
CARVALHO, M. H. de
Drying
Coffea arabica var. arabica
Enzyme activity
Oxidative stress
title_short Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality.
title_full Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality.
title_fullStr Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality.
title_full_unstemmed Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality.
title_sort Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality.
author CARVALHO, M. H. de
author_facet CARVALHO, M. H. de
ROSA, S. D. V. F. da
COELHO, S. V. B.
GUIMARÃES, C. C.
MARTINS, R. de S.
CLEMENTE, A. da C. S.
PAIVA, L. V.
author_role author
author2 ROSA, S. D. V. F. da
COELHO, S. V. B.
GUIMARÃES, C. C.
MARTINS, R. de S.
CLEMENTE, A. da C. S.
PAIVA, L. V.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv MAYARA HOLANDA DE CARVALHO; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; STEFÂNIA VIIAS BOAS COELHO, UNIVERSIDADE FEDERAL DE LAVRAS; CRISTIANE CARVALHO GUIMARÃES, UNIVERSIDADE ESTADUAL PAULISTA; RAYANA DE SÁ MARTINS, INSTITUTO DE BOTÂNICA; ALINE DA CONSOLAÇÃO SAMPAIO CLEMENTE, ONSEEDS TREINAMENTOS; LUCIANO VILELA PAIVA, UNIVERSIDADE FEDERAL DE LAVRAS.
dc.contributor.author.fl_str_mv CARVALHO, M. H. de
ROSA, S. D. V. F. da
COELHO, S. V. B.
GUIMARÃES, C. C.
MARTINS, R. de S.
CLEMENTE, A. da C. S.
PAIVA, L. V.
dc.subject.por.fl_str_mv Drying
Coffea arabica var. arabica
Enzyme activity
Oxidative stress
topic Drying
Coffea arabica var. arabica
Enzyme activity
Oxidative stress
description The reduced longevity of coffee seeds has been attributed to their sensitivity to desiccation. Studies related to gene expression and enzyme activity in coffee seeds under drying are important for understanding the effects of drying on their physiological quality. The aim of this study was to investigate the molecular aspects of seeds under different drying methods and associate them with physiological quality. Coffee seeds with different water contents were dried both slowly and rapidly. Enzymatic activity was analysed, as well as the expression of genes that encode the enzymes superoxide dismutase, catalase, peroxiredoxins, isocitrate lyase, and endo-ß-mannanase. There was a significant effect of drying speed and final water content on enzyme activity and on the expression of the different genes analysed. In seeds under rapid drying, there was greater expression of the genes that encode the enzymes catalase and endo-ß-mannanase. Greater expression of the 1 CYS PRX and SOD genes and greater activity of the ICL isoenzymes were found in seeds with superior physiological quality, but greater activity of the endo-β-mannanase and CAT enzymes occurred in seeds with lower physiological quality.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-25T15:01:45Z
2022-11-25T15:01:45Z
2022-11-25
2023
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Acta Scientiarum: Agronomy, v. 45, e56908, 2023.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148771
https://doi.org/10.4025/actasciagron.v45i1.56908
identifier_str_mv Acta Scientiarum: Agronomy, v. 45, e56908, 2023.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148771
https://doi.org/10.4025/actasciagron.v45i1.56908
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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