The acute effect of intravenous lipopolysaccharide injection on serumand intrafollicular HDL components and gene expression in granulosacells of the bovine dominant follicle.

Detalhes bibliográficos
Autor(a) principal: CAMPOS, F. T. de
Data de Publicação: 2017
Outros Autores: RINCON, J. A. A., ACOSTA, D. A. V., SILVEIRA, P. A. S., PRADIEÉ, J., CORRÊA, M. N., GASPERIN, B. G., PFEIFER, L. F. M., BARROS, C. C., PEGORARO, L. M. C., SCHNEIDER, A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1063230
Resumo: The aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) ? estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 &#956;g/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal. Blood, granulosa cells and FF samples were collected six hours after LPS injection. Five hours after LPS injection rectal temperature was increased in LPS (P < 0.0001, 40.4 ± 0.1 °C) compared to the CTL cows (38.8 ± 0.1 °C). Serum PON1 activity was reduced by LPS injection (130.2 ± 5.1 vs. 99.6 ± 3.3 U/mL; P < 0.001), as well as HDL-cholesterol concentrations (70.3 ± 5.3 vs. 50.1 ± 6.2 mg/dL; P < 0.05). The FF E2 and P4 concentrations were not different between groups (P > 0.05). The PON1 activity in the FF was also decreased by LPS injection (P = 0.01). In comparison to CTL group, cows injected with LPS had a ten fold reduction in STAR, TLR4 and TNF mRNA expression (P < 0.05). In conclusion, an intravenous LPS challenge in cows induced an acute systemic inflammatory response reducing HDL and its components in serum but not in the FF. Only PON1 activity serum reduction was reflected in the FF in the short term. Additionally, steroidogenic and inflammatory genes had reduced expression in the granulosa cells.
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spelling The acute effect of intravenous lipopolysaccharide injection on serumand intrafollicular HDL components and gene expression in granulosacells of the bovine dominant follicle.PON1EsteroidogêneseColesterolInflamaçãocholesterolinflammationsteroidogenesisThe aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) ? estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 &#956;g/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal. Blood, granulosa cells and FF samples were collected six hours after LPS injection. Five hours after LPS injection rectal temperature was increased in LPS (P < 0.0001, 40.4 ± 0.1 °C) compared to the CTL cows (38.8 ± 0.1 °C). Serum PON1 activity was reduced by LPS injection (130.2 ± 5.1 vs. 99.6 ± 3.3 U/mL; P < 0.001), as well as HDL-cholesterol concentrations (70.3 ± 5.3 vs. 50.1 ± 6.2 mg/dL; P < 0.05). The FF E2 and P4 concentrations were not different between groups (P > 0.05). The PON1 activity in the FF was also decreased by LPS injection (P = 0.01). In comparison to CTL group, cows injected with LPS had a ten fold reduction in STAR, TLR4 and TNF mRNA expression (P < 0.05). In conclusion, an intravenous LPS challenge in cows induced an acute systemic inflammatory response reducing HDL and its components in serum but not in the FF. Only PON1 activity serum reduction was reflected in the FF in the short term. Additionally, steroidogenic and inflammatory genes had reduced expression in the granulosa cells.Felipe Terres de Campos, UFPel; Joao Aveiro Alvarado Rincon, UFPel; Diego Andres Velasco Acosta, CORPOICA; Pedro Augusto Silva Silveira, UFPel; Jorgea Pradieé, Embrapa Clima Temperado; Marcio Nunes Corrêa, UFPel; Bernardo Garziera Gasperin, UFPel; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-Rondonia; Carlos Castilho Barros, UFPel; LIGIA MARGARETH CANTARELLI PEGORARO, CPACT; Augusto Schneider, UFPel.CAMPOS, F. T. deRINCON, J. A. A.ACOSTA, D. A. V.SILVEIRA, P. A. S.PRADIEÉ, J.CORRÊA, M. N.GASPERIN, B. G.PFEIFER, L. F. M.BARROS, C. C.PEGORARO, L. M. C.SCHNEIDER, A.2021-11-10T15:00:26Z2021-11-10T15:00:26Z2017-02-082017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleTheriogenology, v. 89, p. 244 - 249, 2017.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1063230enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2021-11-10T15:00:36Zoai:www.alice.cnptia.embrapa.br:doc/1063230Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542021-11-10T15:00:36falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542021-11-10T15:00:36Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv The acute effect of intravenous lipopolysaccharide injection on serumand intrafollicular HDL components and gene expression in granulosacells of the bovine dominant follicle.
title The acute effect of intravenous lipopolysaccharide injection on serumand intrafollicular HDL components and gene expression in granulosacells of the bovine dominant follicle.
spellingShingle The acute effect of intravenous lipopolysaccharide injection on serumand intrafollicular HDL components and gene expression in granulosacells of the bovine dominant follicle.
CAMPOS, F. T. de
PON1
Esteroidogênese
Colesterol
Inflamação
cholesterol
inflammation
steroidogenesis
title_short The acute effect of intravenous lipopolysaccharide injection on serumand intrafollicular HDL components and gene expression in granulosacells of the bovine dominant follicle.
title_full The acute effect of intravenous lipopolysaccharide injection on serumand intrafollicular HDL components and gene expression in granulosacells of the bovine dominant follicle.
title_fullStr The acute effect of intravenous lipopolysaccharide injection on serumand intrafollicular HDL components and gene expression in granulosacells of the bovine dominant follicle.
title_full_unstemmed The acute effect of intravenous lipopolysaccharide injection on serumand intrafollicular HDL components and gene expression in granulosacells of the bovine dominant follicle.
title_sort The acute effect of intravenous lipopolysaccharide injection on serumand intrafollicular HDL components and gene expression in granulosacells of the bovine dominant follicle.
author CAMPOS, F. T. de
author_facet CAMPOS, F. T. de
RINCON, J. A. A.
ACOSTA, D. A. V.
SILVEIRA, P. A. S.
PRADIEÉ, J.
CORRÊA, M. N.
GASPERIN, B. G.
PFEIFER, L. F. M.
BARROS, C. C.
PEGORARO, L. M. C.
SCHNEIDER, A.
author_role author
author2 RINCON, J. A. A.
ACOSTA, D. A. V.
SILVEIRA, P. A. S.
PRADIEÉ, J.
CORRÊA, M. N.
GASPERIN, B. G.
PFEIFER, L. F. M.
BARROS, C. C.
PEGORARO, L. M. C.
SCHNEIDER, A.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Felipe Terres de Campos, UFPel; Joao Aveiro Alvarado Rincon, UFPel; Diego Andres Velasco Acosta, CORPOICA; Pedro Augusto Silva Silveira, UFPel; Jorgea Pradieé, Embrapa Clima Temperado; Marcio Nunes Corrêa, UFPel; Bernardo Garziera Gasperin, UFPel; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-Rondonia; Carlos Castilho Barros, UFPel; LIGIA MARGARETH CANTARELLI PEGORARO, CPACT; Augusto Schneider, UFPel.
dc.contributor.author.fl_str_mv CAMPOS, F. T. de
RINCON, J. A. A.
ACOSTA, D. A. V.
SILVEIRA, P. A. S.
PRADIEÉ, J.
CORRÊA, M. N.
GASPERIN, B. G.
PFEIFER, L. F. M.
BARROS, C. C.
PEGORARO, L. M. C.
SCHNEIDER, A.
dc.subject.por.fl_str_mv PON1
Esteroidogênese
Colesterol
Inflamação
cholesterol
inflammation
steroidogenesis
topic PON1
Esteroidogênese
Colesterol
Inflamação
cholesterol
inflammation
steroidogenesis
description The aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) ? estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 &#956;g/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal. Blood, granulosa cells and FF samples were collected six hours after LPS injection. Five hours after LPS injection rectal temperature was increased in LPS (P < 0.0001, 40.4 ± 0.1 °C) compared to the CTL cows (38.8 ± 0.1 °C). Serum PON1 activity was reduced by LPS injection (130.2 ± 5.1 vs. 99.6 ± 3.3 U/mL; P < 0.001), as well as HDL-cholesterol concentrations (70.3 ± 5.3 vs. 50.1 ± 6.2 mg/dL; P < 0.05). The FF E2 and P4 concentrations were not different between groups (P > 0.05). The PON1 activity in the FF was also decreased by LPS injection (P = 0.01). In comparison to CTL group, cows injected with LPS had a ten fold reduction in STAR, TLR4 and TNF mRNA expression (P < 0.05). In conclusion, an intravenous LPS challenge in cows induced an acute systemic inflammatory response reducing HDL and its components in serum but not in the FF. Only PON1 activity serum reduction was reflected in the FF in the short term. Additionally, steroidogenic and inflammatory genes had reduced expression in the granulosa cells.
publishDate 2017
dc.date.none.fl_str_mv 2017-02-08
2017
2021-11-10T15:00:26Z
2021-11-10T15:00:26Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Theriogenology, v. 89, p. 244 - 249, 2017.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1063230
identifier_str_mv Theriogenology, v. 89, p. 244 - 249, 2017.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1063230
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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