Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe.

Detalhes bibliográficos
Autor(a) principal: FAJARDO, T. V. M.
Data de Publicação: 2014
Outros Autores: NICKEL, O.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/999019
Resumo: The viruses Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV) and Apple mosaic virus (ApMV) are common in apples and pears and main targets of detection in propagation materials. This study aimed at demonstrating the usefulness of the hybridization method with a non-radioactive probe for simultaneous detection of these four viruses. The sensitivity of this method was suffi ciently high enabling the detection of ASGV, ACLSV, ASPV and ApMV in total RNA extracted from infected samples. The probe specifi city was confi rmed by reaction with homologous viral cDNA, individually cloned for each virus.
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spelling Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe.ASGVACLSVASPVApMVMacieiraPolissondaMaçãDoença de plantaHibridaçãoApple chlorotic leaf spot virusApple mosaic virusApple stem grooving virusApple stem pitting virusThe viruses Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV) and Apple mosaic virus (ApMV) are common in apples and pears and main targets of detection in propagation materials. This study aimed at demonstrating the usefulness of the hybridization method with a non-radioactive probe for simultaneous detection of these four viruses. The sensitivity of this method was suffi ciently high enabling the detection of ASGV, ACLSV, ASPV and ApMV in total RNA extracted from infected samples. The probe specifi city was confi rmed by reaction with homologous viral cDNA, individually cloned for each virus.THOR VINICIUS MARTINS FAJARDO, CNPUV; OSMAR NICKEL, CNPUV.FAJARDO, T. V. M.NICKEL, O.2014-11-03T11:11:11Z2014-11-03T11:11:11Z2014-11-0320142014-11-03T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCiência Rural, Santa Maria, v. 44, n. 10, p. 1711-1714, out. 2014.http://www.alice.cnptia.embrapa.br/alice/handle/doc/999019enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T00:20:13Zoai:www.alice.cnptia.embrapa.br:doc/999019Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T00:20:13falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T00:20:13Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe.
title Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe.
spellingShingle Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe.
FAJARDO, T. V. M.
ASGV
ACLSV
ASPV
ApMV
Macieira
Polissonda
Maçã
Doença de planta
Hibridação
Apple chlorotic leaf spot virus
Apple mosaic virus
Apple stem grooving virus
Apple stem pitting virus
title_short Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe.
title_full Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe.
title_fullStr Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe.
title_full_unstemmed Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe.
title_sort Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe.
author FAJARDO, T. V. M.
author_facet FAJARDO, T. V. M.
NICKEL, O.
author_role author
author2 NICKEL, O.
author2_role author
dc.contributor.none.fl_str_mv THOR VINICIUS MARTINS FAJARDO, CNPUV; OSMAR NICKEL, CNPUV.
dc.contributor.author.fl_str_mv FAJARDO, T. V. M.
NICKEL, O.
dc.subject.por.fl_str_mv ASGV
ACLSV
ASPV
ApMV
Macieira
Polissonda
Maçã
Doença de planta
Hibridação
Apple chlorotic leaf spot virus
Apple mosaic virus
Apple stem grooving virus
Apple stem pitting virus
topic ASGV
ACLSV
ASPV
ApMV
Macieira
Polissonda
Maçã
Doença de planta
Hibridação
Apple chlorotic leaf spot virus
Apple mosaic virus
Apple stem grooving virus
Apple stem pitting virus
description The viruses Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV) and Apple mosaic virus (ApMV) are common in apples and pears and main targets of detection in propagation materials. This study aimed at demonstrating the usefulness of the hybridization method with a non-radioactive probe for simultaneous detection of these four viruses. The sensitivity of this method was suffi ciently high enabling the detection of ASGV, ACLSV, ASPV and ApMV in total RNA extracted from infected samples. The probe specifi city was confi rmed by reaction with homologous viral cDNA, individually cloned for each virus.
publishDate 2014
dc.date.none.fl_str_mv 2014-11-03T11:11:11Z
2014-11-03T11:11:11Z
2014-11-03
2014
2014-11-03T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Ciência Rural, Santa Maria, v. 44, n. 10, p. 1711-1714, out. 2014.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/999019
identifier_str_mv Ciência Rural, Santa Maria, v. 44, n. 10, p. 1711-1714, out. 2014.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/999019
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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